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Citations for R&D Systems :
1901 - 1950 of 1951 citations for Probable protein BRICK1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen, Germany; R&D Systems, Minneapolis, USA) and a horseradish peroxidase (HRP)-coupled secondary antibody and CheLuminate-HRP ECL solution (Applichem ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... P0 were fixed and histologically sectioned and were stained with antibodies to detect Wnt4 (anti-Wnt4, R&D systems, cat# AF475, dilution 1:100), Wnt9b (anti-Wnt9b ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4℃ with the following primary antibodies: anti-Nestin (rabbit, 1:500, Millpore, PN-MAB5326) and anti-ki67 (mouse, 1:500, R&D system, PN-AF7649); anti-CHI3L1 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2020Quote: The primary antibodies used were Collagen IV (1:400 dilution; Cosmo Bio, Catalog Number LSL-LB1403) and CD13 (1:100 dilution; R&D Systems, Catalog Number AF2335). Mice were sacrificed by decapitation ...
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bioRxiv - Genomics 2020Quote: ... LDLR staining was performed for 30 min on ice with a 1:50 dilution of AlexaFluor488-conjugated LDLR antibody (R&D Systems, Minneapolois MN, FAB2148G) into PBS supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957; iNOS, Abcam, Cat# ab15323; CD206, R&D Systems, Cat# AF2535) in TBST containing 2% BSA for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using the following primary and secondary antibodies: anti-IL-1β (R&D systems, AF-201-NA), anti-GFP (Clontech ...
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bioRxiv - Developmental Biology 2022Quote: ... In-house purified rhFGF10 was confirmed by western blot analysis using anti-FGF10 antibody and compared to commercially purchased rhFGF10 (R&D Systems, Cat#345-FG) to test/validate activity based on the efficiency to phosphorylate ERK1/2 in an A549 alveolar epithelial cell line (ATCC ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
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bioRxiv - Developmental Biology 2019Quote: ... blocked (5 % BSA and 0.1 % Tween 20 in 1X TBS) for at least 1 hour at room temperature and stained with primary antibodies (Phospho-STAT3 Y705 and STAT3, R&D Systems 9131S and 9132S) diluted in blocking solution overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Developmental Biology 2019Quote: Western blotting was performed as previously described (Chang et al., 2016, Mariani et al., 2016, Bay et al., 2018) with the following antibodies: Gli2 (R&D Systems, AF3635, 1:500), Gli3 (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Molecular Biology 2022Quote: ... transferred to a fresh reaction tube and subsequently washed three times with 500 μl PBS-T before addition of primary antibody (R&D systems, Minneapolis, MN, USA) 1:5000 in 200 μl sample buffer ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
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bioRxiv - Pathology 2023Quote: ... CXCL2, CXCL1, TNF, MMP8, S100A8, IFN-γ, IL12p40, IL-12p70, IL-10, and VEGF using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transferred to wet ice and the remaining surface receptor was labeled with 1:100 anti-rabbit antibody conjugated to PE (F0110, R&D Systems, Minneapolis, MN, USA) in FACS buffer for 30 min on ice and protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981; IFNλ2/3: R&D systems, MAB1587), or mock ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Following primary antibodies (αTFAP2A, Abcam ab108311; αTFAP2B, Abcam ab221094; αLEF1, Cell Signaling, 2230; αSOX2, R&D Systems, AF2018; αPITX1, Abcam ab70273; and αPITX2, R&D Systems, AF7388) were diluted in TBS (0.5% Tween in phosphate-buffered saline ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were isolated and stained with anti- human CD3 and CD8a antibodies (surface staining) followed by intracellular staining with anti- human IFNγ (R&D Systems, Cat. No. IC285F-100) antibody ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... RBCs were washed twice with ice cold RBC staining buffer (PBS supplemented with 0.2% BSA) before staining on ice with anti-CD59AF405 monoclonal antibody (FAB1987V, R&D Systems, 5 ng/µL final concentration) and anti-C3c-FITC polyclonal antibody (ab4212 ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...