Labshake search
Citations for R&D Systems :
1851 - 1900 of 1929 citations for TPBG Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
-
bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... proteins were extracted via lysis using a lysis buffer from the Proteome Profiler™ Human Apoptosis Array Kit (ARY009, R&D Systems, Minneapolis, MN) kit ...
-
bioRxiv - Microbiology 2023Quote: The levels of CX3CL1 were determined according to manufacturer’s instructions using the Human CX3CL1/Fractalkine DuoSet ELISA and DuoSet ELISA Ancillary Reagent Kit (R&D Systems, Minneapolis, Minnesota, USA). Supernatants were diluted 1:1 with Reagent Diluent for all measurements and performed in duplicates ...
-
bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
-
bioRxiv - Cancer Biology 2024Quote: Protein profiling of the secreted factors from each cell culture was performed using the Proteome Profiler Human XL Cytokine array kit (R&D systems, Minneapolis, MN) according to the manufacturer’s recommended protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... The iPSCs were functionally characterized by checking for their ability to differentiate into the three germ layers (Human pluripotent stem cell functional identification kit, R&D systems, Cat#SC027B) according to the manufactures protocol ...
-
bioRxiv - Cancer Biology 2024Quote: Human CCL2/MCP-1 and M-CSF/CSF1 were measured by ELISA using DuoSet kits (DY279, DY216, respectively) from R&D Systems (Minneapolis, USA) according to the manufacturer’s recommendations.
-
bioRxiv - Immunology 2024Quote: ... 100 µl of each serum diluted were added to wells of polystyrene plates coated with human IFN-α2a and IFN-β antigens (R&D Systems Inc). All serum samples were run twice in duplicate ...
-
bioRxiv - Cell Biology 2020Quote: ... dPSCs were differentiated into each of the three germ layers according to the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems®, Catalog # SC027B).
-
bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
-
bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were subsequently washed with PBS without Ca2+ and Mg2+ and then incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat ...
-
bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems ...
-
bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
-
bioRxiv - Immunology 2019Quote: ... ELISAs were performed according to the manufacturer’s instructions for IL-10 (Quantikine®HS ELISA Human IL-10 Immunoassay, R&D Systems, Inc., Cat# HS100B), sCD14 (Quantikine® ELISA Human sCD14 Immunoassay ...
-
bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
-
bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
-
bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
-
bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
-
bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
-
bioRxiv - Microbiology 2020Quote: ... cell-free supernatants were collected and used to evaluate the concentrations of TGF-β1 with human and rat TGF-β1 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
-
bioRxiv - Physiology 2020Quote: ... The measurement was performed by Enzyme-Linked Immunosorbent Assay (ELISA) using the Human PCSK9 Quantikine ELISA kit (R&D Systems, Catalog no. DPC900, Minneapolis, USA) according to kit instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
-
bioRxiv - Cell Biology 2022Quote: The relative levels of angiogenesis-related cytokines in the MSC-CM were analysed using the Proteome Profiler Human Cytokine Array Kit from R&D systems (Abingdon, UK, ARY022B) per manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
-
bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
-
bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
-
bioRxiv - Cell Biology 2024Quote: ... Mouse tibialis anterior muscles were injected with 50 uL solution of 50,000 human FAPs resuspended in 50% glycerol in PBS with either 29ng/ul concentration of recombinant DLK1 resuspended in PBS (R&D Systems, #1144-PR-025) or with control (identical volume of PBS ...
-
bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
-
bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
-
bioRxiv - Cancer Biology 2024Quote: ... lysates of specific conditions were collected and the relative phosphorylation status of 37 kinase phosphorylation sites was evaluated by using a Human Phospho-kinase Array kit (ARY003C, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
-
bioRxiv - Immunology 2023Quote: Cells were stimulated for 18-24h in complete medium at 37˚C with addition of 50- 100U/ml human IFNγ (R&D Systems #285-IF-100).
-
bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... IMR90 fibroblasts at 70-80 % confluent were serum starved overnight and treated with 50ng/ml of recombinant human PDGF-DD (R&D system, 1159-SB-025) for 24h.
-
bioRxiv - Microbiology 2023Quote: ... The concentration of IL-8 released in basolateral culture media were assayed by ELISA using the human ELISA development kits (DuoSet DY208, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
-
bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
-
bioRxiv - Biochemistry 2023Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
-
bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
-
bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
-
bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...