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Citations for R&D Systems :
1851 - 1900 of 2055 citations for GAD65 B78 Human Monoclonal Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... MSCs were characterized based on the criteria set out by the International Society for Cellular Therapy (ISCT)(ref) using the Human Mesenchymal Stem Cell Functional Identification Kit (R&D Systems) and Human Mesenchymal Stem Cell Verification kit (R&D Systems).
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bioRxiv - Bioengineering 2023Quote: ... pulled secretome for each group was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (ARY007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... We incubated 1.5ml of media on Protease Arrays (R&D Systems Proteome Profiler Human Protease Array Kit, Cat # ARY021B; R&D Systems Inc. ...
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bioRxiv - Genetics 2024Quote: ... TGFB2 dosage in each eye (13 controls + 2 cases) was conducted with the Human TGF-beta 2 Quantikine ELISA kit (R&D Systems), following the provided instructions.
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bioRxiv - Microbiology 2024Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1(R&D systems, Cat# E-522B), 2 ...
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bioRxiv - Neuroscience 2024Quote: ... SCs were then seeded on poly-L-lysine/laminin coated coverslips or petri dishes and kept in the above-mentioned medium containing recombinant human neuregulin1-ß1/heregulin1-ß1 (0.1 mg/ml, R&D systems, 396HB) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 24 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Cancer Biology 2024Quote: ... IL-6 concentrations in tissue lysates and CAF supernatants were measured using a commercially available kit (Human IL-6 DuoSet ELISA, R&D Systems). SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Duplicates of 50 μl of concentrate were subjected to IFN-β measurement by ELISA (Human IFN-beta DuoSET ELISA, R&D Systems), after 20X upconcentration (Amicon Ultracel-10 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... and acute phase proteins (total 36) present in the supernatants were detected with using Proteome Profiler Human Cytokines Array Kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... then maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems). For the pilot experiment in which CD4 cells with a defined CAR BC were spiked into CD8 cells (Figure 1D-F) ...
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bioRxiv - Immunology 2024Quote: ... All primary cells were maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems) at 37°C in 5% CO2.
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bioRxiv - Immunology 2024Quote: ... and IgG in the culture supernatants from stimulated B cells were determined by ELISA kits for human MPO (R&D Systems, #DY3174), mouse MPO (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Cell Biology 2024Quote: ... and MCP-1 were determined in efflux collected on day 8 using a custom version of the Human XL Cytokine Performance Panel (R&D systems). SHBG levels were quantified using the Human XL Cytokine Discovery Panel (R&D systems) ...
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bioRxiv - Cell Biology 2024Quote: The enzyme-linked immunosorbent assay (ELISA) for human SPARC was performed using ready-to-go sandwitch assay kit with positive controls purchased from R&D Systems and following manifacturer’s guadlines ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: L-FABP levels in human and mouse sera were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Epidemiology 2019Quote: ... HGF concentration in rinse water samples was measured by using HGF ELISA kit (Quantikine human HGF immunoassay, R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Plasma IL-6 was assessed in duplicate using high-sensitivity enzyme-linked immunosorbent assay (ELISA; Quantikine HS Human IL-6 ELISA, R&D Systems, UK) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Soluble protease expression profile of MRC-5 and hTERT-HGF 2D and 3D conditions were performed using a Human Protease Array kit according to manufacturer’s guidelines (R&D Systems, Minneapolis, MI). Images were acquired on a LAS-4000 Fujifilm imager and analyzed after subtraction of background using “Protein Array Analyzer” plugin in (Carpentier and Henault ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme-linked immunosorbent assay for human LTα was performed in triplicate using the DUO set ELISA kit (DY210, R&D Systems, Abingdon, UK). Protein was extracted from transfected meningeal cells using RIPA buffer (R0278 ...
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bioRxiv - Neuroscience 2021Quote: ... The contribution of BDNF/TrkB signaling was tested by bath application of a scavenger of endogenous BDNF (recombinant human TrkB-Fc chimera, R&D Systems, Germany). For scavenging of BDNF ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
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bioRxiv - Neuroscience 2021Quote: The relative phosphorylation levels of various kinases were measured using a Proteome Profiler Array human phospho-kinase array kit (R&D Systems #ARY003C) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1, 1:50,000, R&D Systems – AF3787). After 2x10 minutes washes with milliQ water and 2x10 minutes washes with TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of 105 proteins in supernatants was evaluated using human cytokine and chemokine XL proteome array kit from R&D Systems (Minneapolis, MN).
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bioRxiv - Cancer Biology 2020Quote: ... Then we collected the supernatants and cell lysates so that we could detect the chemokines using a Proteome Profiler Human Chemokine Array Kit (R&D Systems, ARY017).
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bioRxiv - Immunology 2022Quote: ... Detection of bound sIL7R was carried out by one-hour incubation at room temperature with biotinylated goat anti-human IL7R polyclonal antibody (R&D Systems, # BAF306), followed by 30-minute incubation at room temperature with streptavidin-horseradish peroxidase (Millipore Sigma ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted and resuspended in 100 µL FACS buffer (2% FBS in DPBS) containing 0.2 µg goat-α-human Ace-2 (R&D Systems #AF933). After 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: CCL22/Ccl22 ELISAs were performed on culture supernatants (undiluted or diluted when necessary) using Immulon 4HBX flat bottom microtiter plates with the human CCL22/MDC DuoSet ELISA (R&D Systems DY336) or the mouse Ccl22/Mdc DuoSet ELISA (R&D Systems DY439 ...
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bioRxiv - Cancer Biology 2020Quote: Angiogenesis secreted mediators were identified from fallopian tube secretory cells and ovarian carcinoma cells conditioned media employing the Human Angiogenesis Antibody Array (R&D systems: ARY007). ELISA was employed for the precise quantification of VEGF (R&D systems ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... to remove debris and processed according to the manufacturers’ instructions (Proteome Profiler™ Human XL cytokine array kit; R&D Systems, Abingdon, UK). In total ...
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bioRxiv - Cancer Biology 2022Quote: ... with good affinity and specificity were screened and selected from the homemade human single-chain antibody library through commercial VEGF and EGFR proteins (R&D Systems, Inc.). Then ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Cancer Biology 2019Quote: ... CCN4 concentration in conditioned medium was determined by ELISA using Human WISP-1/CCN4 DuoSet ELISA Development Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Fc Tag 15μg/ml (CDO-H5257, Acro Biosystem Newark, USA) or Recombinant Human IgG1 Fc 3μg/ml (110-HG, R&D Systems, Minneapolis, MN, USA) as control ...