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Citations for R&D Systems :
1801 - 1831 of 1831 citations for SCG3 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates of specific conditions were collected and the relative phosphorylation status of 37 kinase phosphorylation sites was evaluated by using a Human Phospho-kinase Array kit (ARY003C, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
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bioRxiv - Cell Biology 2024Quote: ... Mouse tibialis anterior muscles were injected with 50 uL solution of 50,000 human FAPs resuspended in 50% glycerol in PBS with either 29ng/ul concentration of recombinant DLK1 resuspended in PBS (R&D Systems, #1144-PR-025) or with control (identical volume of PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Neuroscience 2019Quote: The secretion of cytokines in AD TREM2 neuronal cultures before and after stimulation with the Aß-S8C dimer was measured employing the Proteome Profiler Human Cytokine Array kit (R&D System, United States of America). The assay was performed following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... IP-10 and TGF-β levels were measured by enzyme-linked immunosorbent assay (ELISA) sandwich using human CXCL-10/IP-10 DuoSet ELISA (R&D Systems Inc, MN, USA) and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation ...
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... IFN-γ concentrations in cell-free supernatants were determined by Human IFN-γ DuoSet enzyme-linked immunosorbent assay (ELISA; DY285; R&D systems, Minneapolis, MN, USA). EYFP−/EBFP+ dLP-CHO cells with no anti-hPD1 payload circuit were used as the control cells.
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bioRxiv - Cell Biology 2022Quote: The presence of angiogenic factors in BEC CM was evaluated trough a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D Systems Inc., Minneapolis, MN, USA). For this ...
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bioRxiv - Physiology 2020Quote: Serum samples were assayed for IL-6 concentration using commercially available human IL-6 enzyme linked immunosorbent assay kits (Quantikine®, R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Neuroscience 2024Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
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bioRxiv - Physiology 2023Quote: ... The supernatants were diluted when necessary to be in the optimal linear optical range and analyzed using commercial kits for IFNγ (Human IFN-gamma DuoSet ELISA, R&D Systems, USA and Bio legend, UK), and IL-10 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... were washed and resuspended in PBS with 0.02% Tween-20 (PBS-T) and loaded with recombinant human IL13Rα2-Fc chimera (R&D Systems Cat#614INS; 2.1 µg per 3.9×107 beads) by incubation for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
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bioRxiv - Microbiology 2023Quote: Catalytic activities of SpyCEP constructs were measured through detection of remaining intact CXCL8 or CXCL1 substrate following incubation with SpyCEP by ELISA (R&D Systems human CXCL8 and CXCL1 DuoSet ELISA) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...