Labshake search
Citations for R&D Systems :
1801 - 1850 of 2235 citations for FGF 6 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
-
bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
-
bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
-
bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
-
bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
-
bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
-
bioRxiv - Immunology 2023Quote: ... and 50 μg/ml L-Ascorbic Acid)) supplemented with Growth Factor Cocktail (5 ng/ml BMP4, 5 ng/ml bFGF, 10 ng/ml IL-6 (R&D systems, 206-IL), 10 ng/ml Flt3L (PeproTech ...
-
bioRxiv - Immunology 2023Quote: Bronchoalveolar lavage fluid or conditioned culture media were collected for measurement of IL-6 with a Duoset ELISA kit (R&D Systems, DY406-05). Capture antibody was coated overnight in a 96 well plate at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant human IL-21 was from Preprotech (200-21) and IL-21 neutralizing antibody was from R&D Systems (991-R2).
-
bioRxiv - Systems Biology 2019Quote: ... Sera were subjected to depletion of abundant serum proteins using Proteome Purify 12 Human Serum Protein Immunodepletion Resin (R&D Systems). Denaturing was followed by alkylation with N-ethylmaleimide and acetone precipitation ...
-
bioRxiv - Immunology 2020Quote: ... culture supernatants were collected and cytokine concentrations measured using CXCL10/IP10 (Human CXCL10/IP-10 Quantikine ELISA Kit, R&D Systems) and IFN-β (VeriKine-HS Human IFN Beta Serum ELISA Kit Cat No 41415 ...
-
bioRxiv - Immunology 2019Quote: ... Soluble CD14 (sCD14) concentration in plasma samples was measured using the human CD14 Quantikine ELISA kit from R&D systems (DC140) according to manufacturer’s protocol.
-
bioRxiv - Microbiology 2019Quote: ... was added to 2mL of fresh differentiation media and either 50 ng/µl of human IFNγ 285-IF (R&D systems) was added or not ...
-
bioRxiv - Bioengineering 2021Quote: ... Serum IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
-
bioRxiv - Neuroscience 2021Quote: Human soluble E-selectin levels were assayed using quantitative sandwich enzyme immunoassay technique and following the manufacturer’s protocol (Quantikine ELISA Human E-selectin/CD62E Immunoassay, DSLE00, R&D Systems). Briefly ...
-
bioRxiv - Neuroscience 2020Quote: ... or the indicated compounds were added at 12.5µM and incubated for 30 min at room temperature prior to adding biotinylated human VEGF-A165 (Cat#BT293, R&D systems) at 10 nM ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were later determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Neuroscience 2021Quote: ... Sigma-Aldrich)-coated dishes in DMEM/F12/ B27/N2-medium supplemented with 200 ng/ml human recombinant sonic hedgehog (SHH, R&D Systems) and 100 ng/ml murine recombinant fibroblast growth factor 8b (FGF-8b ...
-
bioRxiv - Bioengineering 2021Quote: ... Total TGF-β1 levels were assessed using Human TGF-beta 1 DuoSet® ELISA (DY240; R&D Systems™, Minneapolis, MN). Similarly ...
-
bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
-
bioRxiv - Microbiology 2021Quote: ... IFNβ and IFNλ protein levels from HCT-8 cultures were measured by Human IFN-beta DuoSet ELISA (DY814, R&D Systems) and Human IL29/IL28B (IFN-lambda 1/3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lambda interferon was evaluated by Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Interleukin-6 was measured by Human IL-6 DuoSet ELISA (DY206 ...
-
bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
-
bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
-
PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistancebioRxiv - Cancer Biology 2022Quote: ... epithelial to mesenchymal transition (EMT) was induced with 200 pM of Human recombinant TGF-beta 1 (R&D Systems, 240-B); verteporfin (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... Secretion of angiogenesis-related protein levels was detected using a Proteome Profiler™ Human Angiogenesis Antibody Array kit (R&D Systems) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Group 2 ligands were derived from human transcriptomic data and included: TGFβ2 (R&D Systems, Cat. 302-B2-002, 5ng/mL), NLGN1 (Sino Biological ...
-
bioRxiv - Immunology 2022Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
-
bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
-
bioRxiv - Developmental Biology 2019Quote: ... and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems) and StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR analysis of pluripotency markers was done using the Human Pluripotent Stem Cell Assessment Primer Pair Panel (R&D Systems, SC012) and amplification was done using GoTaq Green ...
-
bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
-
bioRxiv - Microbiology 2020Quote: ... PSA was measured in lateral vaginal wall swabs using Human Kallikrein 3/PSA Quantikine ELISA kits (R&D Systems, MN, USA).
-
bioRxiv - Cancer Biology 2020Quote: ... neutralizing Ab anti-human/mouse SDF-1 25μg/ml (MAB310) and mouse VEGF 150ng/ml (VEGF164) (all from R&D System).
-
bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed twice in PBS and stained with a goat anti-human ACE2 polyclonal antibody (R&D Systems, Cat # AF933) or isotype goat polyclonal isotype control (R&D Systems ...
-
bioRxiv - Microbiology 2020Quote: ... the wells were washed and then incubated with human SP-D detecting antibody (0.5 μg/ml) (R&D Systems; 1:180) for 2 h at room temperature ...
-
bioRxiv - Immunology 2020Quote: Chemokine and cytokine protein quantification were performed using Proteome Human and Mouse Cytokine Array kits (R&D Systems, San Diego, CA) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Cell culture supernatant levels of IFNg were quantified using Human IFN-gamma Quantikine ELISA Kit and manufacturer’s recommendations (DIF50, R&D Systems).
-
bioRxiv - Immunology 2020Quote: Cytokines and chemokines were measured using Human Magnetic Luminex Assay according to the manufacturer’s instructions (#LKTM014, R&D systems, MN, USA). Samples that were above the upper limit of quantification were set at the upper detection limit of the assay ...
-
bioRxiv - Cancer Biology 2022Quote: ... 300 μg cell lysates were used for phosphorylation analysis with Human Phospho-Kinase Array Kit (ARY003B, R&D Systems, Minneapolis, MN) following the manufacturer’s instruction.
-
bioRxiv - Bioengineering 2022Quote: The presence of selected cytokines was evaluated in samples of conditioned medium with Proteome profiler human angiogenesis array (R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: Supernatants from transfected HEK293T cells were analyzed for their LBP content by Human LBP DuoSet ELISA (#DY870-05, R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Purified recombinant NRX1β ectodomain fused to human immunogloblin Fc (NRX1β-Fc, R&D sytems, Cat#5268-NX-05, R&D system) was immobilized on carboxymethylated dextran CM5 sensor chips (Cytiva ...
-
bioRxiv - Physiology 2023Quote: ... Plasma and urine GDF15 were measured by ELISA test (MG150 and DGD150, for rodent and human GDF15, respectively, R&D Systems) and tissue GDF15 was measured using an ELISA test (Elabscience).
-
bioRxiv - Neuroscience 2022Quote: ... the media was incubated (72 h) with media containing different concentration of recombinant human PTN (rhPTN, R&D systems 252 - PL) or the drugs alectinib (Toronto Research Chemicals ...
-
bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
-
bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...