Labshake search
Citations for R&D Systems :
1801 - 1850 of 3688 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with BMP4 (30 ng/mL; R&D Systems), PD0325901 (100 nM ...
-
bioRxiv - Developmental Biology 2021Quote: ... ESCs were differentiated into anterior primitive streak through the addition of CDM2 basal medium supplemented with Activin A (30 ng/mL; R&D Systems), CHIR99021 (4 μM ...
-
bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
-
bioRxiv - Immunology 2020Quote: ... the ears of the mice were intradermally injected with 500 ng recombinant mouse IL-23 (R&D Systems cat. 1887-ML-010) dissolved in 25 μl PBS into one ear and 25 μl PBS into the contralateral ear ...
-
bioRxiv - Immunology 2021Quote: ... were measured by flow cytometry 2 days after activation with or without an extra stimulation with 25 ng/mL IL-2 (R&D Systems) during 15 min.
-
bioRxiv - Cell Biology 2021Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...
-
bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
-
bioRxiv - Molecular Biology 2022Quote: Human dermal fibroblasts transfected with either 10 nM si-circGLIS3 or scrambled siRNA (Eurofins Genomics, Ebersberg, Germany) for 48 hours and treated with 5 ng/ml TGF-β1 (R&D Systems) were rinsed and pelleted in PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
-
bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
-
bioRxiv - Immunology 2022Quote: ... Sorted cells were plated at 5000 cells per well in a 96 well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed and split into two new 48-well plate wells and cultured with IL-2 (5 ng/ml; R&D Systems), in the presence or the absence of CH-223191 (10 µM ...
-
bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (including B27 supplement with vitamin A) with 200 ng/ml rmWNT3A (R&D Systems, #1324-WN-010), 100 ng/ml rhFGF2 (R&D Systems ...
-
bioRxiv - Cell Biology 2022Quote: ... media was replaced with fresh media containing MCSF and receptor activator of nuclear κB (RANKL) (25 ng/mL each, R&D Systems), and media refreshed every 2-3 days thereafter until a total of seven days stimulation with RANKL ...
-
bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
-
bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
-
bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
-
bioRxiv - Immunology 2023Quote: ... Sorted cells were plated at 5000 cells per well in a 96-well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
-
bioRxiv - Developmental Biology 2023Quote: ... EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were seeded at 6x104 cells/cm2 and grown for 24 h until they reached 70-80% confluency and following treated with RANKL (30 ng/mL; 462-TR, R&D systems), with or without E2 (10-8 M ...
-
bioRxiv - Immunology 2022Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...
-
bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
-
bioRxiv - Microbiology 2023Quote: ... anti-hCD14 magnetic beads were used and isolated cells were differentiated to human monocyte-derived macrophages (hMDMs) by addition of 50 ng/ml rhM-CSF (R&D Systems) to XVivo medium (Lonza) ...
-
bioRxiv - Molecular Biology 2024Quote: ... containing 0.1% FBS (Hyclone Laboratories Inc.)] and stimulated with 10 ng/mL rat PDGF-AA ligand (R&D Systems, Minneapolis, MN, USA) diluted from a 1.5 μg/mL working solution in 40 nM HCl containing 0.1% BSA for the indicated length of time ...
-
bioRxiv - Cell Biology 2024Quote: ... It was counted as Day 0 when medium was changed to SF medium with 8 ng/mL recombinant Activin A (R&D Systems), 5 ng/mL recombinant human VEGF 165 Protein (R&D Systems ...
-
bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Cell Biology 2023Quote: ... after which the organoids were cultured for 13 days in E6 supplemented with 50 ng/mL bone morphogenetic protein-7 (BMP7, R&D systems) and 10 ng/mL human epidermal growth factor (hEGF ...
-
bioRxiv - Immunology 2023Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
-
bioRxiv - Immunology 2022Quote: ... 6.25 ng pMHC and 6.25 ng recombinant mouse PD-L1 with 6-His tag (R&D systems) in 2% BSA were injected into each well in this step ...
-
bioRxiv - Microbiology 2021Quote: ... surface blocking was conducted by incubated surfaces with anti-NgR1 antibody (R&D Systems; 100 µg/mL). Measurements were taken at a retraction velocity of 1 µm/s ...
-
bioRxiv - Immunology 2022Quote: ... 1% PS and 100 IU/ml of human recombinant IL-2 (R&D Systems, 202-IL-050). Cells were used at low passage numbers ...
-
Suppression of the Integrated Stress Response in Islet β Cells Decreases Risk of Autoimmune DiabetesbioRxiv - Cell Biology 2023Quote: ... the proinflammatory cytokine cocktail contained 1000 IU/mL human IFN-γ (R&D Systems; 285-IF-100) and 50 IU/mL human IL-1β (R&D Systems ...
-
bioRxiv - Immunology 2021Quote: ... were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT, R&D Systems, Minneapolis, MN, USA) diluted in 1× PBS pH 7.0 and incubated at 2-8°C overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
-
bioRxiv - Microbiology 2021Quote: ... the BMMs became adherent for the osteoclastic differentiation using a complete DMEM supplemented with 50 ng/ml of receptor activator of nuclear factor kappa-B ligand (RANKL, R&D System, USA) and 20 ng/ml of M-CSF (R&D System ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
-
bioRxiv - Pathology 2020Quote: ... were transduced into another line of HK-2 cells in the same manner as that of LOXL2 shRNA particles and were further incubated with media containing either TGF-β (20 ng/ml, R&D Systems) or vehicle (0.1% 4mM HCl/BSA ...
-
bioRxiv - Pathology 2020Quote: ... Cells were cultured to 70-80% confluence and then treated with vehicle (4mM HCl with 0.1% bovine serum albumin) or 10 ng/mL TGF-β1 (Cat#: 7754-BH-005, R&D Systems, Minneapolis, MN) or 10 ng/mL TNF-α (Cat#210-TA-020 ...
-
bioRxiv - Genetics 2020Quote: ... Cells were incubated in 0.5% serum-containing media before treatment with 200 ng/mL recombinant Shh N-terminal peptide (R&D Systems, 1314-SH) diluted in dimethyl formamide (DMF).
-
bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then seeded onto 24 well plates (106 cells/well) with or without 10 ng/mL murine IFN-γ (R&D Systems). Sixteen hours later ...
-
bioRxiv - Cell Biology 2022Quote: ... primary BMSCs were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 72 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD) and Wnt3a (100 ng/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... primary BMSCs isolated from C57BL/6 mice were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 48 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD), Wnt3a (100 ng/ml ...
-
bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL recombinant human basic FGF (#233-FB, R&D Systems, Germany) and 10 µM Y-27632 (only for the first 24 h after passaging) ...