Labshake search
Citations for R&D Systems :
1801 - 1850 of 2582 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175) antibody (catalog no. MAB835; R&D Systems, Minneapolis ...
-
bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
-
bioRxiv - Immunology 2021Quote: ... were coated with anti-IFN-γ antibody (capture mAb; 1 µg/mL) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Plates were blocked using culture medium (DMEM containing 10% FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40; AF3075; R&D Systems) and rabbit polyclonal antibodies against GFAP (1:250 ...
-
bioRxiv - Bioengineering 2020Quote: Surface expression of decoy constructs on engineered MSC lines was assessed by using either APC-conjugated rat-anti-mouse gp130 (IL6ST) antibodies (clone FAB4681A, R&D Systems) or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1 ...
-
bioRxiv - Physiology 2022Quote: ... incubated in primary antibodies (mouse monoclonal anti-HNF4α P1 and P2; catalog no. PP-K9218-00 and PP-H6939-00, respectively, R&D Systems) in 1% milk ...
-
bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems, catalog #: PS022), a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore ...
-
bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
-
bioRxiv - Genetics 2022Quote: ... Membranes were probed by incubation for 1 hour at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592 ...
-
bioRxiv - Immunology 2022Quote: ... 1:1000 diluted in PBS followed by a secondary fluorochrome anti-rabbit NorthernLights NL637-conjugated antibody (NL005; R&D systems, 1:200). Sections were then mounted with Fluormount G containing DAPI (Southern Biotech) ...
-
bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
-
bioRxiv - Neuroscience 2022Quote: ... The neurofilament marker SMI32 (mouse monoclonal, 1:1000; Sternberger Monoclonals) and a goat anti-osteopontin antibody (OPN, 1:1000; R&D Systems) were used to label alpha RGCs ...
-
bioRxiv - Immunology 2023Quote: ... 25 μl of the supernatant was diluted with 25 μl 1% BSA PBS-T and added to a Nunc Maxisorp plate coated with 1 μg/ml anti-C5a capture antibody (C5a DuoSet ELISA kit, R&D systems). Next ...
-
bioRxiv - Bioengineering 2023Quote: Cytokine profiling of the MBVs isolated from the aged and young mouse breast ECMs was performed using the dot blot-based Proteome Profiler Mouse XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated in the following primary antibodies overnight at 4L: IL-17B (1:100, Cat #1248, RRID:AB_354696, R&D Systems, Minneapolis, MN), TNF-alpha (1:100 Cat #AF-410 ...
-
bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
-
bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
-
bioRxiv - Systems Biology 2023Quote: ... primary RTK antibodies were used at a 1:1000 concentration followed by anti-rabbit or -mouse-HRP (R&D Systems #HAF008, #HAF007). Primary antibodies p-ErbB2 (MilliporeSigma #04-293 ...
-
bioRxiv - Bioengineering 2023Quote: ... with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556 ...
-
Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... TMA slides were stained at the histopathology core of Research Institute of McGill University Health Centre (RI-MUHC) using primary antibodies including anti-NE (MAB91671100; R&D Systems), anti-CD66b (392902 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
-
bioRxiv - Cancer Biology 2023Quote: ... It is worth noting that only the LRIG1 was incubated with a secondary antibody conjugated to allophycocyanin (APC; R&D Systems #F0127). LRIG1 cells were incubated with secondary antibody for 30 minutes in the dark on ice ...
-
bioRxiv - Developmental Biology 2022Quote: Cells were dissociated with TrypLE on day 6 and 8 of CMEC and PMEC protocol and stained with VE-Cadherin PE-conjugated Antibody (R&D Systems). Then VEC+ cells were sorted using FACSAria III ...
-
bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
-
bioRxiv - Immunology 2023Quote: ... followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (HAF107; R&D Systems). As a control ...
-
bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
-
bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Melanoma cells were then cultured with aged or young CM in the presence or absence of rIGFBP2 and/or neutralizing IGFBp2 antibody (AF674 from R&D systems). Wells were imaged using a Nikon TE2000 inverted microscope.
-
bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
-
bioRxiv - Cell Biology 2024Quote: Exogenous and endogenous GDNF in the medium was neutralized with anti-GDNF antibody (#AF-212-NA, R&D system, Minneapolis, MN, USA). 0.25 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
-
bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used in this study: Goat IgG against VE-cadherin (1:100, AF1002, R&D Systems, Minneapolis, MN), pY658 and pY685 VEC (1:40 ...
-
bioRxiv - Bioengineering 2024Quote: ... CAR T cells for the initial challenge day 0 imaging experiment were stained with the following antibody mix in FACS buffer (PBS 1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec ...
-
bioRxiv - Bioengineering 2024Quote: ... cells from re-challenge plates were stained with the following antibody mix in FACS buffer (PBS1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec) ...
-
Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... IGF-1 and VEGF-B in tissue homogenates and plasma samples were determined by double-antibody sandwich ELISA according to the manufacturer instructions (IGF-1, MG100 and VEGF-A, MMV00, R&D Systems, USA ...
-
bioRxiv - Neuroscience 2024Quote: ... cochlear frozen mid-modiolar cross sections or whole mounts were incubated overnight at room temperature with combinations of following primary antibodies: goat anti-CD45 (R&D Systems, catalog # AF114 ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 2 h at room temperature with secondary antibodies in TBS-T containing 10% milk: anti-Mouse IgG (HAF018, R&D Systems) and anti-Rabbit IgG (A9196 ...
-
bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
-
bioRxiv - Developmental Biology 2024Quote: ... of medium samples were analysed by SDS-PAGE under reducing conditions and underwent Western blotting using anti-Wnt5a antibody (MAB6452, R&D systems). Immune signals for exogenously added Wnt5a in the medium and cell lysate were quantified using ImageJ ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer and stained in 50 µL for flow cytometry using the following antibodies: anti-CCR2 (475301, R&D Systems), anti-CD11b (M1/70 ...
-
bioRxiv - Cell Biology 2022Quote: ... Some cells were treated 0.01µg/ml recombinant human WNT3A (R&D Systems) as positive control ...
-
bioRxiv - Genetics 2020Quote: Cells were washed with Flow Cytometry Staining Buffer (FC001, R&D Systems) and then stained in 50ul of Staining Buffer for one hour on ice with the following conjugated antibodies multiplexed at the indicated dilutions ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were treated with CHIR99021 (8 µM, R&D systems, #4423/10) in the STEMdiff APEL2 medium (Stem Cell Technologies ...