Labshake search
Citations for R&D Systems :
1751 - 1800 of 2902 citations for IL 9 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2019Quote: ... cells were simulated in R-10 medium in the presence of either 20 ng/mL IL-23 (R&D Systems) or 250 ng/mL PMA (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... A one-plex kit was used to measure the production of interleukin (IL)-22 (R&D Systems®, LXSAHM-01), and a four-plex was used to measure the production of IL-2 ...
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bioRxiv - Cell Biology 2020Quote: ... ELISA kits (CCL17, DY364, MCP1, DCP00, MDC, DMD00, IL-4, D4050, GM-CSF, DY215) were purchased from R&D Systems. PDGF-AA ELISA kits (EHPDGF ...
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bioRxiv - Immunology 2021Quote: ... and IL-13 cytokine levels in BALF were measured with the respective Sandwich-ELISA kits (R&D Systems, MN, US) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Immunology 2020Quote: Concentrations of IL-6 in cell culture supernatant were determined using the mouse DuoSet kit (R&D Systems, Minneapolis, MN). All assays were performed according the manufacturer’s supplied protocol ...
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bioRxiv - Immunology 2020Quote: Mice were intranasally challenged with Alternaria alternata extract (Greer, Lenoir, NC) or recombinant IL-33 (R&D Systems, Minneapolis, MN) diluted in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... NED] and 3U.ml−1 EPO [Roche]) supplemented with 1 ng.ml−1 IL→ 3 and 10 ng.ml−1 SCF (both R&D Systems) and 1 µg.ml−1 doxycycline for 4 days ...
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bioRxiv - Cell Biology 2022Quote: IL-1β in mice serum and cell-free supernatants were measured by Quantikine® sandwich ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Serum IL-6R levels were measured using an immunoassay method validated at QPS (Quantikine ELISA; R&D Systems Minneapolis, MN). Serum IL-6 and CRP levels were measured using in vitro diagnostic methods validated at PPD (Roche Cobas ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Microbiology 2023Quote: ... and the anti-inflammatory IL-10 ELISAs were performed using Quantikine® Colorimetric Sandwich ELISA Kits (R&D Systems, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were collected 24 h later and analyzed for IL-17A by DuoSet ELISA (R&D Systems, Minneapolis, USA). Concentrations of IL-17A in the used CM was 764 pg/ml ...
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bioRxiv - Physiology 2023Quote: ... and IL-6 levels were quantified in BALF and lung tissues using commercially available DuoSet ELISA kits (R&D Systems) according to manufacturer’s instructions and published protocols 22 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse IL-3 (403-ML) and 10 ng/mL mouse L-6 (406-ML) (R&D Systems). Total RNA was extracted using the RNeasy Mini kit (QIAGEN ...
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bioRxiv - Cancer Biology 2020Quote: Concentrations of murine IL-11 in the culture supernatants were determined by ELISA according to the manufacturers’ instruction (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
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bioRxiv - Systems Biology 2022Quote: ... Levels of lymphocyte-derived cytokines (IFNγ, IL-17) were determined after 7 days (PeliKine Compact or Duoset ELISA, R&D Systems). Baseline inflammatory plasma markers from both cohorts ...
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bioRxiv - Microbiology 2019Quote: ... and KC and IL-1β from mouse brain homogenates were detected by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Pathology 2020Quote: ... 4°C) and plasma was analyzed for TNF-α and IL-10 (porcine specific ELISA kit, R&D Systems, Abingdon, UK). For qPCR ...
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bioRxiv - Immunology 2019Quote: ... the cells were transferred to an IL-17A ELISPOT plate for an additional 24hours and then developed according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... The cytokine concentrations in the culture supernatant were determined using quantitative ELISA (Mouse TNF and IL-10 DuoSet; R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Released IL-8 was determined in supernatants collected at 24 hrs using an ELISA kit (Biosource Europe and R&D Systems), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml soluble anti-CD28 and cultured in the absence (Th0) or presence of 2.5 ng/ml IL-12 (R&D Systems) (Th1 ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: ... Sanofi-aventis Cat# NAC2004–5843-01) and interleukin-4 (IL-4; 1000 IU/mL; R&D Systems Cat# 204-1 L). Mature ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Mice were injected with 100 ng/mL IL-1β (R&D Systems, #401-ML-005, 100 µg/mL stock in PBS) and 50 ng/mL TNF (R&D Systems ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-10 and CCL7 were measured using a multiplex Luminex bead assay by R&D Systems (R&D Systems, Minneapolis, MN). CCL2 and TL1A were measured using human CCL2 (MCP-1 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at 1.5×105/ml in primary differentiation medium supplemented with 1ng/ml interleukin-3 (IL-3, R&D Systems), 10ng/ml SCF and 1μg/ml doxycycline ...
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bioRxiv - Neuroscience 2022Quote: ... The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) ...
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bioRxiv - Immunology 2023Quote: ... A microtiter plates with 96 well were incubated with anti-TNF-α or anti-IL-1β primary antibodies (R&D Systems) diluted in PBS (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
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bioRxiv - Cell Biology 2024Quote: ... IL-1β and TNFα in plasma were measured using ELISA kits (DY410-05, DY406-05, DY401-05, R&D Systems, MN) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... and human recombinant bFGF and EGF (25 ng/ml each; R&D Systems), hereafter ...
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bioRxiv - Genetics 2021Quote: ... Stimulation was performed using 10 ng/ml recombinant human TGFβ1 (R&D system). C646 dissolved in DMSO was treated at a dose of 20 μM for 24 hours of pretreatment before TGFβ1 stimulation ...
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bioRxiv - Microbiology 2021Quote: ... IFNβ was quantified using human IFN-beta Quantikine ELISA kit (R&D Systems). All measurements were made following the manufacturers’ protocols.
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bioRxiv - Immunology 2022Quote: IFN-β ELISAs were performed using Human IFN-beta DuoSet (R&D Systems) with supernatants collected from cultured cells.
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bioRxiv - Cell Biology 2022Quote: ... 1.5 μg/mL recombinant human ICAM-1/CD54 Fc chimera (R&D systems) at 4° C ...
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bioRxiv - Immunology 2022Quote: ... or 1 μg/ml goat IgG anti-human TIM-3 (R&D systems) and alone or in combination of 5 mM Metformin (SIGMA) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human His-tagged CD80 (R&D Systems 9050-B1, Minneapolis, MN, USA) or CD86 (R&D Systems 9090-B2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 µM recombinant yeast or human GST-Uba1/UBE1 (E1, R&D Systems), 2.5 µM recombinant yeast or human ubiquitin (R&D Systems) ...