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1701 - 1750 of 2904 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020

• Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein

  Christopher Cyrus Kuhn, et al., bioRxiv - Cell Biology 2022

  Quote: ... or ECM proteins fibronectin (R&D systems #3420-001-01) and collagen-I (Chrono-Log #385 ...
• Therapeutic immune cell engineering with an mRNA : AAV-Sleeping Beauty composite system

  Lupeng Ye, et al., bioRxiv - Bioengineering 2023

  Quote: ... Cells were incubated with CD22-Fc protein (R&D Systems) in PBS for 30 min on ice and washed with PBS twice to remove the unbonded protein ...
• Defective mesenchymal Bmpr1a-mediated BMP signaling causes congenital pulmonary cysts

  Yongfeng Luo, et al., bioRxiv - Developmental Biology 2024

  Quote: ... BMP4 protein was purchased from R&D Systems (5020-BP). LDN193189 was provided by Dr ...
• RNA 5ʹ terminal nucleotide determines the strength of the RIG-I/IFN signaling pathway

  Magdalena Wolczyk, et al., bioRxiv - Molecular Biology 2023

  Quote: ... A standard curve was generated in parallel by serial dilutions of recombinant IFN-β standard in DMEM (R&D Systems #8499-IF-010 and #8234-MB-010). After overnight incubation (24 h) ...
• The lncRNA Malat1 Inhibits miR-15/16 to Enhance Cytotoxic T Cell Activation and Memory Cell Formation

  Benjamin D Wheeler, et al., bioRxiv - Immunology 2023

  Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
• IgG derived dendritic cells can induce production of IL-17 by T cells in multiple sclerosis

  Nazanin Pournasrolla, et al., bioRxiv - Immunology 2022

  Quote: ... immobilized hIgG, or 50 ng/ml recombinant GM-CSF (rGM-CSF) (Bioscience, US) plus 20 ng/ml IL-4 (R&D systems Cat.No. 204-IL-010, US), for 5 days to obtain immature dendritic cells (iDC) ...
• Proinflammatory cytokine-induced alpha-cell impairment in human islet microtissues is partially restored by dual incretin receptor agonism

  Kristine Henriksen, et al., bioRxiv - Cell Biology 2024

  Quote: Islet MTs were cultured for 1 day (short-term) or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
• Innate immune control of influenza virus interspecies adaptation

  Parker J. Denz, et al., bioRxiv - Microbiology 2023

  Quote: IL-6 ELISAs were performed on supernatant from mouse lung homogenates using the Mouse IL-6 Duoset ELISA kit from R&D Systems (catalog # DY406) according to manufacturer’s instructions.
• Targeting NGF but not VEGF or BDNF signaling reduces endometriosis-associated pain in mice

  Tiago H. Zaninelli, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... The measurement of VEGF from mouse endometriosis lesions was performed according to manufacturer’s instructions using mouse VEGF quantikine ELISA Kit (Cat# MMV00, R&D systems, Minneapolis, MN, USA).
• Template-Directed RIG-I Agonist Assembly for Targeted Cancer Immunotherapy

  Subrata K. Ghosh, et al., bioRxiv - Bioengineering 2023

  Quote: ... IP-10/CXCL10 release from mouse cells was assayed using the Quantikine Mouse IP-10 ELISA assay (R&D Systems, Catalog No. DY466-05) using cell-free supernatants of stimulated cells according to the manufacturer’s instructions ...
• Intranasal VLP-RBD vaccine adjuvanted with BECC470 confers immunity against Delta SARS-CoV-2 challenge in K18-hACE2-mice

  Katherine S Lee, et al., bioRxiv - Immunology 2023

  Quote: Concentrations of CXCL10 in K18-hACE2 mice were measured in serum and lung supernatant collected at euthanasia using the Mouse Magnetic Luminex Assay kit for mouse CXCL10/IP-10/CRG2 (R&D Systems LXSAMSM-01). Both serum and supernatant samples were diluted 1:2 in the kit’s Calibrator Diluent RD6-52 then utilized in the assay procedure provided by the manufacturer ...
• Hypothalamus-specific NSCs derived from hPSCs ameliorate age-associated dysfunction upon transplantation into aged mouse hypothalamus

  Yanuar Alan Sulistio, et al., bioRxiv - Cell Biology 2024

  Quote: ... Serum levels of cytokines and leptin were also determined using a Proteome Profiler Mouse XL Cytokine Array and a Mouse/Rat Leptin Quantikine ELISA Kit (R&D Systems, Minneapolis, Minnesota), respectively ...
• Defective endosome-TGN retrograde transport promotes NLRP3 inflammasome activation

  Zhirong Zhang, et al., bioRxiv - Immunology 2021

  Quote: ... or Mouse TNF-alpha DuoSet ELISA (DY410, R&D Systems), separately ...
• Transcriptional Reprogramming of Skeletal Muscle Stem Cells by the Niche Environment

  Felicia Lazure, et al., bioRxiv - Genetics 2021

  Quote: ... Antibodies for anti-mouse ITGA7-AF647 (R&D systems, FAB3518R), anti-mouse CD31-PE (Invitrogen 12-0311-81) ...
• Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epithelium

  Iulia Rusu, et al., bioRxiv - Immunology 2021

  Quote: ... and mouse LTBR-Fc were purchased from R&D systems.
• Hematopoietic Stem and Progenitor Cell Aging is Initiated at Middle Age Through Decline in Local Insulin-Like Growth Factor 1 (IGF1)

  Kira Young, et al., bioRxiv - Cell Biology 2020

  Quote: ... A mouse-specific IGF1 Immunoassay kit (ELISA, R&D Systems) and a mouse-specific IGF2 PicoKine kit (ELISA ...
• Statins attenuate antiviral IFN-β and ISG expression via inhibiting IRF3/JAK/STAT signaling in poly(I:C)-treated hyperlipidemic mice and macrophages

  Atsushi Koike, Kaito Tsujinaka, Ko Fujimori, bioRxiv - Biochemistry 2020

  Quote: ... and Mouse TNF-alpha Quantikine ELISA kit (R&D Systems), respectively ...
• PD-1-induced T cell exhaustion is controlled by a Drp1-dependent mechanism

  Luca Simula, et al., bioRxiv - Immunology 2021

  Quote: ... 20ng/ml mouse IL-7 (R&D System 407-ML) and 20ng/ml mouse IL-15 (R&D System 447-ML) ...
• Toll-like receptor 5 as a novel receptor for fungal zymosan

  Stefanie Reuter, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-TLR5-Fc mouse was purchased from R&D Systems, Inc.
• Inactive variants of death receptor p75^NTR reduce Alzheimer’s neuropathology by interfering with APP internalization

  Chenju Yi, et al., bioRxiv - Neuroscience 2020

  Quote: ... and 1μg/mL mouse Cultrex® Laminin (R&D Systems). Cultured hippocampal neurons were maintained in serum-free defined Neurobasal media supplemented with B27 (Invitrogen) ...
• BSG/CD147 and ACE2 receptors facilitate SARS-CoV-2 infection of human iPS cell-derived kidney podocytes

  Titilola D. Kalejaiye, et al., bioRxiv - Bioengineering 2021

  Quote: ... Human/Mouse/Rat/Hamster ACE-2 (R&D systems, AF933), Human TRA-1-85/CD147 (R&D systems ...
• A systems biology analysis of adrenergically stimulated adiponectin exocytosis in white adipocytes

  William Lövfors, et al., bioRxiv - Systems Biology 2020

  Quote: ... Secreted adiponectin (measured with mouse ELISA DuoSets; R&D Systems) was analyzed in relation to total protein content (Bradford protein assay) ...
• Exploration of BAY 11-7082 as a novel antibiotic

  Victoria E. Coles, et al., bioRxiv - Microbiology 2021

  Quote: ... (DuoSet ELISA, Mouse 1L-1β/IL-1F2, R&D Systems), as previously described.58 Media was diluted in a 1:10 ratio in ELISA reagent dilute (1% BSA in PBS ...
• Spatial and functional links between cellular virological state and progression of liver fibrosis in chronic hepatitis B

  Xiaonan Zhang, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse anti-HNF4a (H1415) was purchased from R&D systems. Rabbit monoclonal antibody to COX IV (#4844 ...
• Conserved Transcription Factors Control Chromatin Accessibility and Gene Expression to Maintain Cell Fate Stability and Restrict Reprogramming of Differentiated Cells

  Maria A. Missinato, et al., bioRxiv - Cell Biology 2021

  Quote: ... and mouse anti-TUJ1 (R&D Systems, MAB1195, 1:200); rabbit anti-NANOG (Abcam ...
• Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain

  Maria Kowalski-Jahn, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-Nluc (R&D systems, MAB100261-SP, mouse, 1:500) and GAPDH (Cell Signaling Technology ...
• Antisense oligonucleotide strategies to increase progranulin levels for frontotemporal dementia

  Geetika Aggarwal, et al., bioRxiv - Neuroscience 2022

  Quote: ... and anti-mouse progranulin polyclonal antibody (R&D Systems, AF2557), and an anti-α-tubulin monoclonal antibody (Sigma ...
• NF-κB memory coordinates transcriptional responses to dynamic inflammatory stimuli

  Andrew G. Wang, et al., bioRxiv - Systems Biology 2022

  Quote: ... mouse interleukin 1 beta (IL-1β; R&D Systems, 401ML010CF), ultrapure lipopolysaccharide (LPS ...
• Reactive astrocytes transduce blood-brain barrier dysfunction through a TNFα-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

  Hyosung Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-Glut1 (1:1000 dilution, FAB1418G, R&D systems), mouse anti-occludin (1:500 dilution ...
• Arginine 65 methylation of Neurogenin 3 by PRMT1 is a prerequisite for development of hESCs into pancreatic endocrine cells

  Gahyang Cho, et al., bioRxiv - Developmental Biology 2022

  Quote: ... mouse anti-pancreatic polypeptide (PPY, 1:200, R&D Systems), and rabbit anti-somatostatin (SST ...
• Cochlear implant material effects on inflammatory cell function and foreign body response

  Megan J. Jensen, et al., bioRxiv - Bioengineering 2022

  Quote: ... The Mouse Cytokine Array Kit was from R&D Systems. The Cochlear Practice Electrodes (Slim Straight electrodes ...
• Surf4 Promotes Endoplasmic Reticulum Exit of the Lysosomal Prosaposin-Progranulin Complex

  S. Devireddy, S.M. Ferguson, bioRxiv - Cell Biology 2021

  Quote: ... sheep anti-mouse progranulin (R&D systems; Catalog no # AF2557), mouse anti-LAMP1 (DSHB ...
• SARS-COV-2 induced Diarrhea is inflammatory, Ca²⁺ Dependent and involves activation of calcium activated Cl channels

  Mark Donowitz, et al., bioRxiv - Physiology 2021

  Quote: ... anti-ACE2 – monoclonal mouse IgG2A (R&D systems catalog # MAB933); Phalloidin and secondary fluorescent AlexaFluor antibodies from Invitrogen ...
• Engineering of extracellular vesicles for display of protein biotherapeutics

  Dhanu Gupta, et al., bioRxiv - Bioengineering 2020

  Quote: ... and APC-labelled anti-mouse gp130 antibodies (R&D Systems, cat #FAB4681A ...
• Plasma iron controls neutrophil production and function

  Joe N. Frost, et al., bioRxiv - Immunology 2022

  Quote: ... Mouse TNF-alpha DuoSet Elisa (R&D Systems, catalog MTA00B) were used to detect the levels of CCL2 and TNF-alpha ...
• Plasma iron controls neutrophil production and function

  Joe N. Frost, et al., bioRxiv - Immunology 2022

  Quote: ... Mouse CCL2 DuoSet Elisa (R&D Systems, catalog# DY479-05), Mouse TNF-alpha DuoSet Elisa (R&D Systems ...
• Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog Pathway Inhibitor-1

  Meropi Bagka, et al., bioRxiv - Cell Biology 2022

  Quote: ... 1:500 Goat anti-mouse GLI3 (R&D systems, AF3690)) overnight at 4 C ...
• Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog Pathway Inhibitor-1

  Meropi Bagka, et al., bioRxiv - Cell Biology 2022

  Quote: ... 1:500 Goat anti-mouse GLI2 (R&D systems, AF3635), 1:500 Goat anti-mouse GLI3 (R&D systems ...
• ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

  Ken Shirato, Jun Takanari, Takako Kizaki, bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... and Quantikine Mouse IL-1β ELISA Kit (R&D Systems), as described previously [14 ...
• Endothelial cells promote productive HIV infection of resting CD4+ T cells by an integrin-mediated cell adhesion-dependent mechanism

  Catherine M. Card, et al., bioRxiv - Immunology 2020

  Quote: ... or mouse IgG1κ isotype control (clone 11711, R&D Systems). Wells were coated with 5μg/ml of either rhICAM/Fc chimera (R&D Systems ...
• Molecular and genetic regulation of pig pancreatic islet cell development

  Seokho Kim, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-human NKX6.1 (R&D systems, AF5857, 1:200,) rabbit anti-MAFB (Bethyl ...
• Endothelial cells elicit a pro-inflammatory response to SARS-CoV-2 without productive viral infection

  Lilian Schimmel, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-ICAM-1 (R&D systems, BBA3, IF1:200), mouse anti-ICAM-1 (Santa Cruz Biotechnology ...
• Intertumoral Genetic Heterogeneity Generates Distinct Tumor Microenvironments in a Novel Murine Synchronous Melanoma Model

  Shuyang S. Qin, et al., bioRxiv - Cancer Biology 2020

  Quote: ... or 100ng/mL of mouse IFN-γ (R&D Systems) in regular culture media ...
• Diacylglycerol kinase ζ deficiency triggers early signs of aplastic anemia in mice

  M Martín-Salgado, et al., bioRxiv - Immunology 2020

  Quote: ... incubated with rat anti-mouse S1P1-APC (R&D Systems) for 1 h on ice and washed once ...
• ETV2 primes hematoendothelial gene enhancers prior to hematoendothelial fate commitment

  Jeffrey D. Steimle, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 5 ng/ml mouse VEGF (R&D systems Cat. 494MV). For mesoderm induction in the AB regimen ...
• Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD

  Jaspreet K. Osan, et al., bioRxiv - Microbiology 2020

  Quote: ... and anti-ACE2 mouse monoclonal (1:20) (R&D Systems). The next day ...
• Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities

  Esther Redder, et al., bioRxiv - Cell Biology 2021

  Quote: ... goat polyclonal anti-mouse VEGFR-3 (R&D Systems, AF743) and rabbit polyclonal anti-RFP (Rockland ...
• Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities

  Esther Redder, et al., bioRxiv - Cell Biology 2021

  Quote: ... rat monoclonal anti-mouse Lyve1 (clone 223322, R&D Systems), goat polyclonal anti-mouse VEGFR-3 (R&D Systems ...
• Regulatory T cell activation triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses

  Gang Wu, et al., bioRxiv - Immunology 2020

  Quote: ... Polyclonal goat anti-mouse CD48 was from R&D systems, Abingdon ...
• Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19

  Tsun-Yung Kuo, et al., bioRxiv - Immunology 2020

  Quote: ... and Mouse IL-5 Quantikine ELISA Kit (R&D System). The OD450 values were read by Multiskan GO (ThermoFisher).