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Citations for R&D Systems :
1651 - 1700 of 4972 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and human total MMP-3 (R&D Systems, cat.# DY513) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... human GDF-15 Duo Set (R&D Systems, cat.# DY957), human Serpin E1/PAI-1 Duo Set (R&D Systems ...
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bioRxiv - Biochemistry 2024Quote: ... 15 μg of human N1 antibody (AF5317: R&D Systems) was covalently coupled to 60 μl of Protein G-Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... or Human IFN-γ DuoSet ELISA kit (R&D Systems). Supernatant of co-cultures is harvested at indicated times and stored at -20°C until analyzed.
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bioRxiv - Cell Biology 2024Quote: ... the human GDF-15 ELISA Kit by R&D Systems was used ...
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bioRxiv - Neuroscience 2024Quote: ... and control human IgG-Fc (110-HG, R&D Systems) were performed in NSC-34 cells (CELLutions Biosystems ...
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bioRxiv - Biophysics 2024Quote: ... Human MGF-E8 protein (R&D Systems, 2767-MF-050) and human protein-S (Coachrom ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Soluble Receptor Array (R&D Systems, Minneapolis, MN, # ARY012) was performed according to a manufacturer protocol ...
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bioRxiv - Physiology 2021Quote: ... either in the absence or presence of increasing concentrations of recombinant NMB (0.1-100 nM R&D systems GmBH) 500 μM carbachol,a cholinomimetic agonist ...
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bioRxiv - Neuroscience 2021Quote: ... except for pre-crossing cultures where the medium was supplemented with recombinant Netrin (50 ng/ml, R&D Systems). Primary neurons were plated at low density (8’000-10’000 cells/well ...
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bioRxiv - Biophysics 2022Quote: ... The medium was supplemented with 10 ng/ml recombinant mouse IL-2 (rmIL-2) (402-ML, R&D systems) for long-term culture of mouse ILC2s.
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bioRxiv - Molecular Biology 2019Quote: ... were pre-treated with 2ug/mL recombinant mouse Slit2 protein (gln26-gln900) (rSlit2) (R&D system, 5444-SL-050) or PBS for at least 18 hours before removing inserts ...
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bioRxiv - Immunology 2020Quote: ... 2-incubation with 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) in microscopy buffer for 1 h at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Explants were either treated with recombinant BMP2/7 heterodimers (32 nM, R&D Systems, Cat No. 3229-BM-010) or Noggin (300 ng/μl ...
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bioRxiv - Immunology 2022Quote: ... PPS14-TT together with recombinant IL-6 (500 ng/adult and 100 ng/neonate (R&D systems, Minneapolis, MN) was emulsified with aluminum hydroxide ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were then fasted for 16h overnight before intraperitoneal administration of recombinant mouse leptin (3μg/g) (R&D System) to half of the cohort and vehicle control (sterile Tris-HCl 20 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR-608 050/CF, Minneapolis MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466-609 NA ...
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bioRxiv - Microbiology 2023Quote: ... suum were incubated with or without recombinant acidic mammalian chitinase (AMCase, 9859-GH-050, R&D systems, Minneapolis, MN) at 4 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Developmental Biology 2022Quote: ... beads were soaked in 2uL of a 50:50 mix of recombinant WNT5A (R&D Systems: 645-WN-010) at stock concentration of 1ug/uL and PBS with 50ug/uL BSA ...
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bioRxiv - Cell Biology 2023Quote: ... starved for 2 hours and treated with 10 ng/ml rat recombinant EGF (R&D Systems, Minneapolis, MN, USA) for 5 min ...
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bioRxiv - Genetics 2023Quote: ... or vehicle for 24 hours followed by 8 hours of recombinant TNFα (2ng/ml, R&D Systems, Minneapolis MN). Similar experiments were conducted with pre-treatment of cells for 2 hours with Pyrrolidine Dithiocarbamate (PDTC ...
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bioRxiv - Neuroscience 2023Quote: ... NSCs were treated in the presence or absence of the recombinant proteins RSPO1 (7150-RS/CF, R&D Systems), RSPO2 (6946-RS/CF ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μL of sample containing either LNCaP crude extract or recombinant HK2 protein (R&D Systems™, Cat# 8179HK020) was added to a well of 96-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... specifically: goat anti-mouse platelet-endothelial cell adhesion molecule-1 (PECAM-1)/CD31 (R&D Systems), mouse anti-mouse α-smooth muscle actin (αSMA ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells in PBS were mixed 1:1 with Cultrex TM (R&D systems, Minneapolis, MN, USA) at a final concentration of 1 x 106 in 100 μl prior to injection ...
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bioRxiv - Cell Biology 2020Quote: ... dPSCs were differentiated into each of the three germ layers according to the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems®, Catalog # SC027B).
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
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bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (Cell Signaling; #5679S, western blot dilution 1:1000, IHC dilution 1:100; R&D Systems; AF2517, IF dilution 1:100), NR5A2 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor cells were resuspended in 1x PBS and mixed with methylcellulose (1:1 ratio) (R&D Systems). 8- to 10-week old naïve C57BL/6 mice were anesthetized with isoflurane and placed in a stereotactic frame ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-CD31/platelet-endothelial cell adhesion molecule-1 (PECAM-1) antibody (R&D Systems, AF3628,1:500), and rabbit anti-glial-fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2021Quote: ... They were then incubated with the noted concentrations of recombinant mouse (rm) Chi3l1 or IFNγ (R&D Systems, Minneapolis, MN) or CAY10471 (Cayman Chemical ...
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bioRxiv - Immunology 2019Quote: ... cover glasses were coated for 2 h at 37°C with 100 nM recombinant ICAM1-FC (796IC, R&D Systems) and washed 2 times with PBS ...
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bioRxiv - Cell Biology 2021Quote: Treatments were done 48 h after transfection using the following agents: recombinant WNT-3A (R&D systems, 5036-WN-010), recombinant WNT-5A (R&D systems ...
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bioRxiv - Immunology 2020Quote: Mice were intranasally challenged with Alternaria alternata extract (Greer, Lenoir, NC) or recombinant IL-33 (R&D Systems, Minneapolis, MN) diluted in PBS ...
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bioRxiv - Immunology 2022Quote: ... recombinant SARS-CoV-2 Spike RBD and recombinant SARS-CoV-2 Spike RBD B.1.617.2 L452R T478K were purchased from R&D systems. Proteins were reconstituted as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... DIV12 hippocampal neurons were treated with either 2 ug/mL of recombinant Nptx2 (CF 7816-NP-050, R&D Systems) or BSA as control ...
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bioRxiv - Immunology 2023Quote: ... BMDM were then treated with vehicle or recombinant mouse IFN-γ at 6.25 ng/ml (485-MI, R&D Systems) overnight and infected with Mtb Erdman strain at a multiplicity of infection of 5 in DMEM supplemented with 5% horse serum and 5% FBS ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... IFN-γ concentrations in cell-free supernatants were determined by Human IFN-γ DuoSet enzyme-linked immunosorbent assay (ELISA; DY285; R&D systems, Minneapolis, MN, USA). EYFP−/EBFP+ dLP-CHO cells with no anti-hPD1 payload circuit were used as the control cells.