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Citations for R&D Systems :
1651 - 1700 of 3738 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The samples were quantified by BCA assay and further equal protein was assayed by enzyme-linked immunosorbent assay (ELISA) for TNF-a using the manufacturer’s protocol (R&D systems, USA).
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bioRxiv - Neuroscience 2024Quote: ... the supernatant was collected and used to measure inflammatory mediators using the DuoSet enzyme-limited immunosorbent assay (ELISA) system (R&D Systems). The concentrations of CXCL1 ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... IGF-1 and VEGF-B in tissue homogenates and plasma samples were determined by double-antibody sandwich ELISA according to the manufacturer instructions (IGF-1, MG100 and VEGF-A, MMV00, R&D Systems, USA ...
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bioRxiv - Immunology 2024Quote: ... Total THP levels of a subset of urine samples (diluted 1:10) was determined by ELISA (catalog no. DY5144-05, R&D Systems) per manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Circulating CCL5 levels were measured in serum from CCR5+/+ mice treated with vehicle or aldosterone via Enzyme-Linked Immunosorbent Assay (ELISA) (R&D System).
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bioRxiv - Immunology 2024Quote: ... or 100 ng/ml N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cysteinyl-[S]-seryl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine (Pam3CSK4) for 6 h and IL6 secretion measured by ELISA (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... Cytokines in the supernatant were determined by fluorescent assays (Ladinig et al., 2014) or a porcine TNF-α ELISA (R&D Systems).
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bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
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Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 24 and 48 hours intervals from 3 BALB/C mice carrying RCC tumours receiving 1×107 PODS-IL-2 treatment following the final dose and tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Mice were sacrificed 24h post-infection and n=7 were analysed for bacterial load and IL-6 and KC using ELISA (R&D systems). A further n=3 animals were analysed using histology ...
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bioRxiv - Microbiology 2024Quote: ... interleukin 8 (IL-8) secretion was assessed with an Enzyme-Linked Immunosorbent Assay (ELISA) following the manufacturer’s protocol ((R&D system #DY535). The coat was made with 1:180 dilution of 360 μg/ml IL-8 capture antibody in PBS 1x ...
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bioRxiv - Microbiology 2024Quote: ... Cell culture supernatants were also collected from 96-well plate infections at 9 hpi and secreted IL-1β measured by DuoSet© Human IL-1β/IL-1F2 ELISA as per manufacturer’s instructions (R&D Systems). A core genome SNP ML phylogeny was constructed on the Australian ST10 lineage to demonstrate the genetic relationship of representative ST10 isolates that underwent in vitro phenotypic assays.
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bioRxiv - Cancer Biology 2024Quote: Cells were grown for 72 hours after which culture media was collected and immediately assayed for IL-6 levels (Human IL-6 Quantikine Elisa, R&D Systems). Optical density at 540nm was determined using a SPECTROstar Omega (BMG Labtech) ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Immunology 2023Quote: ... naïve B cells or CH12F3 cells were treated with 10ng/ml F(ab’)2 Fragment Goat Anti-Mouse IgM or 10ng/ml mIL-5 (R&D Systems) for 5 or 10 min and then collected for Western blotting or RNA extraction.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with PBS and incubated with an anti-human CCR6 mouse monoclonal antibody (1:50, R&D Systems, U.S.A.) and an anti-CD4 rabbit monoclonal antibody (1:30 ...
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bioRxiv - Neuroscience 2021Quote: ... for mouse samples or mouse anti-CD31 antibody (1:200, #BBA7, R&D systems, Minneapolis, MN, USA) for human samples ...
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bioRxiv - Pathology 2023Quote: ... Cytokine profiling of mouse lung homogenates was performed using a mouse proteomic cytokine profiler (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Mouse anti-tenascin-R 619 (MAB1624) and mouse anti-aggrecan (MAB11304) were purchased from R&D systems. Rabbit anti-aggrecan (AB1031 ...
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bioRxiv - Immunology 2022Quote: The cytokine/chemokine profile of mice plasma or culture supernatants was determined by using proteome profiler mouse cytokine array kit (R&D Systems, CAT. No. ARY006). The expression levels of cytokine/chemokine were measured by pixel density of each dot using the ImageJ software ...
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bioRxiv - Cell Biology 2022Quote: Intracellular cAMP expression levels were measured after 10min to 48h of PTH treatment using Mouse/Rat cAMP Assay Parameter™ Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions in both pre-differentiation COBs and BMSCs ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... IL-8 and TNF-α were quantified in the supernatants from uninfected and SARS-CoV-2-infected hiPSC-CMs by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Microbiology 2021Quote: CCL22/Ccl22 ELISAs were performed on culture supernatants (undiluted or diluted when necessary) using Immulon 4HBX flat bottom microtiter plates with the human CCL22/MDC DuoSet ELISA (R&D Systems DY336) or the mouse Ccl22/Mdc DuoSet ELISA (R&D Systems DY439 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in BALF were quantified by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol (DY406, DY410 and DY401 from R&D systems; Minnesota, USA). The UV absorbance at 450 nm and 570 nm were measured for the primary signal and for plate correction ...
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bioRxiv - Physiology 2021Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and Ccl19/Ccl21 levels were determined by indirect sandwich of enzyme-linked immunosorbent assay (ELISA) following manufacturer’s instructions (R&D Systems, Minneapolis, MN). Multi-analytic profiling beads using Milliplex® Multiplex mouse cytokine/chemokine panel I kit (EMD Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Doses were previously optimized and mice were intravenously injected with 5×1010 genome copies of both Control- and TSLP-AAV for 14 days with serum TSLP levels confirmed using a murine specific ELISA (R&D Systems MTLP00)4.
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bioRxiv - Cell Biology 2023Quote: ... plasma and tissues of interest were collected at different post-infection times and assessed by ELISA using commercially available antibodies and according to the procedures supplied by the manufacturer (R&D Systems, Minneapolis). All samples were stored at -20°C until processing ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 was measured by ELISA (enzyme-linked immunosorbent assay) with the obtained supernatant and analyzed according to the manufacturer’s protocol (R&D Systems, Minnesota, USA).
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bioRxiv - Bioengineering 2022Quote: ... The concentration of hCG-β in the culture medium was measured using the Human CG beta (HCG beta) Duoset ELISA (#DY9034-05, R&D Systems) according to the manufacturer protocol.
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bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected at indicated times and protein concentrations were determined by ELISA following the manufacturer’s instructions for IL-6 (R&D systems, DY406-05), TNFα (R&D systems ...
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bioRxiv - Cancer Biology 2022Quote: Medium from cells was harvested and used in the Human Cytokine Array Kit (R&D Systems), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... nitrite concentrations in cell culture supernatant were determined via Griess reaction assay kit (R&D Systems).
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bioRxiv - Bioengineering 2023Quote: P4 MSCs were assessed using the Human Mesenchymal Stem Cell Functional Identification Kit (R&D System) following the product instruction ...
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bioRxiv - Bioengineering 2023Quote: P4 MSCs were characterized with the Human Mesenchymal Stem Cell Verification Flow Kit (R&D Systems), including antibodies for positive markers CD90 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed by lysis buffer provided in the kit (R&D Systems, #895943). The supernatant was collected after centrifugation at 14,000xg for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were processed with a commercial DAB cell and tissue staining kit (#CTS019, R&D Systems). Primary antibody immunostaining was optimised for polyclonal rabbit anti-human peroxidasin (#abx101905 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl plasma from each mouse was tested using the mouse CXCL1/KC duo-set (R&D Systems). The experiments were done following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... A time course was performed on cells treated with 300 ng/mL of recombinant mouse VEGF-B186 (R&D Systems #767-VE-010) for 0 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...