Labshake search
Citations for R&D Systems :
1651 - 1700 of 3353 citations for Mouse 14 3 3 protein sigma SFN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... crassiceps excretory/secretory (E/S) product using commercially available ELISA reagents according to the manufacturer’s instructions (R&D Systems, Germany). 96 well plates were coated with 50 μl antibody solution (IL-1β ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
-
bioRxiv - Immunology 2022Quote: ... the conditioned medium was collected and TNF-α was quantified by enzyme-linked immunosorbent (ELISA) assay from R&D Systems according to the instructions of the manufacturer.
-
bioRxiv - Immunology 2022Quote: ... PTX3 and P-selectin were determined by enzyme-linked immunosorbent assay (DuoSet ELISA, R&D Systems and Cloud-Clone corp) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
-
bioRxiv - Cell Biology 2019Quote: Cytokine (IL6 and CXCL8) levels in tissue culture supernatants were determined by ELISA assay (DY206 and DY208; R&D Systems). All assays were performed according to the manufacturer’s instructions and read on a CLARIOstar microplate reader (BMG Labtech) ...
-
bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
-
bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: Serum IL-6R levels were measured using an immunoassay method validated at QPS (Quantikine ELISA; R&D Systems Minneapolis, MN). Serum IL-6 and CRP levels were measured using in vitro diagnostic methods validated at PPD (Roche Cobas ...
-
bioRxiv - Microbiology 2023Quote: The levels of secreted IFN-β were measured by an IFN-β enzyme-linked immunosorbent assay (ELISA) (R&D Systems) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were collected 24 h later and analyzed for IL-17A by DuoSet ELISA (R&D Systems, Minneapolis, USA). Concentrations of IL-17A in the used CM was 764 pg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
-
bioRxiv - Genetics 2023Quote: ... and vascular endothelial growth factor (VEGF) by sandwich ELISA using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
-
bioRxiv - Immunology 2024Quote: The chemokine CXCL13 was measured in LO chip effluent using a human CXCL13 ELISA assay (Quantikine, R&D Systems #DY801), following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2024Quote: ... Concentration of PCSK9 in the plasma and tissue samples were assessed by ELISA (Quantikine MPC900, R&D Systems, Minneapolis, MN). Protein extracts were obtained after lysis of tissues in 350-μL Tris HCl buffer pH=9.5 (10 mM ...
-
bioRxiv - Neuroscience 2024Quote: ELISAs were used to quantify CTSB and CTSS levels in CSF (both R&D DuoSets, R&D Systems, Minneapolis, USA). CSF samples were diluted 20x with reagent diluent prior to ELISA analysis ...
-
bioRxiv - Bioengineering 2024Quote: ... The DuoSet ELISA systems were used according to the manufacturer’s instructions (DY201, DY206, DY210, DY1607, DY217B, R&D Systems, respectively). Absorbance values were measured on Infinite® 200 Pro plate reader (Tecan ...
-
bioRxiv - Pathology 2019Quote: Analysis of the cytokine proteome was completed using a Mouse XL Cytokine Array Kit (R&D Systems, Bio-Techne Corporation, MN, USA). Gingiva and alveolar bone samples were individually pooled ...
-
bioRxiv - Immunology 2022Quote: CD4+ splenic T-lymphocytes were polarized to TH17 ex vivo by use of CellXVivo Mouse Th17 Cell Differentiation Kit (#CDK017, R&D Systems). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: Cytokine array was carried out in the young and aged L-MSC culture media using Proteome Profiler Mouse XL Cytokine Array kit (R&D Systems) as per manufacturer ‘s instructions.
-
bioRxiv - Cancer Biology 2022Quote: Human cytokine secretion was assessed in mouse plasma samples using the Human XL Cytokine Luminex Performance Panel Premixed Kit (#FCSTM18, R&D Systems), according to the manufacturer’s instructions.
-
Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... The levels of adipokines in the plasma of WT and BaKO mice were measured using the Mouse Adipokine Array Kit (ARY013; R&D Systems) and LEGENDplex (740929 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Naïve T cells were isolated by negative selection using a Mouse Naive T Cell CD4+/CD62L+/CD44low Column Kit (R&D Systems). Naïve CD4 T cells were treated with fluorescein isothiocyanate-labeled (FITC ...
-
bioRxiv - Cell Biology 2023Quote: ... An equivalent amount of CM (1.0 ml for each condition) was incubated with each nitrocellulose membrane provided in the Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems). Membranes were developed following the instructions of the manufacturer.
-
bioRxiv - Bioengineering 2024Quote: ... we used both mouse (111 analytes) and human (105 analytes) proteome profiler XL kits (R&D Systems, Cat No. ARY028 and ARY022B). We screened the cell culture supernatant from Day 7 spheroid cultures with and without SIS ECM ...
-
bioRxiv - Developmental Biology 2020Quote: ... Mouse EPO (R&D systems) was added to a final concentration of 10 units/ml ...
-
bioRxiv - Cancer Biology 2020Quote: ... Presence of 105 proteins in supernatants was evaluated using human cytokine and chemokine XL proteome array kit from R&D Systems (Minneapolis, MN).
-
bioRxiv - Biochemistry 2023Quote: ... and 250 μg of protein from each condition was incubated with a membrane array from the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare) ...
-
bioRxiv - Bioengineering 2021Quote: ... Serum IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Cancer Biology 2020Quote: Concentrations of murine IL-11 in the culture supernatants were determined by ELISA according to the manufacturers’ instruction (R&D Systems).
-
bioRxiv - Neuroscience 2021Quote: Human soluble E-selectin levels were assayed using quantitative sandwich enzyme immunoassay technique and following the manufacturer’s protocol (Quantikine ELISA Human E-selectin/CD62E Immunoassay, DSLE00, R&D Systems). Briefly ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were later determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Bioengineering 2021Quote: ... Total TGF-β1 levels were assessed using Human TGF-beta 1 DuoSet® ELISA (DY240; R&D Systems™, Minneapolis, MN). Similarly ...
-
bioRxiv - Bioengineering 2020Quote: Expression levels of cell secreted Osteoprotegerin and Vascular Endothelial Growth Factor (VEGF) was examined via ELISA (R&D Systems, Minnesota, USA). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
-
bioRxiv - Neuroscience 2022Quote: ... Interleukin-6 was measured in duplicate using a commercially available high-sensitive enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis) with a minimum detection threshold of 0.2 pg/ml ...
-
bioRxiv - Immunology 2021Quote: ... In-Cell ELISA was then developed using DY999 substrate solution according to the manufacturer’s recommendations (R&D Systems, Minneapolis, MN USA) and measured at 450 nm (and at 630 nm to assess the background ...
-
bioRxiv - Systems Biology 2022Quote: ... Levels of lymphocyte-derived cytokines (IFNγ, IL-17) were determined after 7 days (PeliKine Compact or Duoset ELISA, R&D Systems). Baseline inflammatory plasma markers from both cohorts ...
-
bioRxiv - Bioengineering 2020Quote: ... and the unbound growth factors in the supernatant were measured using enzyme-linked immunosorbent assay (ELISA; R&D Systems, Minneapolis, MN). For the other loading procedure (G3) ...
-
bioRxiv - Microbiology 2020Quote: ... Murine KC and JE concentrations were determined in the lung homogenate supernatants by DuoSet® ELISA Development Systems (R&D Systems), according to manufacturer instructions.
-
bioRxiv - Cell Biology 2019Quote: ... Luminex® Multiplex and ELISA assays for the detection of chemokines and cytokines were purchased from R&D Systems (Minneapolis, MN).
-
bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
-
bioRxiv - Genetics 2022Quote: Supernatants from transfected HEK293T cells were analyzed for their LBP content by Human LBP DuoSet ELISA (#DY870-05, R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... Plasma and urine GDF15 were measured by ELISA test (MG150 and DGD150, for rodent and human GDF15, respectively, R&D Systems) and tissue GDF15 was measured using an ELISA test (Elabscience).
-
bioRxiv - Microbiology 2023Quote: ... Supernatants are collected and frozen at -20°C for cytokine assays performed by ELISA according to manufacturer’s instructions (DuoSet, R&D Systems).
-
bioRxiv - Cancer Biology 2023Quote: ... Harvested CM was normalized to the fibroblast cell count and assessed for IGFBP2 using ELISA purchased from R&D system (DGB200). Serum samples were diluted 50-fold ...
-
bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
-
bioRxiv - Immunology 2023Quote: ... Just prior to analysis plasma samples were thawed and then assayed for numerous analytes (using dilutions optimized in prior studies) according to the manufacturer’s guidelines: adiponectin using the adiponectin/Acrp30 ELISA (DY1065, R&D System, lower limit of detection [LLoD] 15.6pg/ml) ...