Labshake search
Citations for R&D Systems :
1601 - 1650 of 1690 citations for Recombinant Rabbit KITLG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
-
bioRxiv - Cell Biology 2019Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
-
bioRxiv - Neuroscience 2019Quote: ... Plasma interleukin (IL)-6 and lipopolysaccharide-binding protein (LBP) levels were measured by commercially available enzyme-linked immunosorbent assay (R&D Systems, Minneapolis ...
-
bioRxiv - Bioengineering 2019Quote: ... To induce differentiation FGF2 was withdrawn from the cell culture media and then replaced with bone morphogenic protein (BMP4; R&D Systems) or and Wnt3a (R&D Systems) ...
-
bioRxiv - Immunology 2020Quote: ... cells were preincubated for 5 min at room temperature with 1 µg of the unlabeled PS-binding proteins MFG-E8 (R&D systems) and AnV (Biolegend) ...
-
bioRxiv - Microbiology 2022Quote: ... IL-6 protein was detected by Hamster IL-6 Sandwich ELISA Kit (AssayGenie) or Mouse IL-6 Duoset Sandwich ELISA kit (R&D Systems) according to manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were quantified by BCA assay and further equal protein was assayed by enzyme-linked immunosorbent assay (ELISA) for TNF-a using the manufacturer’s protocol (R&D systems, USA).
-
bioRxiv - Neuroscience 2024Quote: ... Relative expression levels of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2023Quote: ... after which the organoids were cultured for 13 days in E6 supplemented with 50 ng/mL bone morphogenetic protein-7 (BMP7, R&D systems) and 10 ng/mL human epidermal growth factor (hEGF ...
-
bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
-
bioRxiv - Immunology 2023Quote: ... Levels of IL1β protein were determined in culture supernatant using the IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
-
bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
-
bioRxiv - Cancer Biology 2023Quote: We assessed binding to a library of 300 glycan structures for Fc fusion proteins sourced from a commercial vendor (R&D Systems) composed of a fusion of Siglec-3 ...
-
bioRxiv - Neuroscience 2023Quote: ... Relative expression level of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
-
bioRxiv - Immunology 2023Quote: ... Unpurified transient transfection supernatant samples (50 µl) were pre-incubated with biotinylated SARS-CoV-2 spike protein (R&D Systems cat# BT10549) (50 µl ...
-
bioRxiv - Molecular Biology 2023Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...
-
bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
-
bioRxiv - Immunology 2023Quote: ... KIR2DL3*001 (#2014-KR-050) and KIR2DS4*001 (#1847-KR-050) KIR-Ig (KIR-Fc) fusion proteins were purchased from R&D systems. KIR2DS1*001-Fc was custom produced by Sino Biological ...
-
bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
-
bioRxiv - Immunology 2023Quote: ... Levels of BAFF protein were determined in culture supernatant or bone marrow plasma using the human BAFF/BLyS/TNFSF13B DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
-
bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
-
bioRxiv - Cancer Biology 2023Quote: ... The amount of CXCL1 or CXCL2 protein in the supernatant was determined using a mouse CXCL1 or CXCL2 specific ELISA kit (R&D system). All experiments were performed according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... co-cultures were maintained for 24 hours in the control condition or in the presence of the chimera proteins Fc-LGR4 (8077-GP, R&D Systems), Fc-LGR5 (8078-GP ...
-
bioRxiv - Immunology 2024Quote: ... For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050, R&D Systems) at 1 µg/ml.
-
bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
-
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... and acute phase proteins (total 36) present in the supernatants were detected with using Proteome Profiler Human Cytokines Array Kit (R&D Systems) according to manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... sections were incubated with horseradish peroxidase (HRP)-conjugated donkey anti-goat or goat anti-rabbit secondary antibody (R&D system and Santa Cruz), before incubation with diaminobenzidine substrate as a chromogen (Dako ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then subjected to western blot analysis detailed above to detect autophosphorylation of TAOK2 at S181 using the rabbit antibody (R&D Systems, PPS037).
-
bioRxiv - Neuroscience 2021Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
-
bioRxiv - Genetics 2019Quote: ... sections were incubated with horseradish peroxidase (HRP)-conjugated donkey anti-goat or goat anti-rabbit secondary antibody (R&D system and Santa Cruz), before incubation with diaminobenzidine substrate as a chromogen (Dako ...
-
bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then subjected to western blot analysis detailed above to detect TAOK2 autophosphorylation by rabbit antibody against pS181 (R&D Systems, PPS037).
-
bioRxiv - Neuroscience 2022Quote: ... 1:500, rabbit anti-PDGFRα (3164 Cell Signalling Technologies, Danvers, Massachusetts) 1:500 and goat anti-Sox10 (AF2864 R&D Systems, Minneapolis, Minnesota) 1:50 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A multiplex primary antibody mix was prepared in milk-free antibody diluent using Rabbit anti-DYKDDDK antibody (R&D Systems MAB8529; 1:1200) and mouse anti-Beta actin (R&D Systems MAB8929 ...
-
bioRxiv - Cell Biology 2023Quote: ... the membrane was probed with an anti-EFA6R rabbit polyclonal antibody (1:500) (8) or an anti-β-actin mouse monoclonal antibody (1:10,000) (R&D Systems, UK) diluted in blocking buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... slides for ACE-2 single stain and slides for ACE-2 stain were incubated with the Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100 ...
-
bioRxiv - Immunology 2024Quote: ... followed by incubation with primary antibodies (rabbit-anti RFP, Rockland, diluted 1:1500 and goat anti-PDGFRα, R&D Systems, diluted 1:500) in 1% BSA ...
-
bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
-
bioRxiv - Cancer Biology 2021Quote: ... 50μl of mitochondria (1μg/μl protein) were treated with Caspase-8-cleaved tBid from 5-500nM (882-B8-050, R&D Systems, Minneapolis, MN), Bim-BH3 from 10-10,000nM (Ac-DMRPEIWIAQELRRIGDEFNAYYARR-amide ...
-
bioRxiv - Cancer Biology 2020Quote: ... Presence of 105 proteins in supernatants was evaluated using human cytokine and chemokine XL proteome array kit from R&D Systems (Minneapolis, MN).
-
bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
-
bioRxiv - Cancer Biology 2022Quote: ... with good affinity and specificity were screened and selected from the homemade human single-chain antibody library through commercial VEGF and EGFR proteins (R&D Systems, Inc.). Then ...
-
bioRxiv - Neuroscience 2022Quote: ... A subset of slices were infected with only AAV9.hsyn.GFP and at DIV6 were treated with human Sema4D-Fc ectodomain/human Fc fusion protein (Sema4D-Fc; R&D Systems, #7470-S4) or Fc control protein (R&D Systems ...
-
bioRxiv - Genomics 2022Quote: Circulating AAV-expressed human ANGPTL3 protein levels were measured from plasma using commercially-available ELISA kits (plasma diluted 1:4 and used with R&D Systems #DANL30). The assay is specific for detection of human ANGPTL3 protein ...
-
bioRxiv - Microbiology 2022Quote: ... The HA remaining after supernatant-driven degradation was detected with HA binding protein following dilutions and instructions in the Hyaluronan DuoSet ELISA kit by R&D Systems (DY3614). As was the case for fibronectin and laminin ...
-
Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ICAM-1 and VCAM-1 protein levels were evaluated by SDS PAGE using goat anti-Icam-1 (R&D systems Cat# AF-796) and rabbit anti-Vcam-1 (abcam ...
-
bioRxiv - Immunology 2024Quote: ... Protein lysates were subjected to enzyme-linked immunosorbent assay (ELISA) using mouse DuoSet® ELISA commercial kits (R&D Systems, Minneapolis, MN, USA), following manufacturer instructions.
-
bioRxiv - Biochemistry 2023Quote: ... and 250 μg of protein from each condition was incubated with a membrane array from the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare) ...