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Citations for R&D Systems :
1551 - 1600 of 2461 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: HCF-conditioned media were applied to the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). 5ml of conditioned medium from H2O2 or H2O treated HCFs was concentrated into 500μl using MilliporeSigma Protein-Concentrate Kit (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: PBMC were isolated from whole blood and treated with recombinant human CCL2 (279-MC-010; R&D Systems) at 100 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... The lysed LVV solution was assessed using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) as described previously76 ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used for FACS: Human/mouse/bovine integrin alpha 6/CD49f PE-conjugated antibody (FAB13501P, R&D Systems); PE/Cyanine 7 anti-mouse CD325 (Ep-CAM ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 μl per well of 1.5 μg/ml human recombinant integrin αvβ6 (R&D Systems) diluted in coating buffer from the ELISA Starter Accessory Kit (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2023Quote: Recombinant Human Neuronal Pentraxin 1 Protein (NP1) was purchased from R&D systems (#: 7707-NP, bio-techne, USA).
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bioRxiv - Microbiology 2023Quote: ... Cells were pretreated with vehicle or 50 ng/mL recombinant human IL-8 (rhIL-8, R&D Systems) 12 h before scratch assay ...
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bioRxiv - Molecular Biology 2023Quote: We used the proteome profiler human apoptosis array kit from R&D Systems (Mineapolis, MB, USA, Cat. ARY009) to assess apoptosis and apoptosis-related proteins according to manufactures instructions ...
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bioRxiv - Bioengineering 2023Quote: ... medium was changed every day to HDM with 20 ng/mL human recombinant BMP-4 (R&D Systems) and 20 ng/mL human recombinant FGF-4 (R&D Systems) ...
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bioRxiv - Cell Biology 2024Quote: ... COL1A1 was measured using the Human pro-collagen 1A1 ELISA kit (R&D Systems, cat. no. DY6220-05) in a 1:50 efflux dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... and CAL27 were screened via flow cytometry using an APC-conjugated human ITGB6 antibody (R&D Systems, # FAB4155A). The mouse cell lines CT26 ...
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bioRxiv - Cancer Biology 2024Quote: ... ENAS + 5 ng/ml TGF-β1 (Recombinant Human TGF-β1 Protein, R&D Systems, Cat. No. 240-B). Basal medium + solvent ...
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bioRxiv - Cancer Biology 2024Quote: ... The Human IL-1 beta/IL-1F2 DuoSet ELISA 96-wells (R&D Systems, Cat. No. DY201-05) and the Wnt-5a ELISA kit (Human) ...
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bioRxiv - Bioengineering 2024Quote: ... COL1A1 was measured using the Human pro-collagen 1A1 ELISA kit (R&D Systems, cat. no. DY6220-05) in a 1:50 efflux dilution ...
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bioRxiv - Bioengineering 2024Quote: ... This was done by incubating 100 µg of recombinant human G-CSFR (381-GR/CF, R&D Systems) in 950 µL PBS for 2 h at room temperature (RT ...
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bioRxiv - Genetics 2024Quote: ... incubated with goat anti-PLZF primary antibody at 1:500 dilution (Human PLZF Antibody, R&D Systems AF2944) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... Forty-five analytes were measured using diluted plasma and Human XL Cytokine Discovery Premixed Kit (R&D System). Samples were thawed on ice and diluted 1:2 with calibration diluent ...
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bioRxiv - Immunology 2024Quote: ... coated with 1 µg/ml (volume 100 μl/well) of human recombinant IFN specific proteins (R&D Systems) in Phosphate Buffered Saline (PBS) ...
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bioRxiv - Immunology 2023Quote: ... and were cultured with 50U/ml hIL-2 (Th0) or 100U/ml hIL-2 and 5ng/ml murine TGFβ (R&D Systems, 7666-MB/CF) for 4-5 days.
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial cells were then incubated with 0.25 μg of purified human SP-D (R&D Systems, 1920-SP-050), ZAG (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... Primary neurons and EVs were pre-incubated with active recombinant human CD59 (cat. no. 1987-CD, R&D Systems) at a final concentration of 1 μM (stock at 10 μM ...
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bioRxiv - Immunology 2019Quote: ... and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems, Ref. HS600C Lot P178994). All these immunoassays were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... TNF-α was measured using a Quantikine HS ELISA Human TNF-α (R&D Systems, Ref. HSTA00E Lot P174731) and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... CXCL8 and Osteopontin (SPP1) proteins in conditioned media was performed using a custom human luminex assay (R&D systems), according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: Phosphorylation status and total amounts of 43 kinases were measured using Human Phospho-Kinase Array from R&D Systems. 1.5×106 LN229 cells were stimulated with 400 ng/mL of GAS6 for 10 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... with 10% FBS (Wisent) and 0.5 ng/ml recombinant human Macrophage Colony Stimulating factor (M-CSF) (R&D Systems) to allow for differentiation of monocytes into macrophages ...
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bioRxiv - Bioengineering 2020Quote: ... 97 µL of urine was incubated with 1 µL of human cathepsin B (R&D Systems, 953-CY-010) and 1 µL of DTT for 15 minutes to inactivate the urine cysteine protease inhibitor ...
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bioRxiv - Physiology 2021Quote: ... The medium was supplemented with 100 ng/mL recombinant human M-CSF using CellXVivo™ kit (R&D Systems) and differentiated for 5–7 days prior to use.
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against human DDR1 (R&D Systems, AF2396), CXCL5 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with 10 ng ml−1 of recombinant human IFNγ (R&D Systems, 285-IF-100), 10 ng ml−1 of recombinant human TNFα (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... For the IL-8 neutralization experiments: 0.4 μg/ml of human IL-8 monoclonal antibody (R&D Systems, USA) was added to the apical and basolateral compartment of enteroid monolayers and to the PMN suspensions before they were co-cultured ...
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bioRxiv - Immunology 2022Quote: Human IL-8 was measured from cell culture supernatants using commercially available specific paired ELISA kits (R&D Systems). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... IFN-β amounts in the culture supernatant were measured by human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Immunology 2020Quote: ... and Alexa Fluor 594 labelled anti-human DAP10/HCST (R&D Systems, 1:500 v/v in blocking buffer) to stain for the cytoskeleton and DAP10 clusters respectively ...
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bioRxiv - Physiology 2021Quote: ... The membranes were incubated with a sheep polyclonal antibody to human DG (AF6868; R&D Systems, 1:100 dilution) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6 ...
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bioRxiv - Biochemistry 2021Quote: For TCR-mediated adhesion 96-well plates were pre-coated with 0.5μg human ICAM-1/well (R&D systems). Transfected cells were left untreated or stimulated with anti-TCR antibody (OKT3 ...
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bioRxiv - Physiology 2022Quote: ... Aβ ELISA was performed using a human amyloid β (aa1-42) immunoassay Quantikine ELISA kit (R&D Systems, Inc.).
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bioRxiv - Molecular Biology 2022Quote: ... human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF, 50 ng/mL R&D Systems, 7954-GM-020/CF), and interleukin-4 (IL-4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of GDF15 was measured using an ELISA assay for Human GDF-15 DuoSet (R&D systems, # DY957) as per the manufacturer’s instructions on a Tecan INFINITE® 200 Pro spectrophotometer ...
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bioRxiv - Pathology 2020Quote: ... the membranes were incubated with the sheep anti-human collagen I alpha 1 antibody (1:2000, R&D systems) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was titered using a commercial enzyme-linked immunosorbent assay kit (Human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml−1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells surface ACE2 protein was stained with Alex Flour® 647-conjugated human ACE2 antibody (R&D Systems FAB933R). Live/Dead (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The level of human or mouse CXCL10/IP-10 was determined using a DuoSet ELISA kit (R&D Systems) and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were exposed to the Human/Mouse Brachyury Affinity Purified Polyclonal Goat IgG antibody (R&D Systems, Catalog # AF2085) overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell-free supernatant was collected and quantified using the Human IL-12p70 DuoSet ELISA (R&D Systems; catalog# DY1270), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...
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bioRxiv - Immunology 2020Quote: ... and 10 ng/mL of human macrophage colony stimulating factor (M-CSF, R&D Systems, catalog number 216-MC) for at least 10 days to generate mature macrophages before use in experiments.