Labshake search

1501 - 1550 of 2794 citations for Recombinant Mouse IL21 Protein since 2019

• Aged Breast Matrix Bound Vesicles Promote Breast Cancer Invasiveness

  Jun Yang, et al., bioRxiv - Bioengineering 2023

  Quote: Cytokine profiling of the MBVs isolated from the aged and young mouse breast ECMs was performed using the dot blot-based Proteome Profiler Mouse XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions ...
• The staphylococcal type VII secretion system impacts daptomycin sensitivity through controlling bacterial cell envelope integrity

  Victoria Smith, et al., bioRxiv - Microbiology 2023

  Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
• Inhibition of EGFR/ErbB does not protect against C. difficile toxin B

  Uswah Siddiqi, et al., bioRxiv - Microbiology 2024

  Quote: ... Lysates of 3 mouse colons from each group were pooled together and used as a substrate for the mouse Proteome Profiler Array (R&D Systems) following the manufacturer’s protocol.
• Massively parallel screening of TIR-derived peptides reveals vast TLR-targeting immunomodulatory peptides

  Yun Lim, et al., bioRxiv - Molecular Biology 2024

  Quote: ... The supernatants were transferred to a new eppendorf tube and used for measuring the levels of cytokines using the Mouse TNF-alpha Quantikine ELISA kit and the Mouse IL-6 Quantikine ELISA kit (R&D systems), following the manufacturer’s instructions.
• Synthetic amyloid beta does not induce a robust transcriptional response in innate immune cell culture systems

  I.Y. Quiroga, et al., bioRxiv - Neuroscience 2021

  Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
• Chronic IL-1 exposure drives LNCaP cells to evolve androgen and AR independence

  H.C. Dahl, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
• Structural insights into CXCR4 modulation and oligomerization

  Kei Saotome, et al., bioRxiv - Biochemistry 2024

  Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
• Interleukin-6 induces nascent protein synthesis in human DRG nociceptors via MNK-eIF4E signaling

  Molly E. Mitchell, et al., bioRxiv - Neuroscience 2024

  Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
• Protein SUMOylation promotes cAMP-independent EPAC1 activation

  Wenli Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
• Virological characteristics of the SARS-CoV-2 Omicron EG.5.1 variant

  Shuhei Tsujino, et al., bioRxiv - Microbiology 2023

  Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
• Development and IND-enabling studies of a novel Cas9 genome-edited autologous CD34⁺ cell therapy to induce fetal hemoglobin for sickle cell disease

  Varun Katta, et al., bioRxiv - Genomics 2024

  Quote: ... 3 units/mL EPO (Amgen, EPOGEN NDC 55513-144-01), 200 μg/mL holo-transferrin (Millipore Sigma, T0665, 10 ng/mL recombinant human SCF (R&D systems, 255-SC/CF), and 1 ng/mL recombinant human interleukin IL-3 (R&D systems ...
• Transcription of HIV-1 at sites of intact latent provirus integration

  Ana Rafaela Teixeira, et al., bioRxiv - Immunology 2024

  Quote: ... cells were maintained in resting conditions by culturing in simple R10 or R10 supplemented with 10 ng/mL of recombinant human interleukin-7 (IL-7, R&D Systems, cat. BT-007-010) for one week.
• Defining Epidermal Stem Cell Fate Infidelity and Immunogenicity in Hidradenitis Suppurativa at the Single-Cell Resolution

  Meaghan Marohn, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-Sox10 (1:800, R&D Systems, MAB2864), rabbit anti-Sox9 (1:1000 ...
• A non-invasive method to generate induced pluripotent stem cells from primate urine

  Johanna Geuder, et al., bioRxiv - Cell Biology 2020

  Quote: ... Human/Mouse alpha-Fetoprotein/AFP (R&D Systems, MAB1368), Nanog (R&D Systems ...
• Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts

  Lara-Jane Kepser, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-GAPDH (1:10,000, #MAB5718, R&D Systems)
• Zfp36l1 and Zfp36l2 balances proliferation and differentiation in the developing retina

  Fuguo Wu, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-Nr2e3 (R&D systems PP-H7223,1:100), goat anti-Nrl (R&D systems AF2945 ...
• Crosstalk between Fat Mass and Obesity-related (FTO) and multiple WNT signaling pathways

  Hyunjoon Kim, Soohyun Jang, Young-suk Lee, bioRxiv - Molecular Biology 2021

  Quote: ... human/mouse Wnt5a (R&D Systems #645-WN-010) and human R-Spondin1 (R&D Systems #4645-RS-025 ...
• The antimicrobial peptide cathelicidin is critical for the development of Th17 responses in severe inflammatory disease

  Katie J Smith, et al., bioRxiv - Immunology 2022

  Quote: Concentrations of mouse IL-23 (R&D Systems, #DY1887) and IL-12 (R&D Systems #M1270 ...
• Gastric metabolomics analysis supports H. pylori’s catabolism of organic and amino acids in both the corpus and antrum

  Daniela Keilberg, et al., bioRxiv - Microbiology 2020

  Quote: ... 1 μg/mL mouse R-spondin (R&D systems), 100 ng/mL Noggin (Peprotech) ...
• FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetes

  Christopher Yanucil, et al., bioRxiv - Molecular Biology 2021

  Quote: ... mouse FGF21 (8409-FG-025/CF, R&D Systems), human soluble β-klotho (5889-KB-050/CF ...
• S1P-S1PR1 activity controls VEGF-A signaling during lymphatic vessel development

  AM Golding-Ochsenbein, et al., bioRxiv - Developmental Biology 2019

  Quote: ... goat anti-mouse VEGFR-2 (R&D Systems, AF644). The next day ...
• Strain-level differences in gut microbiome composition determine fecal IgA levels and are modifiable by gut microbiota manipulation

  Chao Yang, et al., bioRxiv - Immunology 2019

  Quote: ... goat anti-mouse pIgR (R&D Systems, cat #: AF2800). Slides were washed in PBS for three times ...
• Coexpression of CCR7 and CXCR4 during B cell development controls CXCR4 responsiveness and bone marrow homing

  Saria Mcheik, et al., bioRxiv - Immunology 2019

  Quote: ... allophycocyanin anti-mouse CXCR5 (clone 614641, R&D Systems) and allophycocyanin rat anti-mouse CCR6 (clone 140706 ...
• Nestin in immature embryonic neurons regulates axon growth cone morphology and Sema3a sensitivity

  Christopher J Bott, et al., bioRxiv - Cell Biology 2019

  Quote: ... cell were treated with mouse Sema3a (R&D systems) diluted in growth media for 5 minutes at 1nM ...
• Hydrogel-based slow release of a receptor-binding domain subunit vaccine elicits neutralizing antibody responses against SARS-CoV-2

  Emily C. Gale, et al., bioRxiv - Bioengineering 2021

  Quote: ... Mouse TNFa Quantikine ELISA kit (R&D Systems, SMTA00B), and Legend Max Mouse CXCL13 (BLC ...
• Aptamer based spatiotemporally controlled growth factor patterning for tunable local microvascular network formation in engineered tissues

  Deepti Rana, et al., bioRxiv - Bioengineering 2020

  Quote: ... human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H+L ...
• Salmonella exploits OmpR to switch essential morphogenetic peptidoglycan synthases in response to intracellular cues

  David López-Escarpa, et al., bioRxiv - Microbiology 2021

  Quote: ... mouse monoclonal anti-6xHis (1:2500; R&D Systems), rabbit polyclonal anti-PBP2 (1:1000 ...
• Nestin selectively facilitates the phosphorylation of the Lissencephaly-linked protein doublecortin (DCX) by cdk5/p35 to regulate growth cone morphology and Sema3a sensitivity in developing neurons

  Christopher J. Bott, et al., bioRxiv - Cell Biology 2020

  Quote: ... cells were treated with mouse Sema3a (R&D systems) diluted in growth media for 5 ...
• The interaction of enteric bacterial effectors with the host engulfment pathway control innate immune responses

  Ibrahim M Sayed, et al., bioRxiv - Microbiology 2021

  Quote: ... using a mouse ELISA kit (R&D Systems, USA) according to the manufacturer’s instructions.
• BK polyomavirus evades innate immune sensing by disrupting the mitochondrial network and membrane potential and by promoting mitophagy

  Julia Manzetti, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse monoclonal IgG2b anti-STING (MAB7169, R&D Systems), polyclonal rabbit anti-LC3A/B (4108S ...
• Reversal of the renal hyperglycemic memory by targeting sustained tubular p21 expression

  Moh’d Mohanad Al-Dabet, et al., bioRxiv - Molecular Biology 2021

  Quote: ... goat anti-mouse nephrin (AF3159; R&D systems, USA). The following secondary antibodies for immunofluorescence were used ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse IgG2a anti-Tubb3 Tuj1 (MAB1995 R&D systems), mouse IgG1 anti-SV2 (DSHB) ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-mouse IgG2a 488 (Alexa Fluor R&D systems), anti-rabbit 488 (Alexa Fluor R&D systems ...
• Mechanisms Underlying WNT-mediated Priming of Human Embryonic Stem Cells

  Anna Yoney, et al., bioRxiv - Developmental Biology 2022

  Quote: ... EOMES (mouse monoclonal, R&D Systems MAB6166, 1:400), GSC (goat polyclonal ...
• Next-generation intranasal Covid-19 vaccine: a polymersome-based protein subunit formulation that provides robust protection against multiple variants of concern and early reduction in viral load of the upper airway in the golden Syrian hamster model

  Jian Hang Lam, et al., bioRxiv - Immunology 2022

  Quote: ... mouse anti-S1 mAb (clone 1035206; R&D Systems) was diluted 1:100 and applied to each well ...
• Combined single-cell RNA-seq profiling and enhancer editing reveals critical spatiotemporal controls over thalamic nuclei formation in the murine embryo

  Kiya W. Govek, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti- Sox2 (1:100, R&D Systems, MAB2018), rabbit anti-Sox2 (1:300 ...
• Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses

  Marijke I. Zonneveld, et al., bioRxiv - Immunology 2020

  Quote: ... mouse anti-human CSTB (clone 225228, R&D Systems, dilution 1:5.00 ...
• Adult Neurogenesis in Peripheral Nervous System

  Yisheng Liu, Xiaosong Gu, bioRxiv - Neuroscience 2020

  Quote: ... plexin C1 mouse 1:500 (R&D Systems AF5375), SSEA-1 mouse 1:200 (Millipore [clone MC-480] MAB4301) ...
• Temporal analysis of enhancers during mouse brain development reveals dynamic regulatory function and identifies novel regulators of cerebellar development

  Miguel Ramirez, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-Pax3 (1:500, R&D systems, MAB2457), rabbit anti-Pax2 (1:200 ...
• MYC promotes immune-suppression in TNBC via inhibition of IFN signaling

  Dario Zimmerli, et al., bioRxiv - Cancer Biology 2021

  Quote: ... proteome profiler Mouse XL Cytokine array (R&D system) was performed on whole cell lysate from WB1P and WB1P organoids ...
• RPTPγ is a redox-regulated suppressor of promigratory EGFR signaling

  Maitreyi S. Joshi, et al., bioRxiv - Systems Biology 2022

  Quote: ... mouse anti-TCPTP (MAB1930, R&D Systems, Minneapolis, MN), mouse anti-p22phox (ab80896 ...
• The C-type Lectin Receptor Dectin-2 is a receptor for Aspergillus fumigatus galactomannan

  Jennifer L. Reedy, et al., bioRxiv - Immunology 2022

  Quote: ... mouse anti-Dectin-2 antibody (R&D Systems, MAB1525) was preincubated with the Dectin-2-Fc fusion protein at a concentration of 50 mg/ml for 30 minutes prior to adding the mixture to FLPs.
• Distinct cancer-associated fibroblast states drive clinical outcomes in high-grade serous ovarian cancer and are regulated by TCF21

  Ali Hussain, et al., bioRxiv - Cancer Biology 2019

  Quote: ... mouse-anti-human-FAP (R&D Systems, 1:50), and either mouse-anti-pan-Cytokeratin (Abcam ...
• Genetic Variant in 3’ Untranslated Region of the Mouse Pycard Gene Regulates Inflammasome Activity

  Brian Ritchey, et al., bioRxiv - Genetics 2021

  Quote: ... 3 ng/ml mouse IL-3 (R&D Systems) and 20% L-cell conditioned medium ...
• Extratubular polymerized uromodulin induces leukocyte recruitment and inflammation in vivo

  Roland Immler, et al., bioRxiv - Immunology 2020

  Quote: ... mouse anti human CD106 (clone 4B2, R&D Systems), secondary goat anti mouse-PE (Pharmingen),mouse anti human CD18 high affinity confirmation (clone 24 ...
• Islet macrophages drive islet vascular remodeling and compensatory hyperinsulinemia in the early stages of diabetes

  Manesh Chittezhath, et al., bioRxiv - Molecular Biology 2019

  Quote: ... goat anti-mouse CD31 (R&D Systems, MN, USA), rabbit anti-mouse Ki67 (abcam ...
• An infectivity-enhancing site on the SARS-CoV-2 spike protein is targeted by COVID-19 patient antibodies

  Yafei Liu, et al., bioRxiv - Microbiology 2020

  Quote: Mouse anti-human ACE2 mAb (R&D Systems, USA), rat anti-Flag mAb (L5 ...
• Production and secretion of functional full-length SARS-CoV-2 spike protein in Chlamydomonas reinhardtii

  Anna Kiefer, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... mouse anti-RBD (MAB105401, R&D Systems, 1:2,000) and mouse anti-ACE2 (sc-390851 ...
• Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities

  Esther Redder, et al., bioRxiv - Cell Biology 2021

  Quote: ... goat polyclonal anti-mouse Lyve1 (R&D Systems, AF2125), rat monoclonal anti-mouse Lyve1 (clone 223322 ...
• IL-17A both initiates (via IFNγ suppression) and limits the pulmonary type-2 immune response to nematode infection

  Jesuthas Ajendra, et al., bioRxiv - Immunology 2020

  Quote: ... Mouse IL-13 DuoSet ELISA kit (R&D Systems) was used for measurement of IL-13 levels ...