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1501 - 1550 of 2503 citations for Recombinant Human ARSA His tagged since 2019

• Tuberculosis susceptibility in genetically diverse mice reveals functional diversity of neutrophils

  Mariëtta M. Ravesloot-Chávez, et al., bioRxiv - Immunology 2023

  Quote: ... BMDM were then treated with vehicle or recombinant mouse IFN-γ at 6.25 ng/ml (485-MI, R&D Systems) overnight and infected with Mtb Erdman strain at a multiplicity of infection of 5 in DMEM supplemented with 5% horse serum and 5% FBS ...
• HMGB1 acts as an agent of host defense at the gut mucosal barrier

  A-M C Overstreet, et al., bioRxiv - Microbiology 2023

  Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
• Cytosolic S100A8/A9 promotes Ca²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

  Matteo Napoli, et al., bioRxiv - Physiology 2024

  Quote: ... 24 well-plates were coated with recombinant murine (rm) E-selectin (rmCD62E-Fc chimera, 10µg mL-1, R&D Systems) or PBS/0.1% BSA at 4°C overnight ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... T cells were isolated and stained with anti- human CD3 and CD8a antibodies (surface staining) followed by intracellular staining with anti- human IFNγ (R&D Systems, Cat. No. IC285F-100) antibody ...
• Adenovirus-α-defensin complexes induce NLRP3-associated maturation of human phagocytes via TLR4 engagement

  Karsten Eichholz, et al., bioRxiv - Immunology 2021

  Quote: ... and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems). Twenty-two cytokines were detected using Bio-plex human chemokine ...
• Modeling Genetic Epileptic Encephalopathies using Brain Organoids

  Daniel J. Steinberg, et al., bioRxiv - Neuroscience 2020

  Quote: ... 100ng/ml human FLT-3 ligand (R&D Systems; 308-FKE), 20ng/ml human IL-3 (Peprotech ...
• Glycine targets NINJ1-mediated plasma membrane rupture to provide cytoprotection

  Jazlyn P. Borges, et al., bioRxiv - Cell Biology 2022

  Quote: ... Cells were incubated with human NINJ1 antibody (R&D Systems, MAB5105) at 10 μg/ml in PBS/0,01% saponin/1% pooled human serum at 4 °C for 41 hours ...
• Microglial Neuropilin-1 trans-regulates oligodendrocyte expansion during development and remyelination

  Amin Sherafat, et al., bioRxiv - Neuroscience 2021

  Quote: ... or the control human Fc dimer (R&D Systems, 100-HG) was added to slice cultures at the indicated concentrations for the last 48 hours of incubation ...
• Clr4^SUV39H1 and Bdf2^BRD4 ubiquitination mediate transcriptional silencing via heterochromatic phase transitions

  Hyun-Soo Kim, et al., bioRxiv - Molecular Biology 2021

  Quote: ... 0.9 μg/μl of human ubiquitin (R&D systems U-100H) or buffer only (10 mM Hepes ...
• An Endogenously activated antiviral state restricts SARS-CoV-2 infection in differentiated primary airway epithelial cells

  Lindsay Broadbent, et al., bioRxiv - Immunology 2021

  Quote: ... 1 or 100 ng/mL human IFNλ3 (R&D systems, Biotechne) for 24 h ...
• FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetes

  Christopher Yanucil, et al., bioRxiv - Molecular Biology 2021

  Quote: ... human soluble β-klotho (5889-KB-050/CF, R&D Systems), human FGFR1c (658FR050) ...
• Chromatin and transcriptional response to loss of TBX1 in early differentiation of mouse cells

  Andrea Cirino, et al., bioRxiv - Genomics 2020

  Quote: ... 10 ng/ml human bFGF (R&D Systems 233-FB-025), and 50 ng/ml human FGF10 (R&D Systems 338-FG-025) ...
• Chromatin and transcriptional response to loss of TBX1 in early differentiation of mouse cells

  Andrea Cirino, et al., bioRxiv - Genomics 2020

  Quote: ... and 5ng/ml human VEGF (R&D Systems Cat#. 293-VE). The 2-day-old EBs were dissociated and 6×104 cells were seeded onto individual wells of a 24-well plate coated with 0.1% gelatin in StemPro-34 medium (Gibco #10639011) ...
• High glucose enhances antigen-independent CTL killing via TRAIL

  Wenjuan Yang, et al., bioRxiv - Immunology 2021

  Quote: ... human TRAIL R1/TNFRSF 10A PerCP-conjugated antibody (R&D Systems), and human TRAIL R4/TNFRSF 10D PE- conjugated antibody (R&D Systems) ...
• PlexinA4-Semaphorin3A mediated crosstalk between main cortical interneuron classes is required for superficial interneurons lamination

  Greta Limoni, et al., bioRxiv - Neuroscience 2020

  Quote: ... or 20µg goat anti-human neuropilin2 Fc chimera (R&D System) peptides before application of 5nM of SEMA3A as described before ...
• Spontaneous regression of micro-metastases following primary tumor excision: a critical role for primary tumor secretome

  Lee Shaashua, et al., bioRxiv - Cancer Biology 2020

  Quote: Human Cytokine Array (R&D systems, Proteome Profiler™ Array, ARY022) was used to compare relative expression levels of 102 soluble human proteins in CM samples ...
• PTEN inhibits AMPK to control collective migration

  Florent Peglion, et al., bioRxiv - Cell Biology 2021

  Quote: The human Phospho-kinase array kit (R&D Systems, Ref#ARY003B) was used to perform the small phosphoprotemics screen to unveil new targets affected by PTEN depletion ...
• Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells

  Nuria Vilaplana-Lopera, et al., bioRxiv - Cell Biology 2021

  Quote: ... 50 ng/ml human SCF (255-SC-010, R&D Systems), 10 ng/ml human IL-3 (203-IL-010 ...
• Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis

  Alex Rosenberg, L. David Sibley, bioRxiv - Microbiology 2020

  Quote: ... Bradyzoites were either stimulated with human IFN-γ (R&D systems) at final experimental concentration of 100 U/ml or IFN-β at final experimental concentration of 1000 U/ml for 15 hr after 5 days in differentiation media or left unstimulated ...
• CLEC5A and TLR2 are critical in SARS-CoV-2-induced NET formation and lung inflammation

  Pei-Shan Sung, et al., bioRxiv - Immunology 2022

  Quote: ... goat anti-human/mouse myeloperoxidase polyclonal antibody (# AF3667, R&D system). Antibodies for immunohistochemical (IHC ...
• CLEC5A and TLR2 are critical in SARS-CoV-2-induced NET formation and lung inflammation

  Pei-Shan Sung, et al., bioRxiv - Immunology 2022

  Quote: ... goat anti-human/mouse myeloperoxidase polyclonal antibody (#AF3667, R&D system), anti-CD11b antibody (#ab13357 ...
• The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not antigen-experienced vaccinees

  Julie Van Coillie, et al., bioRxiv - Immunology 2022

  Quote: ... or anti-human FUT8 (R&D Systems, polyclonal sheep IgG; #AF5768), or isotype sheep control IgG (R&D Systems) ...
• The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not antigen-experienced vaccinees

  Julie Van Coillie, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human ST6GAL1 (R&D Systems, polyclonal goat IgG Ab; #AF5924), or isotype goat control IgG (R&D Systems) ...
• SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D

  Madeline J. Lee, et al., bioRxiv - Immunology 2022

  Quote: ... and Human ULBP-2 DuoSet ELISA (R&D Systems, Cat. DY1298) kits according to the manufacturer’s instructions ...
• 3D Bioprinted perfusable and vascularized breast tumor model for dynamic screening of chemotherapeutics and CAR-T cells

  Madhuri Dey, et al., bioRxiv - Bioengineering 2022

  Quote: ... Proteome Profiler Human XL Oncology Array (R&D Systems, Minneapolis, MN) was used to identify 84 different cancer-related antibodies ...
• A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic target

  Ana Vujovic, et al., bioRxiv - Cancer Biology 2022

  Quote: ... human interleukin-3 (IL-3, 10 ng/mL, R&D Systems), human interleukin-6 (IL-6 ...
• A tumour-promoting senescent secretome triggered by platinum chemotherapy exploits a targetable TGFβR1/Akt-mTOR axis in lung cancer

  Estela González-Gualda, et al., bioRxiv - Cancer Biology 2022

  Quote: ... the Human TGF-beta 2 Quantikine ELISA Kit (R&D Systems), and the Human TGF-beta 3 DuoSet ELISA Kit (R&D Systems).
• Translational control of breast cancer plasticity

  Michael Jewer, et al., bioRxiv - Cancer Biology 2019

  Quote: – Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody (R&D Systems, Minneapolis ...
• Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion

  Emilly S. Villodre, et al., bioRxiv - Cancer Biology 2021

  Quote: Human Lipocalin-2/NGAL Quantikine ELISA Kits (#DLCN20, R&D Systems) were used to measure the levels of LCN2 in the cell lines according to the manufacturer’s instructions ...
• Extratubular polymerized uromodulin induces leukocyte recruitment and inflammation in vivo

  Roland Immler, et al., bioRxiv - Immunology 2020

  Quote: ... mouse anti human IgG1 isotype control (clone 11711, R&D Systems), mouse anti human CD106 (clone 4B2 ...
• CAR-NK Cells Effectively Target the D614 and G614 SARS-CoV-2-infected Cells

  Minh Ma, et al., bioRxiv - Immunology 2021

  Quote: ... PE anti-human NKG2C/CD159c antibody (clone 134591, R&D Systems) were purchased from R&D Systems ...
• Developing a xenograft model of human vasculature in the mouse ear pinna

  Gavin R. Meehan, et al., bioRxiv - Pathology 2019

  Quote: ... 2µl drops of human ICAM-1 (R&D Systems, MN, USA), mouse ICAM-1 (R&D Systems ...
• Secretory cells are the primary source of pIgR in small airways

  Jessica B. Blackburn, et al., bioRxiv - Cell Biology 2021

  Quote: ... Scgb1a1 (rat anti-human, R&D systems, MAB4218, 1:50 amplified), Scgb1a1 (rabbit anti-mouse ...
• ETV2 primes hematoendothelial gene enhancers prior to hematoendothelial fate commitment

  Jeffrey D. Steimle, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 8 ng/ml human Activin A (R&D systems Cat. 338AC), 5 ng/ml mouse VEGF (R&D systems Cat ...
• An autocrine Vitamin D-driven Th1 shutdown program can be exploited for COVID-19

  Reuben McGregor, et al., bioRxiv - Immunology 2020

  Quote: Array (Proteome Profiler Human Phospho-Kinase Array Kit, R&D systems) was carried out as per manufacturer’s protocol ...
• The N-terminal domain of spike glycoprotein mediates SARS-CoV-2 infection by associating with L-SIGN and DC-SIGN

  Wai Tuck Soh, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse anti-human ACE2 mAb (R&D Systems, Minneapolis, MN, USA), rat anti-Flag mAb (Sigma-Aldrich ...
• Efficacy of Targeting SARS-CoV-2 by CAR-NK Cells

  Minh Ma, Saiaditya Badeti, Ke Geng, Dongfang Liu, bioRxiv - Immunology 2020

  Quote: ... PE anti-human NKG2C/CD159c antibody (clone 134591, R&D Systems) were purchased from R&D Systems ...
• Lactoferrin retargets adenoviruses to TLR4 to induce an abortive NLRP3-associated pyroptotic response in human dendritic cells

  Coraline Chéneau, et al., bioRxiv - Immunology 2020

  Quote: ... and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) following the manufacturer’s instructions ...
• A VLP-based vaccine targeting ANGPTL3 lowers plasma triglycerides in mice

  Alexandra Fowler, et al., bioRxiv - Immunology 2021

  Quote: ... or the N-terminal domain of human ANGPTL4 (R&D Systems) as the target antigens ...
• Antibody-induced glomerulonephritis is amplified by RTEC-intrinsic IL-17 signaling and restrained by IL-17-mediated induction of the endoribonuclease Regnase-1 (Zc3h12a)

  De-Dong Li, et al., bioRxiv - Immunology 2021

  Quote: ... Immunoblotting Abs: human/mouse anti-Regnase-1 (MAB7875, R&D Systems), human anti-β actin (ab49900 ...
• Release of Notch activity coordinated by IL-1β signalling confers differentiation plasticity of airway progenitors via Fosl2 during alveolar regeneration

  Jinwook Choi, et al., bioRxiv - Cell Biology 2021

  Quote: ... Rat anti-human SCGB1A1/CC10 (1:200, R&D system, MAB4218), Rabbit anti-KDR/VEGFR2 (1:100 ...
• SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks

  Brett Stern, Peter Monteleone, Janet Zoldan, bioRxiv - Bioengineering 2022

  Quote: ... we used a Proteome Profiler Human Cytokine Array (R&D Systems) using the recommended protocol for cell supernatant ...
• Targeted LNPs deliver mRNA encoding IL-15 superagonists to balance efficacy and toxicity in cancer therapy

  Juntao Yu, et al., bioRxiv - Bioengineering 2024

  Quote: ... Human IL-6 Quantikine ELISA Kit (R&D systems, MN, USA), Mouse PBMC Isolation Kit (Solarbio ...
• Targeted LNPs deliver mRNA encoding IL-15 superagonists to balance efficacy and toxicity in cancer therapy

  Juntao Yu, et al., bioRxiv - Bioengineering 2024

  Quote: ... Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA), 96-well ELISA plates (Corning ...
• Targeted LNPs deliver mRNA encoding IL-15 superagonists to balance efficacy and toxicity in cancer therapy

  Juntao Yu, et al., bioRxiv - Bioengineering 2024

  Quote: ... Human IFN-gamma Quantikine ELISA Kit (R&D systems, MN, USA), Human IL-6 Quantikine ELISA Kit (R&D systems ...
• Gemcitabine and ATR inhibitors synergize to kill PDAC cells by blocking DNA damage response

  Stefanie Höfer, et al., bioRxiv - Cancer Biology 2024

  Quote: ... with the exception of human epidermal growth factor (R&D Systems), bovine pituitary extract (Gibco ...
• IL-8 Instructs Macrophage Identity in Lateral Ventricle Contacting Glioblastoma

  Stephanie Medina, et al., bioRxiv - Cancer Biology 2024

  Quote: The Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) was used to measure the secretion of 105 different soluble factors and cytokines in GBM tumor conditioned media ...
• A Role for Astrocytic Insulin-Like Growth Factor I Receptors in the Response to Ischemic Insult

  K. Suda, et al., bioRxiv - Neuroscience 2023

  Quote: ... Human IGF-I Quantikine ELISA Kit from R&D systems (DG100B) was used to measure IGF-I in serum ...
• Naïve T lymphocytes chemotax to CCL21 but not to S1P-rich serum

  Nicolas Garcia-Seyda, et al., bioRxiv - Biophysics 2023

  Quote: ... either with 10 μg/mL human ICAM-1 (R&D Systems), 1 μg/mL CCL21 (Miltenyi biotech ...
• EHD1-dependent traffic of IGF-1 receptor to the cell surface is essential for Ewing sarcoma tumorigenesis and metastasis

  Sukanya Chakraborty, et al., bioRxiv - Cancer Biology 2023

  Quote: The Human Phospho-RTK Array Kit from R&D systems (#ARY001B) was used ...