Labshake search
Citations for R&D Systems :
1501 - 1550 of 1921 citations for Rat IGF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their co-incubation with T2 cells pulsed with MART-126–35 (commercially synthesized and purified [>95%] by reverse-phase HPLC by Biomer Technology, Cheshire, UK) by ELISA (R&D Systems, Minneapolis, MN, USA). The MART-126–35–-reactive clones were further expanded in a second-round of exposure to 30 ng/ml anti-CD3 ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50 ...
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bioRxiv - Cell Biology 2021Quote: We performed a Phage-Fab (antigen-binding fragment) selection using a naïve Fab library (libF46) on an immobilized recombinant rat Unc5B-ECD Fc fusion protein (R&D systems). Phage particles fused with Fabs were incubated with an unrelated protein (e.g ...
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: CCL-149 rat lung epithelial cells (ATCC) were cultured in DMEM/F12 with 10% fetal bovine serum (FBS, R&D Systems) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Developmental Biology 2021Quote: ... MSD standard-bind microtitre plates were first coated with 30µl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 µg/ml in PBS ...
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bioRxiv - Neuroscience 2021Quote: Single-cell RNA-seq analysis of cultured NSCs isolated from the cortex of E14.5 Sprague-Dawley rats (NSC001, R&D systems; cultured according to the manufactures’ protocol ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated overnight with unconjugated goat anti-mouse/rat CD47 N-terminal IgV-like extracellular domain antibody (R&D Systems), biotin-conjugated anti-mouse CD55 REAfinity antibody (clone REA300 ...
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bioRxiv - Genomics 2023Quote: ... anti-FcγRII/III (rat anti-mouse, clone 190909, cat. MAB1460), and anti-FcγRI (rat anti-mouse, clone 29035, cat. MAB2074) were purchased from R&D systems. 40μg of anti-FcγRII/III and 20μg of anti-FcγRI was used in FcγR-blocking conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin, 1:400 for IF) was from R&D System (#AF3235). Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain ...
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bioRxiv - Neuroscience 2019Quote: ... The TNF-α concentration in the supernatant was quantified using the Duoset® enzyme-linked immunosorbent assay (ELISA) development system (R&D Systems, Abingdon, Oxfordshire, UK). Three wells for each CSF were used to estimate variation in the experiments ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... IFN-γ concentrations in cell-free supernatants were determined by Human IFN-γ DuoSet enzyme-linked immunosorbent assay (ELISA; DY285; R&D systems, Minneapolis, MN, USA). EYFP−/EBFP+ dLP-CHO cells with no anti-hPD1 payload circuit were used as the control cells.
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bioRxiv - Immunology 2020Quote: IL-1β and CXCL9 were measured using ELISA (IL-1β from cell culture supernatants: MLB00C, R&D Systems; IL-1β from serum: MHSLB00, R&D Systems; CXCL9: DY492, R&D Systems), G-CSF ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of IL33 and periostin in culture supernatants were measured by ELISA according to the manufacturer’s instructions (Invitrogen, cat #: BMS2048TEN, Carlsbad, CA, USA; R&D Systems, cat #: DY3548B, Minneapolis, MN, USA) and as described previously [10] ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then treated with 200ng/mL of recombinant rat NogoA (1026-1090aa) and Fc Chimera protein (Nogo-66; R&D Systems), or with human NogoA (566-748aa ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend]; goat anti-myeloperoxidase [goatMPO, AF3667, R&D Systems] ...
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bioRxiv - Immunology 2020Quote: ... 1.25% of supernatant from Con-A-stimulated rat splenocytes (assay positive control), c) recombinant canine IL-4 (rcaIL-4, 360 ng/mL) (R&D Systems), d ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Immunology 2020Quote: ... Quantitation of IL-2 muteins in mouse serum was performed using an electrochemiluminescent immunoassay with a biotinylated rat anti-mouse IL-2 (JES6-1A12, R&D Systems) as a capture reagent and a ruthenylated goat anti-mouse IgG ...
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bioRxiv - Cell Biology 2021Quote: LECs were pre-cultured with FBS-free and supplement-free basal medium for 24 h and then treated with medium containing 100 ng/mL of recombinant rat CTGF (R&D Systems) for 5 min ...
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bioRxiv - Bioengineering 2020Quote: Surface expression of decoy constructs on engineered MSC lines was assessed by using either APC-conjugated rat-anti-mouse gp130 (IL6ST) antibodies (clone FAB4681A, R&D Systems) or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1 ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 0.1% FBS (Hyclone Laboratories Inc.)] and stimulated with 10 ng/mL rat PDGF-AA ligand (R&D Systems, Minneapolis, MN, USA) diluted from a 1.5 μg/mL working solution in 40 nM HCl containing 0.1% BSA for the indicated length of time ...
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bioRxiv - Physiology 2021Quote: The concentration of pro-collagen 1α1 concentration in the peritendinous space was determined at select time points after amino acid consumption using a DuoSet® ELISA from R&D Systems (DY6220-05, Minneapolis, MN). Due to the large dialysate volume needed for the pro-collagen assay ...
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bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
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bioRxiv - Immunology 2023Quote: ... T cells were analyzed using ELISpot according to the manufacturer’s protocol ELISpot Rat interferon-gamma (IFNγ) (Cat: EL585 by R&D Systems, United States).
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bioRxiv - Cancer Biology 2024Quote: ... Sections from formalin-fixed paraffin-wax embedded tumours were stained with primary rat anti-mouse monoclonal anti-CD8 antibody (eBioscience, 4Sm15as) and goat anti-mouse polyclonal anti-PD-L1 antibody (R&D systems, AF1019). Slides were scanned and imaged using Hamamatsu Nanozoomer (Hamamatsu Photonics).
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bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
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bioRxiv - Microbiology 2022Quote: Secretion of CXC motif chemokine ligand 10 (CXCL10/IP-10, DY266) and CC chemokine ligand 5 (CCL5/RANTES, DY278) were quantified by DuoSet® ELISA (R&D systems, Bio-Techne GmbH, Wiesbaden, Germany) under BSL-3 conditions according to the manufacturer’s instructions using the appropriate ancillary reagent kit (DY007).
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bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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bioRxiv - Immunology 2020Quote: IL-1β and CXCL9 were measured using ELISA (IL-1β from cell culture supernatants: MLB00C, R&D Systems; IL-1β from serum: MHSLB00, R&D Systems; CXCL9: DY492, R&D Systems), G-CSF ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and incubated with antibodies against Kit (1:200 anti Kit AF1356 R&D Systems). The antigen-bound primary antibodies were detected using 1:500 dilution of biotinylated anti-goat IgG antibody (BA-5000-1.5 ...
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bioRxiv - Genomics 2021Quote: ... Kit protein was detected with 1:2000 anti c-kit (AF1356 R&D Systems) and 1:2000 of anti-goat secondary antibody (sc2020 ...
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bioRxiv - Microbiology 2024Quote: ... 16-plex kit (R&D systems) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The Proteome Profiler Human Phospho-RTK array kit and human phospho-kinase antibody array kit (R&D Systems) were used according to the manufacturer’s instructions ...