Labshake search
Citations for R&D Systems :
1501 - 1550 of 1663 citations for PD L1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Neutrophil specific elastase was quantified using the Mouse Neutrophil Elastase/ELA2 DuoSet ELISA kit and the DuoSet Ancillary Reagent Kit2 (both from R&D Systems). Cecal pathology was scored at necropsy ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then washed with FACS buffer and labeled with 1 µl/well of phycoerythrin-conjugated anti-mouse secondary (F0102B, R&D Systems) 1 hour at 4 °C ...
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bioRxiv - Immunology 2022Quote: Llamas were immunized intramuscularly once per week over six weeks with carrier free recombinant human IL-9 or mouse IL-9 (R&D Systems). Each llama (4 in total ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Physiology 2023Quote: Circulating TNFR1 and TNFR2 were measured in human or mouse samples using enzyme-linked immunosorbent assay (ELISA) kits (DY255, DY726, DY425 and DY426 respectively, all from R&D Systems) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of CXCL1 or CXCL2 protein in the supernatant was determined using a mouse CXCL1 or CXCL2 specific ELISA kit (R&D system). All experiments were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively, R&D Systems). Mouse IL-33 was quantified by ELISA Kit (Cat# 88-7333-22 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Naïve T cells were isolated by negative selection using a Mouse Naive T Cell CD4+/CD62L+/CD44low Column Kit (R&D Systems). Naïve CD4 T cells were treated with fluorescein isothiocyanate-labeled (FITC ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Bioengineering 2024Quote: ... we used both mouse (111 analytes) and human (105 analytes) proteome profiler XL kits (R&D Systems, Cat No. ARY028 and ARY022B). We screened the cell culture supernatant from Day 7 spheroid cultures with and without SIS ECM ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Bioengineering 2023Quote: ... labeled with Alexa Fluor 488, Cat. #IC7619G) and anti-E-Cad (E- cadherin, Cat. #MAB18382, from mouse) were obtained from R&D systems. Anti-Vim (Vimentin ...
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bioRxiv - Bioengineering 2023Quote: ... was used to measure the concentration of IL-1ra in vitro using a mouse IL-1Ra DuoSet ELISA kit (DY480; R&D Systems) with a 1:10 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... An equivalent amount of CM (1.0 ml for each condition) was incubated with each nitrocellulose membrane provided in the Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems). Membranes were developed following the instructions of the manufacturer.
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bioRxiv - Molecular Biology 2024Quote: Total CCL2 and CCL17 concentration in the enriched cell free BALF was calculated using mouse CCL2 and CCL17 DuoSet Elisa (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... ELISAs were performed to quantify IL-1β in these culture supernatants using the Mouse IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) per the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... levels were analyzed in serum using the mouse GDF-15 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems DY6385).
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 2 h at room temperature with secondary antibodies in TBS-T containing 10% milk: anti-Mouse IgG (HAF018, R&D Systems) and anti-Rabbit IgG (A9196 ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Immunology 2024Quote: ... IL-1β and IFN-β were measured using mouse R&D Duo set ELISA kits according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Developmental Biology 2024Quote: Cells in 60 wells were incubated with primary goat polyclonal anti-mouse E-cadherin (R&D Systems, Cat. No AF748, 1:200) in cold 5% FBS/PBS for 20 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
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bioRxiv - Molecular Biology 2020Quote: L-FABP levels in human and mouse sera were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... and tubulin were detected by immunoblotting using HIV-1 gag p24 antibody (1:1000 dilution, MAB7360, mouse monoclonal, R&D systems, Minneapolis, MN), human CD207 antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C in buffer G with the following antibodies: mouse monoclonal anti-Tuj1 (R&D Systems, MAB1195) at 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... The cytokines released from macrophages treated with hemin were detected by mouse cytokine proteome profiler array panel A kit (ARY006, R&D Systems, USA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The TNF-alpha levels in the primary microglial supernatants were quantified using Quantikine® Mouse TNF-alpha ELISA kit (R&D Systems, MTA000B), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: Cytokine and chemokine levels were measured in the basal outflow of mouse intestine chips using a custom MSD U-plex Assay (Meso Scale Diognostics) and the mouse CXCL1/KC DuoSet ELISA (R&D Systems DY453) according to the manufacturers’ instructions.
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
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bioRxiv - Cell Biology 2021Quote: ... intracellular staining was done using the permeabilization buffer in the FIX & PERM kit with PE-conjugated anti-mouse α-SMA (clone: 1A4, R&D Systems) at 1:20 in the permeabilization buffer ...
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bioRxiv - Microbiology 2020Quote: ... and visualized on a Wes system (ProteinSimple, San Jose, CA, USA) with an anti-mouse or anti-goat IgG-HRP antibody (R&D systems, HAF109) detecting the primary antibody against GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Concentrations of IL-6 and IL-1β were measured using the Quantikine Mouse IL-6 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and Quantikine Mouse IL-1β ELISA Kit (R&D Systems) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1, 1:50,000, R&D Systems – AF3787). After 2x10 minutes washes with milliQ water and 2x10 minutes washes with TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies consisted in the human anti-spike mAb 48 (1:1,000; a gift from H. Mouquet) or the mouse anti-p24 Gag MAB7360 (R&D Systems; 1:1000). Anti-human or mouse IgG secondary antibodies ...
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bioRxiv - Pathology 2020Quote: ... IGF-1 was measured in the samples using a R&D Systems Mouse 1-Plex Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was immediately used to determine the levels of IL1β and IL6 using Quantikine Mouse ELISA kits and following the manufacturer’s protocols (R&D Systems, Minneapolis, Mn).
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Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)bioRxiv - Biochemistry 2021Quote: ... We collected the cell culture supernatant and measured the TNF-α concentration using Mouse TNF-α Quantikine ELISA Kit MTA00B (R&D SYSTEMS).
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bioRxiv - Immunology 2020Quote: ... except for the STORM experiments where they were used at a concentration of 20 μg/ml: α-MerTK (mouse extracellular monoclonal, 125618, R&D Systems), α-MerTK (goat extracellular polyclonal ...
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bioRxiv - Immunology 2021Quote: ... Each pooled sample was assayed for 40 different cytokines using the Proteome Profiler Mouse Cytokine Array Panel A kit according to manufacturer’s instructions (R&D Systems, Minneapolis, MN). Membranes were imaged using a LI-COR Odyssey Fc chemiluminescence imager (Lincoln ...
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bioRxiv - Immunology 2021Quote: ... Primary antibody (Anti-mouse RAE-1 pan-specific antibody, recognizing RAE-1α, β, δ, γ and ε (R&D Systems, Catalogue Number AF1136) or an appropriate isotype-matched control (polyclonal goat isotype IgG ...
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bioRxiv - Cell Biology 2021Quote: ELISAs were performed on sera collected from mice at terminal bleed with the following kits per manufacturers’ instructions: Mouse IL-1β/IL-1F2 (R&D Systems, MLB00C), mouse TNF-alpha (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647, clone H398, lot 0410; or APC-labelled anti-mouse gp130, R&D Systems, cat #FAB4681A ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNγ released into the supernatant by CAR-T cells was then measured using the DuoSet ELISA kit for mouse IFNγ (R&D systems, DY485) and Nunc MaxiSorp flat bottom plates (Thermo Fisher Scientific ...