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Citations for R&D Systems :
1501 - 1550 of 1766 citations for IFN gamma Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: The cytokine profile was analysed according to the manufacturer’s instructions (Mouse Cytokine Array Panel A Kit, R&D Systems, Catalogue #: ARY006). In these assays ...
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bioRxiv - Cell Biology 2023Quote: Isolated CD8+ DCs were cultured in R10 media supplemented with recombinant mouse FLT3L (250ng/ml; R&D Systems, 427-FL-025). CD8+ DCs isolated from Rubicon-proficient mice were incubated in the presence or absence of 5μM Fendiline-HCl (Tocris ...
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bioRxiv - Microbiology 2023Quote: The protein level of LCN-2 was analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems). The protein level of TNFα was measured using the Mouse TNFα ELISA kit following the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the amount of interleukin (IL)-4 protein was evaluated using a Mouse IL-4 Quantikine ELISA Kit (R&D Systems). Change in body weight was calculated by the difference between the day of intrarectal oxazolone administration and the end of study.
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bioRxiv - Neuroscience 2024Quote: ... Plasma IL-6 levels were then determined using a commercially available mouse IL-6 Quantikine ELISA Kit (R&D Systems, M6000B). 5-6 mice per group (saline and LPS ...
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bioRxiv - Physiology 2024Quote: ... supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100). BCA assay was performed to normalize IGF-1 concentration to protein concentration in the liver ...
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Cell Biology 2019Quote: Culture supernatants from NIH-3T3 cells treated with RSL3 for 24 hours were analyzed for secreted cytokines/chemokines using Proteome Profiler Mouse XL Cytokine Array (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg total protein was collected from each treated kidney and IL6 protein level was determined by the mouse IL6 duo-set (R&D Systems) ELISA ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Genetics 2021Quote: ... Four Ctsc mutations were propagated in separate intercross mouse lines and were validated as null mutations by Western Blot using an antibody against CTSC (Catalog #AF1034; R&D Systems, Inc. ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... diluted 1:200 and analyzed for adiponectin by ELISA following the manufacturer’s instructions (Mouse Adiponectin/Acrp30 Quantikine ELISA Kit, R&D Systems, MRP300). Absorbance (450 nM ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used for immunofluorescence in this study were as follows: anti-mouse 1:150 GPR34 (R&D Systems; Cat. No. MAB4617), anti-rabbit 1:1000 IBA1 (Wako ...
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bioRxiv - Molecular Biology 2021Quote: ... Cytokines and chemokines were screened using an array kit (Proteome Profiler Mouse Cytokine Array Kit, R&D Systems, McKinley Place, MN, USA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... the vessels were incubated with either PBS or different concentrations of recombinant mouse CXCL9 protein (R&D systems, catalog number 492-MM) for 3-4 hrs ...
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bioRxiv - Pathology 2019Quote: Analysis of the cytokine proteome was completed using a Mouse XL Cytokine Array Kit (R&D Systems, Bio-Techne Corporation, MN, USA). Gingiva and alveolar bone samples were individually pooled ...
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Immunology 2019Quote: ... IL-17 was detected using goat anti-mouse IL-17 (1/100 dilution, Catalogue number Af-421-na R&D Systems, UK) followed by biotinylated rabbit anti-goat IgG (1/200 dilution ...
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bioRxiv - Physiology 2020Quote: BALF was analyzed using a DuoSet ELISA kit for Mouse CCL2/JE/MCP-1 and TGF-β1 (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions using a SpectraMax M5 Microplate Reader (Molecular Devices ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Microbiology 2021Quote: IL-1β and IL-6 were detected in tissue lysate using the Mouse IL-1β and IL-6 DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Microbiology 2021Quote: ... IL-1β (cat No. DY401) and IL-12 p70 (cat No. DY419) by using the mouse DuoSet ELISA system (R&D Systems). In addition ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were fixed in an ice-cold acetone/methanol mixture (50%/50%) and stained with primary anti-mouse CD144 antibody (MAB9381, 1:100, R&D Systems) and secondary donkey-anti-mouse Alexa Fluor® 594 (A21203 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human ACE2 polyclonal antibody (AF933) and mouse anti-human ACE2 mAb MAB9332 were available from R&D Systems (Minneapolis, MA). Rabbit anti-SARS-CoV-2 RBD (40592-T62 ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of secreted IL-33 in the conditioned media was visualized on a Proteome Profiler Mouse XL Cytokine Array (R&D Systems). The concentration of IL-33 secreted in the conditioned medium was measured on a V-PLEX Mouse Cytokine 19-Plex Kit (Meso Scale Diagnostics ...
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bioRxiv - Microbiology 2019Quote: ... BSA (24 - 48 h at 4°C in rotation) for HIS-tagged rPtxS1 and rGαi proteins with mouse monoclonal anti-HIS (1:1000) (R&D Systems, MAB050), for Gαi with mouse monoclonal anti-Gαi (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... The relative level of cytokines within the culture supernatant was determined using the Proteome Profiler Mouse Cytokine Array (R&D System, Minneapolis), as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Immunology 2022Quote: CD4+ splenic T-lymphocytes were polarized to TH17 ex vivo by use of CellXVivo Mouse Th17 Cell Differentiation Kit (#CDK017, R&D Systems). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of LCN-2 in the supernatant was measured by using a DuoSet Mouse Lipocalin-2/NGAL ELISA (R&D Systems) kit ...
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bioRxiv - Immunology 2020Quote: TGFβ-1 concentration in supernatant from 4day ILC1 co-cultures and SIO only controls was measured using the Mouse TGFβ-1 DuoSet ELISA (R&D Systems) with modified manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Synovial tissue sections were stained using antibodies against CXCL12 (Monoclonal Mouse IgG1, Clone #79018,Catalog Number: MAB350, R&D Systems, Abingdon, UK), CXCR4 (Monoclonal Mouse IgG2B ...
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bioRxiv - Microbiology 2020Quote: Levels of circulating TNF-α in infected mice were measured using Mouse TNF-α Immunoassay Quantikine® ELISA kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were analyzed for lipocalin-2 content using Quantikine ELISA kit for mouse Lipocalin-2/NGAL (R&D Systems, Cat: MLCN20).
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bioRxiv - Cell Biology 2021Quote: ... sections were blocked with 10% rabbit serum for 15min at room temperature and then incubated with goat anti mouse IGF1(1:40, AF791, R&D System) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... All cell lines were routinely evaluated on a Flow Cytometer (Becton Dickinson, FACScan) for the expression of CD marker using anti-hCD3ε (PE Conjugated Mouse IgG1, R&D Systems) antibody to authenticate the cell line.
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Cell Biology 2022Quote: The concentration of active TGF-β1 was measured using the Human /Mouse /Rat /Porcine /Canine TGF-beta 1 Quantikine ELISA kit (DB100B, R&D Systems). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... then stained with 0.1 µg/sample Alexa Fluor 647-conjugated mouse-anti SARS-CoV-2 Spike S1 (R&D Systems, FAB105403R-100UG) at 4°C for 30 min in the dark ...
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bioRxiv - Pathology 2022Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The secondary goat F(ab’)2 anti-mouse IgG-PE antibody used for flow cytometry was purchased from R&D Systems (F0102B) (Minneapolis ...
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bioRxiv - Pathology 2020Quote: ... recombinant human Cadherin 6 Fc chimera protein (~130 kD) or recombinant mouse Cadherin 6 protein (~90 kD) was used to generate a standard curve (R&D Systems catalog # 2715-CA or 6826-CA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... we investigated levels of fecal calprotectin in the Il11SMC and Il11Fib mice using the mouse S100A8/A9 heterodimer duoset ELISA kit (DY8596-05, R&D systems). Calprotectin is a biomarker for inflammatory activity and has been clinically applied as a diagnostic tool for inflammatory bowel diseases [35,36] ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Myc-tagged and V5-tagged CARs were stained using an Alexa Fluor 488-conjugated mouse anti-myc antibody (R&D Systems, IC3696G) and an Alexa Fluor 647-conjugated mouse anti-V5 antibody (Invitrogen ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... The following antibodies were purchased and used for immunostaining of human sample using staining protocols similar to those used in mouse sections: CD31 (AF806, R&D systems), CXCR4 (2B11 ...