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Citations for R&D Systems :
1501 - 1550 of 1992 citations for Follicle stimulating hormone FSH Human HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: Human TNFα was quantified in blood serum (1:10 dilution) using a DuoSet ELISA kit (R&D Systems, Minneapolis, MN). Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution ...
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bioRxiv - Immunology 2019Quote: ... following relevant treatment with or without 200 U/ml of recombinant human (h) or mouse (m) TNFα (R&D Systems). Protein concentration was measured using the Bradford assay (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Biophysics 2019Quote: ... Ibidi channels IV0.4 (Clinisciences) were coated overnight at 4°C with 10μg/ml human ICAM-l-Fc (R&D Systems) in Phosphate Buffer Solution (PBS ...
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bioRxiv - Immunology 2021Quote: ... IL-11 was quantified by using a Human IL-11 Quantikine ELISA Kit according to manufacturer instructions (R&D Systems). Statistical analysis was performed on log10-transformed values with two-way ANOVA tests and Tukey correction ...
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bioRxiv - Neuroscience 2020Quote: ... During the first 10min, sections were superfused with either vehicle (i.e., 0.1%BSA/1xDPBS) or 100nM human SEMA3A Fc chimera (R&D System) diluted in warm (37°C ...
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bioRxiv - Bioengineering 2020Quote: ... IL-15 superagonist was quantified using the human IL-15/IL-15R alpha complex DuoSet ELISA (R&D Systems DY6924). For BiTE experiments with primary human T cells ...
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bioRxiv - Neuroscience 2020Quote: ... cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared and analyzed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, cat# ARY003B) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Phospho-kinase screening was performed using a phospho-kinase array (Proteome Profiler Human Phospho-Kinase Array #ARY003B; R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Physiology 2021Quote: ... CCL2 levels in urine specimens were quantified using Human CCL2/MCP-1 Quantikine® ELISA Kit (R&D systems, DCP00) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... was used as an invasion promoter and recombinant human nodal (250 ng/mL; R&D Systems, 3218-ND-025/CF) was used as an invasion inhibitor ...
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bioRxiv - Cell Biology 2022Quote: Cells were characterised using hPSC imaging kit (Human Pluripotent Stem Cell Marker Antibody Panel kit, SC008, R&D system, UK) with analysis performed on a flow cytometer (Beckman Coulter Cytomics FC 500) ...
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bioRxiv - Immunology 2022Quote: ... The concentration of samples was extrapolated from standard curves of recombinant human IL7R-Fc chimera (R&D Systems, # 306-IR).
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
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bioRxiv - Cancer Biology 2022Quote: ... 104 sorted CD34+ HSPCs were plated for methylcellulose-based colony forming assays [Human Methylcellulose Base Medium HSC002 (R&D Systems), which were supplemented with 2% streptomycin/penicillin ...
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bioRxiv - Microbiology 2021Quote: ... ELISA was performed using Human Gas6 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Minneapolis, Minnesota, USA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Cancer Biology 2022Quote: ... Chemokine levels were measured using the Invitrogen ELISA Kit (Quantikine mouse/human CXCL10/IP-10; R&D Systems, Minneapolis, MN) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... with mouse and human soluble FLT1-FC chimeric protein (471-F1-100 and 321-FL-050/CF, R&D Systems) immobilized to about 1,000 RU was used as ligand for the analyte binding of anti-FLT1 MAbs ...
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bioRxiv - Cancer Biology 2020Quote: ... Prostate-specific serum-free culture media contained 500 ng/mL human recombinant R-spondin (R&D Systems, Cat: 10820-904), 10uM SB202190 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718, RRID:AB_2278695; R&D Systems, Inc., Minneapolis, MN, USA); and developed using SuperSignalTM west -pico or -femto chemiluminescence kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human WNT3A (#5036-WN-010) and WNT4 (#6076-WN-005) were obtained from R&D Systems (Minneapolis, MN, USA) and reconstituted per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2021Quote: ... the concentration of amphiregulin in the supernatant was determined using the Human Amphiregulin Quantikine ELISA Kit (R&D Systems, DAR00) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Genetics 2020Quote: Plasma VEGF-A concentration was measured using the quantitative sandwich ELISA kit (Human VEGF Quantikine; R&D Systems, Minneapolis, MN) following the manufacturer’s instructions in our laboratory at low altitude (600 m) ...
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bioRxiv - Immunology 2022Quote: ... immune mediators in cell supernatants were measured using Human Custom 29-plex Multi-Analyte Kit (R&D Systems, Minneapolis, Minnesota) measuring the following analytes ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Immunology 2022Quote: IL-22BP quantification was performed with the Human IL-22BP ELISA DuoSet kit (R&D System, ref DY-1087-05) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Microbiology 2024Quote: Recombinant human IFN-β (8499-IF) and IFN-λ2 (1587-IL) were purchased from R&D Systems (Minneapolis, MN, USA). Both were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: For Wnt5a experiments tetracycline induced GFP-RasV12 cells were treated with 100ng/mL recombinant human/mouse Wnt5a (R&D Systems) or PBS (control-Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were flooded with BrainPhys media with supplements and 10 ng/mL human NGF (256-GF-100, R&D Systems), and 3 μg/mL FRDU and incubated at 37° C and 5% CO2 until further experimentation.
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Immunology 2024Quote: The chemokine CXCL13 was measured in LO chip effluent using a human CXCL13 ELISA assay (Quantikine, R&D Systems #DY801), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
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bioRxiv - Biochemistry 2024Quote: The human cell line H1299 was acquired from ATCC and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... IFN-β amounts in the culture supernatant were measured by the human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Pathology 2023Quote: CCL2 was quantified in conditioned media from HAoSMCs using Human CCL2/MCP-1 Quantikine ELISA Kit (DCP00, R&D systems) according to the manufacture’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.