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Citations for R&D Systems :
1451 - 1500 of 3689 citations for 7 Chloro 2 hydrazino 5 phenyl 3H 1 4 benzodiazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary goat F(ab’)2 anti-mouse IgG-PE antibody used for flow cytometry was purchased from R&D Systems (F0102B) (Minneapolis ...
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bioRxiv - Pathology 2020Quote: ... was measured immediately before injection and at 3 days after injection using a Mouse Lipocalin-2/NGAL Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems) to ensure successful injection of folic acid ...
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bioRxiv - Immunology 2020Quote: ... Quantitation of IL-2 muteins in mouse serum was performed using an electrochemiluminescent immunoassay with a biotinylated rat anti-mouse IL-2 (JES6-1A12, R&D Systems) as a capture reagent and a ruthenylated goat anti-mouse IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... and then differentiated into day 2 definitive endoderm through the addition of CDM2 basal medium supplemented with Activin A (100 ng/mL; R&D Systems), LDN-193189 (250 nM ...
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bioRxiv - Immunology 2021Quote: Plasma cytokine and chemokine profiles from Mif-2−/−Apoe−/− mice and Apoe−/− mice were mapped by capitalizing on a membrane-based mouse cytokine array panel A (R&D Systems) following the standard instructions ...
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bioRxiv - Pathology 2021Quote: Frozen maternal serum aliquots were quick thawed and immediately assayed using either a Human ACE-2 DuoSet ELISA assay (R&D systems) or a ParameterTM Estradiol assay per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-21 and IL-15 were measured in plasma diluted 1:2 from acute COVID-19 patients and acute healthy controls using a custom designed multiplex Magnetic Luminex Screening assay (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Cancer Biology 2021Quote: Human gastric and colon organoids were generated from deidentified surgical specimens from Stanford Hospital under an approved IRB protocol following established methods and grown in a submerged format in WENR media (Matano et al., 2015; Sato et al., 2011) in BME-2 extracellular matrix (R&D Systems) (Lo et al. ...
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Bioengineering 2024Quote: ... Enzyme-linked immunosorbent assays (ELISA) was then carried out as per manufacturer’s instructions (R&D Systems, BMP-2 DuoSet ELISA kit, DY355). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 2 h at room temperature with secondary antibodies in TBS-T containing 10% milk: anti-Mouse IgG (HAF018, R&D Systems) and anti-Rabbit IgG (A9196 ...
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bioRxiv - Immunology 2023Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of rhuIL-2 (10 U/mL, R&D System) to expand the T cells ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... TSP2 concentrations in LX-2 cell lysates were determined using enzyme-linked immunosorbent assays following the manufacturer’s instructions (Quantikine® ELISA, #DTSP20, R&D Systems) (16) ...
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bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
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bioRxiv - Immunology 2023Quote: ... Unpurified transient transfection supernatant samples (50 µl) were pre-incubated with biotinylated SARS-CoV-2 spike protein (R&D Systems cat# BT10549) (50 µl ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Bioengineering 2024Quote: ... CAR T cells for the initial challenge day 0 imaging experiment were stained with the following antibody mix in FACS buffer (PBS 1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... cells from re-challenge plates were stained with the following antibody mix in FACS buffer (PBS1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Immunology 2024Quote: ... then maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems). For the pilot experiment in which CD4 cells with a defined CAR BC were spiked into CD8 cells (Figure 1D-F) ...
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bioRxiv - Immunology 2024Quote: ... All primary cells were maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems) at 37°C in 5% CO2.
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bioRxiv - Developmental Biology 2021Quote: ... in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin) with added FGF ligands (Fgf7; 4 µM, R&D Systems, Cat. No. 5028-KG-025), the γ-secretase inhibitor DAPT (10 µM ...
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bioRxiv - Immunology 2022Quote: ... immobilized hIgG, or 50 ng/ml recombinant GM-CSF (rGM-CSF) (Bioscience, US) plus 20 ng/ml IL-4 (R&D systems Cat.No. 204-IL-010, US), for 5 days to obtain immature dendritic cells (iDC) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the DE monolayer was incubated for 4 days with the hindgut induction medium supplemented with 500 ng/mL of FGF4 (R&D Systems, cat # 235-F4-025/CF) and 3μM of CHIR99021 (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Goat anti-Dll4 (*with antigen retrieval 1:50 and without antigen retrieval 1:25, AF1389, R&D Systems), *Rabbit anti-NICD activated (1:75 ...
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bioRxiv - Neuroscience 2022Quote: ... 1% P/S and 0.l l⍰lμ gl⍰lml-1 of interleukin (IL)-34 (R&D Systems) and 0.01 μg ml −1 of granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were stained with TRA-1-60 conjugated FITC antibody (R&D system, FAB4770G, 1:50 dilution) or normal IgM conjugated FITC (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies were as follows: goat anti-Lyve-1 (R&D Systems, Minneapolis, MN, USA; 1:600), rabbit anti-Prox-1 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Chicken anti–GFP (GFP10-20; AVES, 1:2000) Goat anti–mouse podocalyxin (AF1556; R&D Systems, 1:1000) Rabbit anti–mouse LYVE-1 (11-034 ...
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bioRxiv - Microbiology 2022Quote: ... we changed the RSPO-1 conditioned media to lyophilized RSPO-1 (80ng/ml) (R&D systems 4645-RS).
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bioRxiv - Microbiology 2021Quote: ... cells were blocked with 1% BSA and stained overnight with goat anti-ACE2 (1:200, R&D Systems) in blocking buffer followed by rabbit anti-goat Alexa Fluor 488 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... ELISA for HO-1 was performed using a human HO-1 DuoSet IC kit from R&D Systems, and total protein was assessed using a PierceTM BCA Protein Assay Kit.
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies (Rabbit anti-MAP2, Millipore, AB5622, 1:800; Mouse anti-Tuj1, R&D Systems, mab1195, 1:500) were diluted in PBS and added to the wells to incubate overnight at 4°C while gently rocking ...
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bioRxiv - Biochemistry 2022Quote: ... then incubated with 1:1000 α-polySia mAb 735 (BioAspect) or 1:2000 α-NCAM (R&D Systems) in blocking buffer at room temperature overnight ...
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bioRxiv - Biophysics 2023Quote: ... The samples were then exposed to either 10 nM thrombospondin-1 (TSP-1) (3074-TH, R&D Systems) or HBSS for 10 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytokines released from HCEC-1CT cells into cell culture supernatants after 5 h of incubation were measured using a Magnetic Luminex® assay (R&D SYSTEMS, Minneapolis, USA). Cell culture supernatant samples were collected after the incubation for cytokine gene transcription (details for incubation see 2.4. ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... The B cells were then added to the APCs and cultured in complete medium with 5 ng/ml BAFF (R&D Systems, #8876-BF-010) and 5 ng/ml IL-4 (R&D Systems ...