Labshake search
Citations for R&D Systems :
1451 - 1500 of 3897 citations for 7 Bromo 5 methyl 1 2 4 benzotriazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... and goat anti-jagged 1 (1:150, AF599, R&D Systems, RRID: AB_2128257). Secondary antibodies (all raised in donkey ...
-
bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
-
bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293; anti-PTDGS/PGD2 R&D Systems CAT#MAB10099 1:100 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were blocked in PBS and Tween-20 with 1 % (w/v) nonfat dry milk for 1 h at RT followed by incubation with 1:50 PAX6 (AF8150; R&D Systems) or 1:500 FOXC1/2 (CPA3482 ...
-
bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
-
bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C in buffer G with the following antibodies: mouse monoclonal anti-Tuj1 (R&D Systems, MAB1195) at 1:1000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
-
bioRxiv - Physiology 2020Quote: ... non-esterified fatty acid (NEFA) serum levels were measured by colorimetric assay (Randox, UK) and dipeptidyl peptidase 4 (DPP4; R&D Systems, UK) levels were measured by ELISA.
-
bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
-
bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
-
bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
-
bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
-
bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
-
bioRxiv - Cell Biology 2019Quote: ... SKBr3 cells co-transfected with EGFP-α-tubulin and the indicated siRNA or cDNA were grown on collagen-coated glass coverslips for 48 h and observed 10 min after the addition of 5 nM HRGβ1 (R&D Systems) using the 63X objective (plan Apochromat NA 1.4 ...
-
bioRxiv - Bioengineering 2019Quote: ... HUVECs were encapsulated (5·106 cells/mL) in situ within hydrogels loaded with 200 pmol/mL VEGF-165 (R&D Systems) or within non-loaded hydrogels (without GF) ...
-
bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
-
bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
-
bioRxiv - Developmental Biology 2020Quote: ... 50 ng/mL FGF1 and 5 μg/mL heparin or 10 ng/mL PDGF-AA (R&D Systems #1055-AA-050) supplemented DMEM for 12 hrs.
-
bioRxiv - Developmental Biology 2020Quote: ... Micromass cultures were then maintained at 37°C in a humidified incubator with 5% CO2 for 24hrs with the inhibitors before treatment with 5ng TGFβ1/ml (R&D Systems) for the indicated times.
-
bioRxiv - Cancer Biology 2019Quote: ... Active CD73 enzyme (Recombinant Human 5’-Nucleotidase/CD73 Protein, CF) and active CD39 (His-Tag Human) were from R&D Systems, Minneapolis ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were treated with increasing doses of TGFβ2 (vehicle control, 1.25 ng/ml, 2.5 ng/ml, and 5 ng/ml; R&D Systems, Minneapolis, MN) for 48 h ...
-
bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
-
bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... 100 µg/ml streptomycin and either 5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Abingdon, UK) to promote M1-differentiation or 20 ng/ml macrophage colony-stimulating factor (M-CSF ...
-
bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
-
Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ICAM-1 and VCAM-1 protein levels were evaluated by SDS PAGE using goat anti-Icam-1 (R&D systems Cat# AF-796) and rabbit anti-Vcam-1 (abcam ...
-
bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP; hSPARCL1 1:500, R&D systems, #AF2728-SP ...
-
bioRxiv - Cell Biology 2020Quote: ... goat anti-AXL (AF154, IF 1:500, WB 1:1000) from R&D Systems; mouse anti-AKT (2920 ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-Sox2 (SC17320, Santa Cruz Biotechnology; 1:300; MAB2018, R&D Systems 1:50), anti-Sox9 (AF3075 ...
-
bioRxiv - Biochemistry 2022Quote: ... RhoA (1:500, NBP2-22529 NovusBio) and Klotho (1:1000, AF1819 R&D Systems). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase-conjugated secondary antibodies (Milian Analytica ...
-
bioRxiv - Cancer Biology 2021Quote: ... CCL27 (1:200, HIER 1, mouse monoclonal, clone 124308, R&D Systems, USA; MAB376), CCR10 (1:100 ...
-
bioRxiv - Cell Biology 2021Quote: ... Recombinant Mouse sFRP-1 Protein (1 µg/ml, R&D Systems, MN9019-SF-025), Plexin-D1 Fc fragment (30 µg/ml) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor (Thermo 1:200) and Northern Lights (R&D Systems 1:200) conjugated secondary antibodies were incubated for 1 hour at 37ºC ...
-
bioRxiv - Physiology 2023Quote: ... and Goat anti mouse CD31/PECAM-1 (AF3628-SP, 1:150, R&D Systems) diluted in blocking buffer ...
-
bioRxiv - Immunology 2021Quote: ... Dimeric IgA in BAL and nasal swabs was measured using DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) as described before20 ...
-
bioRxiv - Molecular Biology 2020Quote: ... FGF-2 in the CM was assayed using a human FGF basic DuoSet ELISA Development Kit (R&D Systems) according to the manufacturer’s procedure.
-
bioRxiv - Immunology 2021Quote: ... half-medium exchange was performed and 10 IU/mL IL-2 (R&D Systems; Cat no 202-IL-500) added ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
-
bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
-
bioRxiv - Immunology 2020Quote: ... 2-incubation with 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) in microscopy buffer for 1 h at RT ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... An ELISA for mouse lipocalin-2 was performed on feces (protocol according to the manufacturer; R&D Systems kit) to determine the inflammatory state of the gut.
-
bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...
-
bioRxiv - Microbiology 2024Quote: ... was measured from the resulting supernatant using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN). Measuring lipocalin-2 (Lcn-2 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with compounds for 2 hours prior to TGF-β1 stimulation (1ng/ml, R&D systems. After 48 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... Both PDO lines were maintained in growth factor reduced type 2 Cultrex (Biotechne/R&D Systems, 3533-010-02) with phenol red-free DMEM/F-12 ...