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Citations for R&D Systems :
1451 - 1500 of 1526 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Genetics 2023Quote: Reprogramming was done as described previously, combining the IFNγ (10 ng/ml, day 0 to 5) (R&D Systems, 485-MI-100) with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Biophysics 2023Quote: Collagen fibrils were formed by combining rat tail tendon-derived acid-soluble type I collagen (5 mg/mL in 20 mM acetic acid) (CultrexRat Collagen I, R&D Systems) with 400 mM HEPES + 0.02% sodium azide and 1 M NaCl at room temperature to reach final concentrations of 1 mg/mL collagen ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were incubated with anti-Siglec-9-APC (Clone 191240) and/or anti-Siglec-5/14-AF488 (Clone 194128) (R&D Systems) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: Human dermal fibroblasts transfected with either 10 nM si-circGLIS3 or scrambled siRNA (Eurofins Genomics, Ebersberg, Germany) for 48 hours and treated with 5 ng/ml TGF-β1 (R&D Systems) were rinsed and pelleted in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: The preosteoblast cells cultured in tissue culture plates were differentiated for 5 days and treated with Myostatin of 100 ng/mL (788-G8-010/CF R&D system), follistatin of 100 ng/mL (769-FS-025/CF ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then washed with phosphate-buffered saline (PBS)/0.05% Tween 20 and incubated with mouse IgG2a-anti-IAV NP (Clone Hb65, EVL laboratories) 5 μg/ml or mouse IgG2a isotype control (MAB003, R&D systems) 2.5 μg/ml in PBS/0.1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... cells were washed and split into two new 48-well plate wells and cultured with IL-2 (5 ng/ml; R&D Systems), in the presence or the absence of CH-223191 (10 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated in blocking solution (3% bovine serum albumin + 0.1% Triton X-100 + 5% Horse serum) at room temperature for 1hr and then with anti-OSMR beta (R&D Systems, AF662) antibody (10 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively, R&D Systems). Mouse IL-33 was quantified by ELISA Kit (Cat# 88-7333-22 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... bEFNB2 expressing CHO cells were passaged in puromycin (1-5 ug/mL) for 1 week and then stained with EphB3-Fc (5667-B3-050; R&D Systems), a natural ligand of EFNB2/3 ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice ...
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bioRxiv - Immunology 2024Quote: Fecal samples collected from female mice at 5 weeks of age were assayed for immunoglobulin and Lcn-2 content using ELISA kits (Mabtech; R&D Systems). Protein was extracted from fecal samples by manual homogenization of 2-3 pellets in PBS + 0.05% Tween20 followed by 4 hr of agitation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... in either 10% chicken sera in 5% BSA/PBS (immunofluorescence) and probed with goat anti-human MPO (detects mouse MPO, AF3667 R&D Systems) rat anti-mouse CD45 (all leukocytes ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of mitochondria (1μg/μl protein) were treated with Caspase-8-cleaved tBid from 5-500nM (882-B8-050, R&D Systems, Minneapolis, MN), Bim-BH3 from 10-10,000nM (Ac-DMRPEIWIAQELRRIGDEFNAYYARR-amide ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...
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bioRxiv - Immunology 2023Quote: ... 5 × 105 IHMCs were plated in each 96-well and incubated in 75µL complete R10 media with rhMIF (R&D Systems; 1mg/mL) or vehicle (PBS + 0.2% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatant was collected and centrifuged (3000 rpm, 5 min, RT) and Secreted IL6 was measured using the Quantikine® ELISA kit (R&D Systems), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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bioRxiv - Immunology 2022Quote: Substrate functionalization was performed by overnight incubation with 5 µg/ml CCL21 and 50 µg/ml ICAM1 (R&D Systems, Minneapolis, MN, USA), in PBS.
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Cell Biology 2023Quote: ... and tissue harvest as previously described and in Supplementary Methods.6 Structural analysis of lung tissue included hematoxylin and eosin stain as well as immunostain for muscularization of vessels as previously described.7 Cytokine levels were measured by pooling plasma and lung lysate from 5 mice treated with either L-NAME/HFD or control water/diet for 5 weeks and was assayed using an antibody-based cytokine array (R&D Systems, ARY006) and was completed according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were seeded in tissue culture-treated 12-well plates coated with human fibronectin (5 µg/cm2) (R&D Systems, Cat. # 1918-FN). Stimulated and unstimulated groups were formed as described above ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µL of a 5 µg mL-1 biotinylated polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution in PBS (R&D Systems, USA) was added to CellStudio substrates containing streptavidin coated microbeads patterns (500 nm diameter beads ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5% fetal Bovine serum (Hyclone, #SH300084) and treatment with 2.5 ng/ml of recombinant Human TGF-β 1 Protein (R&D systems, #RDS240B002) for maximum of 18 days ...
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bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were administered either a 5 μL volume of phosphate-buffered saline (PBS) containing 10 μg/kg of carrier-free rmIL-1β (R&D Systems, Minneapolis, MN) or PBS alone (control ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Cell Biology 2021Quote: ... media was replaced with RPMI supplemented with B27 without insulin and containing 1 µM CHIR99021 and 5 ng/ml BMP4 (R&D systems #314-BP). After an additional 48 hours (Day 3) ...
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bioRxiv - Cell Biology 2020Quote: ... were covered with HUVECs and embedded 24 hours later in a 2,5 mg/mL fibrin gel in the presence or absence of VEGFA-165 at 5 ng/mL (R&D systems, Minneapolis MN, USA). Conditioned media from papillary or reticular fibroblasts were added on top of the gel and renewed every day for four days ...
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bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Filters were blocked in 5% nonfat milk in TBS-Tween 20 (0.1%) and incubated overnight with rabbit anti-NrCAM (R&D Systems AF8538, 1:1000), mouse anti-Rac1 (BD Biosciences # 610651 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and HEK293T cells (ATCC) were cultured in DMEM (4.5 g/liter glucose, pH 7.2) with 5% FBS (Atlanta Biologicals/R&D Systems #S11150, Lot #F18071) 20 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...