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Citations for R&D Systems :
101 - 150 of 156 citations for Synapsin I derived peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Recombinant human TGFβ and Universal Type I IFN was obtained from R&D Systems.
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bioRxiv - Genetics 2019Quote: ... Cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005, R&D Systems) and stained with 1:100 of Rabbit anti-pAKT (S473 ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA from LAB or Poly (I: C) (4287/10, R&D Systems, USA) was transfected into the cells at a final concentration of 5 μg/ml and 1 μg/ml using Lipofectamine 2000 (11668027 ...
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bioRxiv - Cancer Biology 2023Quote: ... rat tail collagen type I was purchased from R&D Systems (Minneapolis, MN, USA) and glacial acetic acid was purchased from Fisher Scientific (Hampton ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse or rat collagen I (human, R&D Systems; mouse, Ray Biotech; rat, Yo Protein) or collagen III (human ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with 150 μg/mL Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA). Cells were cultured in endothelial base medium (EBM-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
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bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% BSA-containing DBPS in Cultrex rat Collagen I solution (R&D Systems, 3440-005-01) 170 ul was loaded in the center chamber ...
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bioRxiv - Developmental Biology 2021Quote: ... or snapshot acquisition after cell synchronization in metaphase using 60μM proTAME (R&D systems, #I-440-01M) and 200μM Apcin (R&D systems ...
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DIET INFLUENCES PERIPHERAL AMYLOID β METABOLISM: A ROLE FOR CIRCULATING INSULIN-LIKE GROWTH FACTOR IbioRxiv - Neuroscience 2020Quote: IGF-I in serum and brain was determined using a species-specific ELISA (R&D Systems, USA), as described in detail elsewhere (18) ...
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bioRxiv - Biochemistry 2022Quote: ... I used 10 ng/ m L recombinant human interleukin 1 (IL-1α; R&D Systems, Minneapolis, MN). All incubations were done at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...
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bioRxiv - Physiology 2022Quote: ... Proteasomal activity (FITC channel) was determined using Me4BodipyFL-Ahx3Leu3VS fluorescent proteasome activity probe (R&D Systems, I-190) at 2 μM for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... a GSC sphere was placed in NaOH-stabilized Cultrex rat collagen I (R&D Systems, 3440-005-01). To prevent incomplete and premature gelation of the collagen ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Verification of TIMP activity in vitro using activated MMP-2 and the fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 was performed as per the protocol provided by R&D Systems.
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Immunology 2021Quote: ... fifty microliters of stimulant pepmixes (JPT peptides) resuspended in RPMI+10%FBS (R10) with recombinant mouse IL-2 (rmIL-2) (R&D Systems) were added to each well ...
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bioRxiv - Microbiology 2023Quote: PLY-treated H292 monolayers or PMNs in suspension were treated with 1U PLY and incubated for 30 min at 37 °C with 10 μM fluorogenic peptide MMP substrate (Mca-PLAQAV-Dpa-RSSSR-NH2, R&D Systems) diluted in 25 mM Tris buffer ...
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bioRxiv - Pathology 2020Quote: ... the membranes were incubated with the sheep anti-human collagen I alpha 1 antibody (1:2000, R&D systems) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were washed in PBS and coated with 1 µg / mL laminin-I (R&D Systems; #3446-005-01) at 37 °C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: Microfluidic devices were prepared as described previously [32] using 2.5 mg/mL Collagen I (rat tail, R&D Systems) as extracellular matrix ...
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bioRxiv - Immunology 2023Quote: ... a 50:50 mixture of Matrigel Matrix GF and 4 mg/ml Cultrex Rat Collagen I (R&D Systems). For time-lapse live imaging experiments ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated in 0.5% serum-containing media before treatment with 200 ng/mL recombinant Shh N-terminal peptide (R&D Systems, 1314-SH) diluted in dimethyl formamide (DMF).
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λi>1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
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bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Biochemistry 2023Quote: ... were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37°C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Cancer Biology 2022Quote: PBMC (2×106 per well) were stimulated in 24-well plates with peptide (individual/pool) plus recombinant IL-2 (R&D Systems Europe Ltd.) at a final concentration of 5μg/mL and 20IU/mL ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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bioRxiv - Molecular Biology 2023Quote: ... and Fluorogenic Peptide Substrate VII: Z-Leu-Arg-4-methyl-7-coumarylamide (Z-LR-AMC)(Catalog # ES008) were purchased from R&D Systems (Minneapolis, MN, USA). The enzyme reactions were initiated upon addition of ice-cold of hrCTSL (96pM ...
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bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells were pre-treated for 16 h with Type I Interferon (1000 Units/mL; R&D Systems, 11200-2) and treated with HMW Poly(I:C ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were isolated from the spleens of female OT-I (CD45.1+/CD45.2+) mice using CD3+ T cell enrichment columns (R&D Systems, MTCC25) and co-cultured with the peptide-pulsed splenocytes for 3 days ...
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bioRxiv - Bioengineering 2024Quote: ... the platelets were washed twice in PBS and opsonized with anti-human HLA class I (R&D Systems, 2.5 µg/mL), IgG control (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... one ml solution of 250 ng/ml type I collagen with or without 250 ng/ml POSTN (R&D Systems, Minneapolis, MN) was added to each well followed by one-hour incubation at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Cell Biology 2021Quote: ... Spain) and supplemented with each one of the following combinations of growth factors: (i) 100 ng/ml Act A (R&D Systems, UK), (ii ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested after treatment and stained for PD-L1 (eBioscience, 12-5983-42) and MHC class I (R&D Systems, FAB7098G). Induction of the respective proteins was analyzed by flow cytometry.
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bioRxiv - Biophysics 2023Quote: ... and rTv20S-inhibitor complex were diluted with 50 mM HEPES pH 7.5 then mixed with 2 μM MV151 (R&D Systems #I-190). After MV151 addition ...