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Citations for R&D Systems :
101 - 150 of 704 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... biotinylated ACE2-hFc or mouse anti-His mAb (R&D systems). Spike trimer (1 µM binding sites ...
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bioRxiv - Cancer Biology 2023Quote: ... The ligands used were His-Tagged human CD38 (R&D systems, catalog # 2404-AC ...
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bioRxiv - Immunology 2021Quote: ... L-SIGN-hFc tag was purchased from R&D systems (USA). Antibody 4A8 was produced in-house according to GenBank accession no ...
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bioRxiv - Biochemistry 2023Quote: ... were from ThermoFisher and histidine-tag antibodies (1:2000) were from R&D Systems. pS255 rabbit polyclonal antibodies were a kind gift from Dr ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: Anti-RBD antibodies were procured to recognise either linear or conformation epitopes (MAB10540 and MAB105802, respectively; R&D Systems). Serum samples were collected either from COVID-19 patients with a positive PCR test in 2020 or prior to 2019 as negative controls ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
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bioRxiv - Cell Biology 2021Quote: ESC to DE transition – 24 hours in S1 medium supplemented with Activin A (100ng/ml, R&D Systems) and CHIR99021 compound (14μg/ml ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with ice-cold acetone and stained with 1:200 dilution of monoclonal-anti-RBD (R&D Systems, MN, USA), polyclonal-anti-S1- ...
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bioRxiv - Bioengineering 2019Quote: Stage 1: 3 days in S1 basal media with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human His-tagged CD80 (R&D Systems 9050-B1, Minneapolis, MN, USA) or CD86 (R&D Systems 9090-B2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant human TIM-4-HIS (R&D Systems, Cat# 9407-TM-050)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were then probed with anti-FLAG tag (1:2000, R&D Systems), anti-pVHL (1:500 ...
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bioRxiv - Immunology 2023Quote: ... and 100 ng/mL anti-HA tag antibody (clone 543851, R&D Systems) to PBMC medium and incubating for 15 min at room temperature to crosslink the proteins ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2.5 µL anti-hEGF tag antibody (Anti-hEGF-biotin, R&D Systems BAF236) was added at a concentration of 0.5 µg per sample and cells incubated at 4 °C for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and bound biotinylated ACE2 and RBD in the presence or absence of SP-A was detected by incubating with Streptavidin-HRP (VANC00B, R&D Systems) for 1 h at room temperature and developed as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...
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bioRxiv - Biophysics 2024Quote: ... Purified kinetochores were incubated with biotinylated α-His antibody (R&D Systems, Minneapolis MN) at an antibody concentration of 0.025 ng/µl for 1 hour at 4 °C with rotation ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems, catalog # IC0501R) or HER2-ED (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies for immunoprecipitation and Western blotting included anti-V5 and anti-His (R&D systems), Mouse anti-VAMP2 (SySy Synaptic Systems ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems, Minneapolis MN) and stored in BRB80 containing 8 mg/ml BSA and 1 mM DTT at 4 °C with continuous rotation ...
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bioRxiv - Cancer Biology 2020Quote: The relative content of 111 cytokine proteins (Table S1) was determined using the dot blot based mouse XL cytokine array kit following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies consisted in the human anti-spike mAb 48 (1:1,000; a gift from H. Mouquet) or the mouse anti-p24 Gag MAB7360 (R&D Systems; 1:1000). Anti-human or mouse IgG secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
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bioRxiv - Neuroscience 2023Quote: ... cells were treated for 24h with 100ng/ml media of human recombinant GBA (rhGBA-His (R&D Systems)).
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bioRxiv - Developmental Biology 2023Quote: ... and a fusion of mPrtg ECD (F36-A952) to a His6 tag (Biotechne R&D Systems 6795-PR) were used as baits ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Gamma (10795-CV-100) and Delta (10878-CV-100) spike proteins were purchased from R&D Systems (R&D Systems, Bio-Techne, MN, USA).
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bioRxiv - Systems Biology 2022Quote: ... Cells were resuspended in an antibody solution containing 5μL DYKDDDDK Epitope Tag Alexa Fluor 647-conjugated Antibody (R&D Systems #IC8529R) and 45μL DBPS + 10% FBS and incubated in the dark at room temperature for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Immunology 2023Quote: ... and 50 U/mL Interleukin-2 (IL-2, R&D Systems). Pre-isolated NK cells were thawed and co-cultured at a 1:1 effector:target (E:T ...
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bioRxiv - Cell Biology 2022Quote: ... Type 2 (BME-2) droplets (R&D Systems, Minneapolis, MN, USA) and covered under medium ...
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bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
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bioRxiv - Biophysics 2022Quote: ... ECD fragments (modified C-terminally with 10xHis tag) Podocalyxin (PODXL) (Cat.# 1658-PD) and PODXL2 (Cat.# 1524-EG) were obtained from R&D Systems. The ECD fragments were dissolved in ultrapure water to a concentration of 0.5 mg/mL and aliquoted in 50 μL ...
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bioRxiv - Biophysics 2024Quote: Recombinant sKLA (Glu34-Ser981) with a C-terminal 6His tag expressed in a NS0 murine myeloma cell line was purchased (R&D Systems) and loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Immunology 2019Quote: ... and macrophage inflammatory protein-2 (MIP-2) (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... DuoSet IC ELISA kit (DYC1767-2 and DYC1766-2, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and 2 ng/ml IL-2 (carrier-free; Tocris R&D Systems). Cells treated with only these functioned as the mock control ...
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bioRxiv - Microbiology 2019Quote: ... and lipocalin-2 (Lcn-2) protein was assayed in Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA). At the end of DSS treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Cell Biology 2020Quote: ... and the mouse chemoattractant macrophage-inflammatory protein-2 (MIP-2, R&D Systems). Migration of cells was evaluated by trypan blue exclusion following incubation.
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bioRxiv - Cancer Biology 2022Quote: ... 2 μg/mL Fibulin-2 (Cat no. 9559-FB-050, R&D Systems) coated ...
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bioRxiv - Neuroscience 2021Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
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bioRxiv - Immunology 2022Quote: ... human IL-2 ELISA (human IL-2 Quantikine ELISA kit, R&D systems, USA) and TNFα ELISA (human TNF-alpha quantikine ELISA kit ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen, Germany; R&D Systems, Minneapolis, USA) and a horseradish peroxidase (HRP)-coupled secondary antibody and CheLuminate-HRP ECL solution (Applichem ...
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... PIGF-2 (R&D systems) and Endoglin/CD105 (R&D systems ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-APB (R&D Systems), SKF96365 (Wako) ...
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bioRxiv - Cancer Biology 2023Quote: ... Type 2 (R&D Systems) and plated on 8-chamber slides (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... Lipocalin-2 (R&D Systems) and high sensitivity TNF (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... and 10 ng/ml fibroblast growth factor-2 (FGF-2, R&D Systems, Minneapolis, MN) (39 ...