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Citations for R&D Systems :
101 - 150 of 157 citations for Oleoyl Gly Lys m PEG11 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained with Human CD11b/Integrin alpha M Alexa Fluor® 405-conjugated Antibody (R&D Systems) at a concentration of 1 µg/106 cells according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 50 ng/mL M-CSF (Cat No. 216-MC, R&D Systems, Minneapolis, Minnesota, United States) at a density of 1 × 10^6 cells/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Sigma Aldrich #A7906) or 50 ng/ml CSF1 (Recombinant Mouse M-CSF, R&D Systems 416-ML-500)(R(0)/CSF1 ...
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bioRxiv - Microbiology 2021Quote: ... with 10% FBS (Wisent) and 0.5 ng/ml recombinant human Macrophage Colony Stimulating factor (M-CSF) (R&D Systems) to allow for differentiation of monocytes into macrophages ...
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bioRxiv - Physiology 2021Quote: ... The medium was supplemented with 100 ng/mL recombinant human M-CSF using CellXVivo™ kit (R&D Systems) and differentiated for 5–7 days prior to use.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...
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bioRxiv - Immunology 2020Quote: ... and 10 ng/mL of human macrophage colony stimulating factor (M-CSF, R&D Systems, catalog number 216-MC) for at least 10 days to generate mature macrophages before use in experiments.
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bioRxiv - Bioengineering 2024Quote: ... 50 ng/mL recombinant human macrophage colony-stimulating factor (#216-MC; M-CSF, R&D Systems, Minneapolis, MN, USA) and 50 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (#215-GM ...
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bioRxiv - Cell Biology 2020Quote: ... and differentiated in complete monocyte derived macrophage (MDM) medium containing 10 ng/ml M-CSF (R&D Systems, Abingdon, UK) for two days ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Microbiology 2021Quote: BMDM were isolated and differentiated in DMEM containing 20% FBS (Cytiva) and 25ng/ml rm-M-CSF (R&D Systems) as previously described [12] ...
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bioRxiv - Cell Biology 2024Quote: ... after cells were induced with 25 ng/mL M-CSF and 25 ng/mL RANKL (R&D Systems, Abingdon, UK). After 9 days of maturation ...
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bioRxiv - Microbiology 2021Quote: ... and CD14+ monocytes were differentiated into macrophages (MDMs) using Macrophage-colony stimulatory factor (M-CSF) (R&D systems, Minneapolis, MN, USA) as previously described [20] ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Cancer Biology 2020Quote: ... Macrophage differentiation from bone marrow cells was obtained by culture in standard growth medium supplemented with m-CSF (R&D Systems) at 30 ng/ml for 7 days.
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bioRxiv - Immunology 2020Quote: ... and RPMI-1640 supplemented as described and with additional 20 mM Hepes and 25 ng/ml M-CSF (R&D Systems). Medium was changed every third day and the cells were allowed to differentiate for 10 days ...
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bioRxiv - Immunology 2021Quote: ... CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well) in the presence of 20 ng/mL M-CSF (216-MC-005; R&D Systems) for 4 days.6 Monocyte-derived macrophages [(Mb) ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... with or without 1.25 µg/mL Scl-Ab or 500 ng/mL anti-M-CSF antibody (#AF416 from R&D Systems). After 8 days ...
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The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... Cells were plated and cultured in complete medium containing 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems). The medium was refreshed every 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Microbiology 2020Quote: ... Fully-differentiated MDM were generated by culturing MC under adherent conditions for seven days using RPMI-AS medium supplemented with 10 ng/mL M-CSF (R&D Systems). T cells were purified from PBMC using T cell pan isolation kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Bioengineering 2020Quote: ... Media for M(IFN-γ) stimulation consisted of RPMI 1640 supplemented with 10 ng/ml interferon-γ (IFN-γ; R&D systems), and media for M(IL4/IL13 ...
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bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cancer Biology 2023Quote: ... Osteoclast differentiation media consisted of standard OCY454 media supplemented with 25ng/ml M-CSF (R&D Systems Catelog # 416-TEC; Lot # ME5021121) and 5 ng/ml RANKL (R&D Systems Catalog #462-TEC-010 ...
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bioRxiv - Molecular Biology 2023Quote: ... over the course of 7 additional days with 25 ng/mL M-CSF and 25 ng/mL RANKL (both from R&D Systems), as previously described [17,30,49] ...
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bioRxiv - Bioengineering 2024Quote: ... then differentiated into M0 macrophage with 25 ng/ml recombinant macrophage colony-stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA) for 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in complete αMEM with 40 ng/ml M-CSF (ProSpec-Tany TechnoGene, Rehovat, Israel) and l0 ng/ml RANKL (R&D Systems, MN). Tartrate-resistant acid phosphatase (TRAcP ...
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bioRxiv - Pathology 2024Quote: ... the BMMs are incubated for three days in a 24-well plate with medium supplemented with 30 ng/ml M-CSF and 50 ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D Systems, MN). After this period ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... resuspended in N2-S medium supplemented with 20 ng/ml EGF and 20 ng/ml FGF2 (both from R&D Systems, Minneapolis, M USA) and seeded as droplets (10 µl ...
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Neuroscience 2021Quote: Differentiated BMDM were plated in 96-well plate at density of 30,000 cells/well either in the presence of 40ng/ml M-CSF (R&D systems, 416-ML-050) or without M-CSF ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in the presence of 50 ng/mL of GM-CSF (02532, Stem Cells, Vancouver, Canada) and 50 ng/mL of M-CSF (216-MC, R&D Systems, Minneapolis, USA) at 37°C and 5% CO2 for differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... non-adherent hematopoietic progenitors were pooled and plated at 7x104 cell/well (96-well) with complete media supplemented with 30 ng/mL M-CSF (R&D Systems, 416-ML). At 90% confluence ...
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bioRxiv - Cancer Biology 2024Quote: Human CCL2/MCP-1 and M-CSF/CSF1 were measured by ELISA using DuoSet kits (DY279, DY216, respectively) from R&D Systems (Minneapolis, USA) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2020Quote: ... Cells were isolated by flow cytometric cell sorting on a BD FACSMelody and cultured in StemPro-34 supplemented with either M-CSF or VEGF (R&D Systems, cat #293-VE).
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bioRxiv - Cell Biology 2022Quote: ... BMMs were then seeded at 1 × 106 cells/well in 24-well plates and stimulated with 30 ng/mL RANKL and 30 ng/mL M-CSF (R&D Systems, Minneapolis, MN, USA) for 5 days to generate mature OCs ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...