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Citations for R&D Systems :
101 - 150 of 3551 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Immunology 2023Quote: ... naïve B cells or CH12F3 cells were treated with 10ng/ml F(ab’)2 Fragment Goat Anti-Mouse IgM or 10ng/ml mIL-5 (R&D Systems) for 5 or 10 min and then collected for Western blotting or RNA extraction.
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Immunology 2022Quote: ... cells were washed and split into two new 48-well plate wells and cultured with IL-2 (5 ng/ml; R&D Systems), in the presence or the absence of CH-223191 (10 µM ...
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bioRxiv - Immunology 2024Quote: Fecal samples collected from female mice at 5 weeks of age were assayed for immunoglobulin and Lcn-2 content using ELISA kits (Mabtech; R&D Systems). Protein was extracted from fecal samples by manual homogenization of 2-3 pellets in PBS + 0.05% Tween20 followed by 4 hr of agitation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and 5 ng/ml IL-5 (R&D Systems) to monitor B cell processes during B cell differentiation or to monitor differentiation with increased plasma cell (PC ...
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bioRxiv - Developmental Biology 2020Quote: ... chick GFP (1:500; 2010, Aves) and goat Contactin-2 (1:100; AF1714 R&D Systems, Minneapolis, MN, USA), Donkey anti-goat-488 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were split 1:3 in SF-D media containing recombinant human (rh) VEGF (5 ng ml-1; R&D Systems), rhBMP4 (10 ng ml-1 ...
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bioRxiv - Systems Biology 2020Quote: ... or mouse anti-TNFα Receptors 1 and 2 (10 µg/mL, R&D Systems) (see Figure 1-Figure Supplement 4B ...
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bioRxiv - Pathology 2021Quote: ... 1% Antibiotic-Antimycotic and 20ng/ml recombinant fibroblast growth factor 2 (R&D Systems)) and passaged using Accutase (Innovative Cell Technologies)19 ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-human angiotensin converting enzyme 2 (ACE2; R&D Systems; 1:500). Appropriate species-specific secondary antibodies conjugated to AlexaFluor555 and/or AlexaFluor647 were applied for fluorescent detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antigen retrieval of ROR (R&D systems, N7927, Lot #A-2, 1:100), coronal 20 μm sections were boiled for 15 minutes in sodium citrate buffer (10mM sodium citrate ...
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bioRxiv - Immunology 2024Quote: ... goat anti-sex determining region Y-box 2 (1:200, R&D Systems, af2018); GFAP ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-SRY-box-containing gene 2 (SOX2, 1:250, AF2018, R&D Systems); rabbit anti-SRY-box-containing gene 2 (SOX2 ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-SRY-box-containing gene 2 (SOX2, 1:250, AF2018, R&D Systems); rabbit anti-SRY-box-containing gene 2 (SOX2 ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... bEFNB2 expressing CHO cells were passaged in puromycin (1-5 ug/mL) for 1 week and then stained with EphB3-Fc (5667-B3-050; R&D Systems), a natural ligand of EFNB2/3 ...
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bioRxiv - Bioengineering 2023Quote: ... we used an N-cadherin Fc chimera (R&D Systems, 1388-NC-050) and a 546-nm fluorescently tagged Protein A (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were incubated at room temperature for 2 hours with primary antibodies (Megalin/LRP2, MyBiosource MBS690201, 1:500) (KIM-1, R&D Systems MAB1817, 1:250) in block solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA) or human IgG isotype control (#31154 ...
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bioRxiv - Microbiology 2024Quote: ... 1×105 spirochetes were pretreated with 0–5 μM hSLPI (R&D Systems, #1274-PI- 100) at 33°C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 ng/ml recombinant human NRG1-β 1 (#396-HB, R&D Systems, USA) as described in Poitelon et al ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... SH-SY5Y and SK-N-AS cells were pre-treated for 16 h with 1000 Units/ml Interferon Type 1 (Interferon α/β, R&D Systems (Minneapolis, MN, USA)) followed by 2,5 μM BV6 (Selleckchem ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 5 days at 4°C with Car4 primary antibody (1:500, RRID:AB_10013483; R&D Systems), rinsed ...
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bioRxiv - Cell Biology 2023Quote: ... Followed by 24 h treatment with 50 nM of Shh-N (R&D Systems) diluted in serum-free DMEM/F-12 ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Pathology 2022Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Systems Biology 2024Quote: ... Intestinal and/or serum levels of LCN2 and IGF-1 were quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA and Mouse/Rat IGF-I/IGF-1 DuoSet ELISA (R&D Systems), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-MAP2 (aka Microtubule-Associated Protein 2; 1:250; R&D Systems, Cat #: MAB8304, RRID:AB_2814693), rabbit anti-Myosin7 (MYO7 ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-rabbit Nucleocapsid (NovusBiologics ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-mouse H3 (Immune-Tech ...
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bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... type 2 (BME-2; R&D Systems; 3532-010-02), and dispensed in a 24-well plate to form 50 µL BME-2 domes for culture ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (DCX, Goat anti-doublecortin, 1:500, Santa Cruz; SOX2, Goat anti-sex determining region Y-box 2, 1:200, R&D Systems; CLR ...
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bioRxiv - Bioengineering 2022Quote: ... Microvessels with and without 24 h treatment with 5 ng mL-1 human recombinant TNFα (R&D Systems), were perfused with THP1s under low shear stress (∼0.2 dyne cm-2 ...