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Citations for R&D Systems :
101 - 150 of 1936 citations for Junctional adhesion molecule B JAM B Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... all the wells were treated with TGFb1 (R&D systems, #240-B-010, 5ng/ml). Drugs were used at a concentration of 10µM ...
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bioRxiv - Cancer Biology 2019Quote: ... Active CD73 enzyme (Recombinant Human 5’-Nucleotidase/CD73 Protein, CF) and active CD39 (His-Tag Human) were from R&D Systems, Minneapolis ...
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bioRxiv - Bioengineering 2020Quote: ... Mouse IL-28 A/B (IFN-lambda 2/3) Du-oSet ELISA (DY1789B, R&D Systems), was used to assess cross-reactivity.
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bioRxiv - Developmental Biology 2019Quote: ... or DMEM+10% FBS+10 ng/mL TGFβ1 (Cat. # 240-B, R&D Systems, Minneapolis, MN). A P200 tip was used scratch down the midline of every well ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with or without 10 ng/ml TGFβ (R&D Systems, Cat. # 240-B) for another 24 hr and harvested for RNA extraction after 72 hr.
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bioRxiv - Bioengineering 2023Quote: ... B cell activating medium was supplemented with 5 ug/mL anti-CD40 antibody (R&D systems) and 20 ng/mL IL-4 (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... starved overnight and exposed to 1.5 ng/ml of TFGβ (#100-B-001, R&D Systems) for 6 and 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... stimulation with transforming growth factor β (TGF-β, 240-B, R&D Systems, Minneapolis, MN, USA), dissolved in 0.1% (weight/volume [w/v] ...
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...
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bioRxiv - Microbiology 2020Quote: ... stimulated B cells were incubated with 20μg/ml anti–DC-SIGN mAb (clone 120507, R&D system) or mouse IgG (R & D Systems ...
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bioRxiv - Genetics 2023Quote: ... and receptor activator of nuclear factor kappa-B ligand (RANKL) (2 ng/mL; R&D systems; USA) to induce osteoclastogenesis ...
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bioRxiv - Cell Biology 2023Quote: ... TGF-β pathway was stimulated by adding rhTGF-β1 ligand (2ng/mL; R&D Systems; 240-B) for 24 hours ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... The medium was replaced with fresh differentiation medium containing 10 ng/mL VEGF-B (R&D Systems) every two days ...
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Cell Biology 2020Quote: ... to RAW264.7 or 2 ng/mL transforming growth factor β-1(240-B/CF, R&D System, USA) to THP-1 for 24-36 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... granzyme B and IFN-γ secretion were measured using ELISA as described by the manufacturer (R&D Systems).
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bioRxiv - Neuroscience 2023Quote: Molecules used: EGF (R&D Systems, 236-EG), FGF2 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Systems Biology 2020Quote: ... culture medium was replaced with fresh medium supplemented with 4.0 ng/ml TGF-β (R&D Systems 240-B) for 1-3 days to induce EMT ...
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bioRxiv - Immunology 2020Quote: Primary murine B cells were incubated in 20 mM β-lactose or 0.1 μM recombinant Gal9 (R&D systems) in RPMI1640 at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatants of these samples were subjected to Granzyme B secretion analysis using an ELISA kit (R&D Systems). Following detection of the activated cell populations in certain samples ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated for 24h with 100ng/ml media of human recombinant GBA (rhGBA-His (R&D Systems)).
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro ubiquitylation assay reactions in 1 x ubiquitin conjugation reaction buffer (R&D systems Cat. # B-70) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro SUMOylation assay reaction in 1 x SUMO conjugation reaction buffer (R&D systems Cat. # B-60) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Immunology 2022Quote: ... recombinant SARS-CoV-2 Spike RBD and recombinant SARS-CoV-2 Spike RBD B.1.617.2 L452R T478K were purchased from R&D systems. Proteins were reconstituted as per the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared to obtain solutions of TGF-β1 (10 ng/mL and 25 ng/mL, R&D Systems 240-B-002), palmitic acid (PA ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... in Medium B with the addition of 5 U/mL erythropoietin (EPO; Amgen) and 5 ng/mL interleukin-3 (IL3; R&D Systems). Two days later ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... B-27 Serum-Free Supplement (50x) (Cat. No. 17504044, Life Technology) + 50 ng/ml FGF7 (Cat. No. 251-KG, R&D System) for 2 days ...
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bioRxiv - Neuroscience 2022Quote: ... We used 250 µg of cell lysates for each of the parts A and B of the array and followed manufacturers’ instructions (R&D Systems).
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bioRxiv - Microbiology 2023Quote: ... and the medium was removed and washed with 1× PBS before viral infection and TGF-β1 (catalog #240-B-002, R&D Systems) treatment ...
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bioRxiv - Immunology 2023Quote: ... cells were initially activated at 5 x 105 cells/mL in B cell activation media: 5 µg/mL soluble CD40L (R&D Systems), 10 µg/mL anti-His tag antibody (clone AD1.1.10 ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Immunology 2022Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
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bioRxiv - Microbiology 2021Quote: ... the BMMs became adherent for the osteoclastic differentiation using a complete DMEM supplemented with 50 ng/ml of receptor activator of nuclear factor kappa-B ligand (RANKL, R&D System, USA) and 20 ng/ml of M-CSF (R&D System ...
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bioRxiv - Biochemistry 2021Quote: Acvr1[R206H]/+and Acvr2a −/−;Acvr2b −/−;Bmpr2 −/−;Acvr1[R206H]/+ mES cells were dissociated using non-enzymatic cell dissociation buffer (MiliPore, S-004-B) and resuspended in the flow cytometry staining buffer (R&D systems, FC001). After 15 minutes of blocking (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Pathology 2022Quote: ... was seeded with 10 000 myoblasts in serum-free medium while the wells were filled with either proliferating medium or serum-free medium complemented with 10 ng.mL-1 FGF-b (R&D Systems 3139-FB), IGF-I (R&D Systems 791-MG ...
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bioRxiv - Cancer Biology 2023Quote: IFN-γ and granzyme B concentrations in the supernatants of CD8+ T cells were measured using a mouse ELISA kit (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Biochemistry 2021Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...