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Citations for R&D Systems :
101 - 150 of 231 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... granzyme B and IFN-γ secretion were measured using ELISA as described by the manufacturer (R&D Systems).
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bioRxiv - Cancer Biology 2024Quote: ... ENAS + 5 ng/ml TGF-β1 (Recombinant Human TGF-β1 Protein, R&D Systems, Cat. No. 240-B). Basal medium + solvent ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were stimulated with 2 ng/mL recombinant human TGF-β1 (R&D Systems, cat. # 240-B) diluted in starvation medium for varying durations of 30 ...
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bioRxiv - Cell Biology 2024Quote: ... LVEC were treated with vehicle or TGF-β1 (10 ng/ml, R&D Systems, Cat# 240-B-010) for 7 days as described before5 unless otherwise indicated ...
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bioRxiv - Cell Biology 2024Quote: ... EV-splenocyte coculture supernatants were assayed for Mouse Granzyme-B DuoSet ELISA (R&D Systems, Cat#DY1865-05) and IFNγ ELISAs antibody (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of stop solution (Sulfuric acid) (R&D systems) was added ...
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bioRxiv - Cell Biology 2022Quote: ... A stock solution of recombinant mouse Wnt3A (R&D systems) was prepared at 40 μg/ml in 0.1% BSA/PBS and used between 10-100 ng/ml concentration in culture.
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bioRxiv - Neuroscience 2022Quote: ... Pre warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and the absorbance was monitored at 450 nm with a Fluorostar galaxy plate reader.
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bioRxiv - Neuroscience 2022Quote: ... Pre-warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and absorbance at 450 nm was measured in a Fluorostar galaxy plate reader.
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bioRxiv - Microbiology 2022Quote: ... The assay was developed with substrate solution (R&D systems) and the reaction was stopped with 1M H2SO4 and analyzed with a Tecan Sunrise microplate reader at (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... and 2.5% trypsin-EDTA solution (R&D systems, Cat. B81710) were added to the tubes and incubated for 30 min at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... by incubation in Cultrex Organoid Harvesting Solution (R&D Systems) for 1 hour on ice and mechanical dissociation with a pipette ...
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bioRxiv - Molecular Biology 2024Quote: ... solidified and immersed in prechilled lysis solution (R&D systems) overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: Organoids were isolated using organoid harvesting solution (R&D Systems). Dissolved CultrexTM BME was aspirated ...
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bioRxiv - Bioengineering 2020Quote: ... 97 µL of urine was incubated with 1 µL of human cathepsin B (R&D Systems, 953-CY-010) and 1 µL of DTT for 15 minutes to inactivate the urine cysteine protease inhibitor ...
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bioRxiv - Systems Biology 2020Quote: ... culture medium was replaced with fresh medium supplemented with 4.0 ng/ml TGF-β (R&D Systems 240-B) for 1-3 days to induce EMT ...
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bioRxiv - Immunology 2020Quote: Primary murine B cells were incubated in 20 mM β-lactose or 0.1 μM recombinant Gal9 (R&D systems) in RPMI1640 at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatants of these samples were subjected to Granzyme B secretion analysis using an ELISA kit (R&D Systems). Following detection of the activated cell populations in certain samples ...
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bioRxiv - Neuroscience 2024Quote: ... Abcam Cat# ab25630, RRID:AB_470708 1:1000, GCase Sigma-Aldrich Cat# G4171, RRID:AB_1078958 1:1000, Cathepsin B R&D Systems Cat# AF965 ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... recombinant SARS-CoV-2 Spike RBD and recombinant SARS-CoV-2 Spike RBD B.1.617.2 L452R T478K were purchased from R&D systems. Proteins were reconstituted as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... and developed for 10 min using substrate solution (R&D systems). The reaction was stopped with 2N H2SO4 and plates were measured using a microplate reader (Tecan Sunrise ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
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PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistancebioRxiv - Cancer Biology 2022Quote: ... epithelial to mesenchymal transition (EMT) was induced with 200 pM of Human recombinant TGF-beta 1 (R&D Systems, 240-B); verteporfin (Sigma Aldrich ...
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Bioengineering 2023Quote: ... Fibroblasts were treated with or without recombinant human transforming growth factor beta 1 (TGF-β1) protein (240-B, R&D Systems) at 1.25 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: ... IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems) (53 ...
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bioRxiv - Immunology 2024Quote: ... 5×104 labeled cells were resuspended in B cell media (RPMI, 5%FCS, 50μM 2-mercaptoethanol and 2mM glutamine) and stimulated with 50ng/ml CD40L (R&D Systems), 50ng/ml IL-4 (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2024Quote: Primary fibroblast cultures were used unstimulated (NF) or activated by exposure to 10 ng/ml TGFβ (TGFβ1; 240-B-002/CF; R&D Systems) for 72 hours (AF) ...
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bioRxiv - Biochemistry 2021Quote: Four 4× stock solutions were prepared containing (i) Ube1 (R&D Systems) + UbcH5c/UBE2D3 (R&D Systems ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10% BSA ELISA reagent diluent/blocking solution concentrate (DY995, R&D systems) and TMB substrate (421501 ...
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bioRxiv - Immunology 2024Quote: ... or of Cultrex Organoid Digestion solution (R&D systems, #3700-100-01). Chips were incubated for 45 minutes at 4°C to allow diffusion of the digestion solution from the upper to the lower channel ...
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bioRxiv - Systems Biology 2024Quote: ... cells were stained with 6 µM Calcein AM solution (R&D Systems) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were lysed overnight in Comet Assay Lysis Solution (R&D Systems). The next day ...
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bioRxiv - Bioengineering 2021Quote: ... and 100 U/mL Penicillin/Streptomycin supplemented with 0.1% recombinant human Fetal Growth Factor b (Alamone F-170) and 0.1% recombinant human Epidermal Growth Factor (R&D systems 236-EG). On Day 12 ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Genomics 2021Quote: ... Isolated B cells were cultured in IMDM medium supplemented with 10% FBS and 55 mM beta-mercaptoethanol at 1X106 cell/mL and activated by CellXVivo Human B cell expander (1:250 dilution, R&D system) and 50 ng/mL IL2 (Cat#200-02-10ug ...
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bioRxiv - Cell Biology 2020Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... B-27 Serum-Free Supplement (50x) (Cat. No. 17504044, Life Technology) + 50 ng/ml FGF7 (Cat. No. 251-KG, R&D System) for 2 days ...
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bioRxiv - Neuroscience 2022Quote: ... We used 250 µg of cell lysates for each of the parts A and B of the array and followed manufacturers’ instructions (R&D Systems).
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bioRxiv - Microbiology 2023Quote: ... and the medium was removed and washed with 1× PBS before viral infection and TGF-β1 (catalog #240-B-002, R&D Systems) treatment ...
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bioRxiv - Immunology 2023Quote: ... cells were initially activated at 5 x 105 cells/mL in B cell activation media: 5 µg/mL soluble CD40L (R&D Systems), 10 µg/mL anti-His tag antibody (clone AD1.1.10 ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... and IgG in the culture supernatants from stimulated B cells were determined by ELISA kits for human MPO (R&D Systems, #DY3174), mouse MPO (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... TGF-β1 was reconstituted in sterile 4 mM HCl + 0.1% BSA according to manufacturer’s instructions (R&D Systems Cat #240-B-002). Indicated conditions were treated with the TGF-β1 inhibitor SB 431542 (Sigma-Aldrich Cat# S4317 ...
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bioRxiv - Bioengineering 2021Quote: ... Cerebrum primary antibody solution contained mouse anti-GFAP IgG (R&D Systems, MAB2594) and DAPI to visualize astrocytes and cell nucleus respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... 3-day old organoids were harvested with Cultrex Harvesting Solution (R&D Systems) and frozen in Recovery Cell Culture Freezing Medium (GIBCO) ...