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Citations for R&D Systems :
101 - 150 of 2547 citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: Collagen fibrils were formed by combining rat tail tendon-derived acid-soluble type I collagen (5 mg/mL in 20 mM acetic acid) (CultrexRat Collagen I, R&D Systems) with 400 mM HEPES + 0.02% sodium azide and 1 M NaCl at room temperature to reach final concentrations of 1 mg/mL collagen ...
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bioRxiv - Cancer Biology 2024Quote: ... one million cells were suspended in 100 µl of 1:1 RPMI and BME-Type 3 (R&D Systems, # 36-320-1002P) per implantation ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in N2B27 medium supplemented with 12 ng/ml bFGF (R&D Systems, cat. 233-FB-025/CF), 20 ng/ml Activin A (PeproTech ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were serum-starved for 12 h and then treated with 100 ng/ml BMP2 (R&D Systems) for 3 h ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL of IL-2 and 10 ng/mL of IL-12 (R&D systems, 419-ML-010)) ...
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bioRxiv - Immunology 2023Quote: ... Purified cells from five subsets were cultured in IL-12 (20 ng/ml, 219-IL-005; R&D Systems), IL-18 (20 ng/ml 9124-IL-010 ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... enzyme linked immunosorbent assay (ELISA) kit for IL-12/IL-23 (p40) was obtained from R&D Systems (Minnesota, USA); ELISA kit for IL-6 ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasma galectin-3 from male and female wild-type and heterozygous mice was measured using a DuoSet ELISA Development System kit (D1197; R&D Systems, Minneapolis, MN). Wells were coated with 100 μL of a 2 μg/mL dilution of the galectin-3 capture antibody and only 1 μL of plasma was required per well ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blotted using the primary antibodies: Ebi3 (2.5 μg/ml; eBioscience) or IL-12/IL-35 p35 (1 μg/ml; R&D Systems), phospho-mTOR (Ser2448 ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Cell Biology 2024Quote: ... medium was changed to experimental medium (Ham’s F-12 supplemented with 4% HS and 1X penicillin/streptomycin [R&D Systems; B21210]) for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were converted to an epiblast-like (EpiLC) state by 48 hour culture in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Microbiology 2021Quote: ... IL-1β (cat No. DY401) and IL-12 p70 (cat No. DY419) by using the mouse DuoSet ELISA system (R&D Systems). In addition ...
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bioRxiv - Microbiology 2022Quote: Cover glass (FisherBrand, 12-546-P) was sterilized in 70% EtOH and coated with BME Cultrex (R&D Systems, 3432-010-01) prior to seeding ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Microbiology 2023Quote: ... The culture supernatants were analyzed for cytokines using the Human TNF-α and Human IL-12 DuoSet ELISA kits (R&D Systems) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: Crypts isolated from mouse intestine were seeded at a density of 250 crypts per 25 µL Matrigel (Cultrex reduced growth factor basement membrane extract, type R1, R&D Systems, Catalog #3433-010-R1) in 48 well plates ...
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bioRxiv - Immunology 2023Quote: ... anti-CD86 and anti-CD206) except for anti-TF antibody (AF3178; R&D Systems). Cell viability was measured using the LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (ThermoFisher Scientific) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... anti-CCL20 and anti-IFNγ (R&D Systems) by using Cytofix/CytoPerm Plus kit (BD Pharmingen ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested after treatment and stained for PD-L1 (eBioscience, 12-5983-42) and MHC class I (R&D Systems, FAB7098G). Induction of the respective proteins was analyzed by flow cytometry.
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were seeded in tissue culture-treated 12-well plates coated with human fibronectin (5 µg/cm2) (R&D Systems, Cat. # 1918-FN). Stimulated and unstimulated groups were formed as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... SH-SY5Y and SK-N-AS cells were pre-treated for 16 h with 1000 Units/ml Interferon Type 1 (Interferon α/β, R&D Systems (Minneapolis, MN, USA)) followed by 2,5 μM BV6 (Selleckchem ...
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bioRxiv - Immunology 2024Quote: ... anti-CXCL10 or anti-CXCL11 mAb (R&D systems) were added to the lower compartment prior to start of the assay.
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bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections from formalin-fixed paraffin-wax embedded tumours were stained with primary rat anti-mouse monoclonal anti-CD8 antibody (eBioscience, 4Sm15as) and goat anti-mouse polyclonal anti-PD-L1 antibody (R&D systems, AF1019). Slides were scanned and imaged using Hamamatsu Nanozoomer (Hamamatsu Photonics).
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bioRxiv - Bioengineering 2024Quote: ... were transferred into Neural Induction Media (NIM) composed of DMEM/F-12 supplemented with 1x N-2 MAX Media Supplement (R&D Systems, Cat no. AR009), 1x GlutaMAX ...
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bioRxiv - Immunology 2023Quote: ... anti-VEGF and anti-TGF-β detection antibodies (R&D Systems) diluted in BSA 1% (1:1000) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-CD8 (AbD Serotec) goat anti-human LTA (R&D systems); rabbit anti-laminin (Wako ...
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bioRxiv - Immunology 2020Quote: ... Anti-TNFR1 (16803) and anti-TNFR2 (22210) were from R&D Systems. Ruxolitinib was from Seleckchem ...
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bioRxiv - Immunology 2020Quote: ... IL-15 and IL-18 (rhIL-12, R&D Systems, 5 ng/mL; rhIL-15, Pepro-Tech, 20 ng/mL; rhIL-18, R&D Systems, 50 ng/mL), or left unstimulated without further intervention ...
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bioRxiv - Pathology 2021Quote: ... mouse anti-TGF-β and goat anti-Serpin F1/PEDF (R&D Systems), rabbit anti-Uteroglobin ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-EPCAM (AF960) and Anti-AMPN (AF3815) antibodies were from R&D Systems. Antibodies against β-actin (sc-47778) ...
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bioRxiv - Immunology 2022Quote: ... goat polyclonal anti-myeloperoxidase (anti-MPO, R&D Systems, cat. AF3667, 1:100) and rabbit polyclonal ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2.5 µL anti-hEGF tag antibody (Anti-hEGF-biotin, R&D Systems BAF236) was added at a concentration of 0.5 µg per sample and cells incubated at 4 °C for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD74 (sheep anti-mouse cd74, polyclonal antibody, Cat# AF7478, R&D system), anti-PU1 (clone 9G7 ...
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bioRxiv - Immunology 2024Quote: ... goat anti human/mouse myeloperoxidase (anti-MPO, AF3667, R&D Systems, Minneapolis, MN), rabbit anti human/mouse citrullinated histone 3 (H3Cit ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293; anti-PTDGS/PGD2 R&D Systems CAT#MAB10099 1:100 ...