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Citations for R&D Systems :
101 - 150 of 622 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... mouse TNF-alpha DuoSet ELISA (DY410) and DuoSet ELISA Ancillary Reagent Kit 2 (DY008) were purchased from R&D system.
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bioRxiv - Immunology 2024Quote: ... ELISA kits were used to quantified ferritin (Christal Chem) and soluble mouse CD25/IL-2 alpha receptor (R&D Systems) following manufactorers instructuon.
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bioRxiv - Cell Biology 2024Quote: ... and human collagen I alpha 1 were assessed utilizing magnetic Luminex® Assays (R&D systems; #LXSARM-03 / #LXSAHM-04). The secretome samples used for these assays consisted of three samples from each culturing group that were pooled (n = 3) ...
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bioRxiv - Cell Biology 2020Quote: ... Once the cells were adherent the cells undergoing differentiation were cultured in basal medium supplemented with 1% v/v Adipogenic Supplement (R&D Systems) while the control cells not undergoing differentiation were cultured in basal medium ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with Annexin V (R&D Systems) per manufacturer’s instructions and were run immediately without fixation or were fixed in 1% PFA diluted in Annexin V binding buffer ...
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bioRxiv - Immunology 2020Quote: ... IL-5 (5 ng/ml, R&D Systems), TGF-β (3 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA ...
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bioRxiv - Cell Biology 2021Quote: ... which was constructed using the cDNA sequences of mouse IL-6/IL-6R alpha fusion protein (9038-SR, R&D systems). AAV8-Null vector was used as vector control.
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: HEK293 cells and HEK293 stably transfected with membrane bound KL (HEK293-mKL cells) were cultured in Alpha MEM (SH30265.01; Hyclone, Marlborough, MA) supplemented with 10% fetal bovine serum (S11195; R&D Systems), 1% L-Glutamine 100X Solution (Hyclone) ...
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bioRxiv - Immunology 2024Quote: ELISA kits were used to quantified serum ferritin (Christal Chem) and soluble mouse CD25/IL-2 alpha receptor (R&D Systems) following manufacturers instruction.
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bioRxiv - Immunology 2021Quote: ... and 5 ng/ml IL-5 (R&D Systems) to monitor B cell processes during B cell differentiation or to monitor differentiation with increased plasma cell (PC ...
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bioRxiv - Genetics 2021Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Immunology 2022Quote: ... Urine albumin and creatinine were measured using ELISA kits (Alpha Diagnostic International, San Antonio, USA, and R&D systems, Minneapolis, USA, respectively). Urine samples were diluted to fit within the standards range of each kit ...
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bioRxiv - Neuroscience 2022Quote: ... 506-RL-010/CF) and carrier free Recombinant Mouse TNF-alpha (aa 80-235) Protein (410-MT-025/CF) purchased from R&D Systems.
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Microbiology 2023Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants were transferred to a new eppendorf tube and used for measuring the levels of cytokines using the Mouse TNF-alpha Quantikine ELISA kit and the Mouse IL-6 Quantikine ELISA kit (R&D systems), following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: CCL3 and MPO concentrations were detected in immune cell supernatants after 24 h stimulation using Mouse CCL3/MIP-1 alpha DuoSet ELISA and Mouse Myeloperoxidase DuoSet ELISA kits (both R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Bioengineering 2023Quote: VeroE6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (ThermoScientific, Waltham, MA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Bio-Techne, R&D systems, Minneapolis, MN, USA) and 1% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... Culture supernatants were collected after the second treatment and TNF-α levels were quantified by ELISA using the Human TNF-alpha DuoSet ELISA (R&D systems) following manufacturer recommendations.
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies and dilutions for human muscle cross section imaging: anti-human PDGFR alpha antibody 1:100 (R&D Systems, #AF-307-NA), anti-Laminin 2 alpha 1:250 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Concentrations of IFN-α in culture supernatants or mouse serum were measured using Mouse IFN-alpha All Subtype Quantikine ELISA Kit (MFNAS0, R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Biochemistry 2020Quote: ... cathepsin V (1080-CY) and were purchased from R&D Systems. Human cathepsin K (BML-SE553 ...
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Bioengineering 2020Quote: ... After the STOP solution (R&D system), the plates were read at multiple wavelengths (the optical density at 550 nm wavelength subtracted from the density at 450 nm ...
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bioRxiv - Bioengineering 2020Quote: ... After the STOP solution (R&D system), the plates were read at multiple wavelengths (the optical density at 550 nm wavelength subtracted from the density at 450 nm ...
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bioRxiv - Immunology 2022Quote: ... After the STOP solution (R&D system), the plates were read at multiple wavelengths (450 nm and 550 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 M/mL PyMT tumor cells were suspended in collagen +/-L-ribose supplemented with basement membrane extract [20 % v/v final (R&D Systems, Cultrex BME, 3532-005-02) and DMEM [1x final] and the pH was adjusted to ∼7.3 ...
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bioRxiv - Bioengineering 2020Quote: ... Following two washes, the samples were incubated in 200 μl of substrate solution (tetramethylbenzidine, TMB solution) (R&D systems) per sample for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
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bioRxiv - Bioengineering 2023Quote: RFP-MDA-MD-231 and GFP-HUVEC at 3 million/mL and 8 million/mL in precursor solution were photopatterned and perfused using the peristaltic pump previously mentioned at 0.2 rpm/mL for five days before tumor necrosis factor-alpha (TNF-α) (R&D Systems, Minneapolis, MN, USA) and Vascular endothelial growth factor (VEGF ...
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bioRxiv - Immunology 2020Quote: ... developed using TMB solution (R&D Systems, #DY999) and read at 450nm.
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Immunology 2022Quote: ... single allele APCs were resuspended in CRPMI (w/o phenol red) +10% fetal bovine serum (FBS, R&D Systems) containing 1 µg/mL of each peptide and incubated overnight ...
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bioRxiv - Cell Biology 2021Quote: ... (v) 100 ng/ml Act A + 10 ng/ml FGF4 (R&D Systems) or (vi ...
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bioRxiv - Immunology 2021Quote: ... Cells were assessed for apoptosis with Annexin V staining kit (R&D Systems) per manufacturer’s instructions and were analyzed immediately via flow cytometry.
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bioRxiv - Microbiology 2022Quote: ... TACS® Annexin V-FITC Apoptosis Detection Kit (R&D Systems cat# 483001K) was used and cells were stained according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Annexin V/propidium iodide apoptosis detection kits were obtained from R&D Systems Inc ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8A hispid cotton rat (Sigmodon hispidus) sequences were taken from commercially available constructs (Cotton Rat CD4 VersaClone cDNA, cat# RDC1063, R&D Systems; Cotton Rat CD8 alpha (AAL55392) VersaClone cDNA ...