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101 - 150 of 1044 citations for 4 2 Trimethylsilyl Ethyl Aniline since 2020

• ATM and MSH2 control blunt DNA end joining in immunoglobulin class switch recombination

  Emily Sible, et al., bioRxiv - Immunology 2022

  Quote: ... and 12.5 ng/ml IL-4 (R&D Systems). B cells were split 1:2 v/v 48 and 72 hours after isolation ...
• Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end joining reactions independently of 53BP1-governed repair pathway choice

  Ashleigh King, et al., bioRxiv - Molecular Biology 2023

  Quote: ... mouse IL-4 (5 ng/μl; R&D Systems) and TGFβ1 (2.5ng/μl ...
• A Serum- and Feeder-Free System to Generate CD4 and Regulatory T Cells from Human iPSCs

  Helen Fong, et al., bioRxiv - Cell Biology 2024

  Quote: ... SSEA-4 (#MAB1435) were obtained from R&D Systems. TRA-1–81 (#MAB8495 ...
• Epithelial heme oxygenase-1 enhances colonic tumorigenesis by inhibiting ferroptosis

  Rosemary C. Callahan, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 4-HNE antibody was purchased from R&D Systems. Representative photomicrographs were obtained using an EVOS m5000 microscope.
• A “ tug-of-war” between the NuRD and SWI/SNF chromatin remodelers regulates the coordinated activation of Epithelial-Mesenchymal Transition and inflammation in oral cancer

  Roberto Stabile, et al., bioRxiv - Cancer Biology 2024

  Quote: ... with IL-4 (20 ng/mL, R&D Systems), or with IL-10 (20 ng/mL R&D Systems) ...
• An AlphaFold2 map of the 53BP1 pathway identifies a direct SHLD3-RIF1 interaction critical for DNA repair activity

  Chérine Sifri, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and 10 ng/mL mIL-4 (R&D Systems). After 48 h ...
• Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype

  Rebecca L. Schmitz, et al., bioRxiv - Bioengineering 2023

  Quote: ... and 20 ng/mL IL-4 (R&D Systems) (6 ...
• MYC directly transactivates CR2/CD21, the receptor of the Epstein-Barr virus, and enhances the viral infection of Burkitt lymphoma cells

  Ester Molina, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and IL-4 (20 ng/mL; R&D Systems) for 48 hours ...
• Neuro-Regenerative Behavior of Adipose-Derived Stem Cells in Aligned Collagen I Hydrogels

  Mackenzie Lewis, et al., bioRxiv - Bioengineering 2023

  Quote: ... Custom-designed 4-Plex Luminex kits (R&D Systems) were used to quantify beta-nerve growth factor (βNGF) ...
• The Implant-Induced Foreign Body Response is Limited by CD13-Dependent Regulation of Ubiquitination of Fusogenic Proteins

  Mallika Ghosh, et al., bioRxiv - Cell Biology 2023

  Quote: ... Fusion inducing cytokines IL-4 (R&D Systems; 404ML010) and IL-13 (R&D Systems ...
• BRWD1 establishes epigenetic states for germinal center initiation, maintenance, and function

  Nathaniel E. Wright, et al., bioRxiv - Immunology 2024

  Quote: ... and 10 ng/ml IL-4 (R&D Systems) were added to the media.
• GPR43 in eosinophils prevents the emergence of pathogenic Siglec-F^hi neutrophils in allergic airway inflammation

  Jihyun Yu, et al., bioRxiv - Immunology 2024

  Quote: ... and 10 ng/ml IL-4 (R&D Systems), respectively ...
• Identification of a Neuroimmune Circuit that Regulates Allergic Inflammation in the Esophagus

  Kendall Kellerman, et al., bioRxiv - Immunology 2024

  Quote: Murine recombinant IL-4,(Peprotech and R&D Systems), capsaicin ...
• Stress-induced Rab11a-exosomes induce AREG-mediated cetuximab resistance in colorectal cancer

  John D. Mason, et al., bioRxiv - Cancer Biology 2023

  Quote: AREG-neutralising experiments were performed by pre-incubating freshly prepared EVs (at 4 x 103 EVs per recipient cell) with either 4 µg/ml of goat anti-AREG (R&D Systems #AF262) or goat anti-IgG (R&D Systems #AB-1080-C ...
• T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells

  Ritika Tewari, et al., bioRxiv - Cell Biology 2020

  Quote: ... IL-2 concentration was measured using a human IL-2 ELISA kit (R&D Systems, DY202-05) following the manufacturer’s instructions.
• A tissue-engineered human trabecular meshwork hydrogel for advanced glaucoma disease modeling

  Haiyan Li, et al., bioRxiv - Bioengineering 2020

  Quote: ... 2) transforming growth factor beta-2 (TGF-β2; 2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), 3 ...
• Endothelial Rbpj is essential for the education of tumour-associated macrophages

  Ronja Mülfarth, et al., bioRxiv - Cancer Biology 2022

  Quote: ... inserts were coated for 2 hours with 2 μg/ml fibronectin (1918-FN-02M; R&D Systems) in PBS ...
• Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress

  Maria Naama, et al., bioRxiv - Immunology 2022

  Quote: ... Fecal Lcn-2 levels were determined by the Lcn-2 ELISA kit (R&D systems, DY1857-05) according to the manufacturer’s instructions ...
• SIRPα sequesters SHP-2 to promote IL-4/13 signaling and alternative activation of macrophages

  Lei Shi, et al., bioRxiv - Immunology 2021

  Quote: ... in which Lcn-2 levels were assayed using a murine Lcn-2 ELISA kit (R&D Systems). For macrophage adoptive transfer ...
• Intestinal epithelial MHC class II regulation by HDAC3 instructs microbiota-specific CD4+ T cells

  Emily M. Eshleman, et al., bioRxiv - Immunology 2022

  Quote: ... Supernatants were collected and lipocalin-2 levels were determined using mouse Lipocalin-2/NGAL (R&D Systems) following the manufacturer’s instructions.
• Development of an optimized, non-stem cell line for intranasal delivery of therapeutic cargo to the central nervous system

  Ali El-Ayoubi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... the following antibodies were used: anti-MMP-2 (AF902, 2 μg/ml; R&D Systems, Abington, UK); anti-MMP-9 (MAB-911 ...
• Rescue of secretion of a rare-disease associated mis-folded mutant glycoprotein in UGGT1 knock-out mammalian cells

  Gábor Tax, et al., bioRxiv - Cell Biology 2023

  Quote: ... An anti Trop-2 mAb (T16) and and anti Trop-2 pAb (AF650 from R&D Systems) were used to perform staining of live ...
• PD-1 negatively regulates helper T cell differentiation into Th2

  Masaki Tajima, et al., bioRxiv - Immunology 2024

  Quote: ... and IL-2 levels were determined using mouse IL-2 DuoSet ELISA (R&D Systems, catalog# DY402).
• The novel ECM protein SNED1 mediates cell adhesion via the RGD-binding integrins α5β1 and αvβ3

  Dharma Pally, Nandini Kapoor, Alexandra Naba, bioRxiv - Cell Biology 2024

  Quote: ... and supplemented with 25 ng/mL fibroblast growth factor-2 (FGF-2, R&D systems, #233-FB). All cell lines were maintained at 37°C in a 5% CO2 humidified incubator.
• Sinking the way: a dual role for CCR7 in collective leukocyte migration

  Jonna Alanko, et al., bioRxiv - Cell Biology 2022

  Quote: ... for 2 days in R10 medium supplemented with IL-2 (10ng/ml, 402-ML-020, R&D Systems) for 2 days ...
• Pathobionts from chemically disrupted gut microbiota induce insulin-dependent diabetes in mice

  Xin Yang, et al., bioRxiv - Microbiology 2022

  Quote: Fecal lipocalin-2 content was quantified with a Lipocalin-2/NGAL Quantikine ELISA kit (DLCN20, R&D Systems).
• Ym1 protein crystals promote type 2 immunity

  Ines Heyndrickx, et al., bioRxiv - Immunology 2023

  Quote: ... CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems). Serum Ym1-specific immunoglobulin M (IgM ...
• The degree of T cell stemness differentially impacts the potency of adoptive cancer immunotherapy in a Lef-1 and Tcf-1 dependent manner

  Guillermo O. Rangel Rivera, et al., bioRxiv - Immunology 2023

  Quote: ... IL-2 complex was made with 1.5μg of recombinant human IL-2 (NIH) and 7.5μg of anti-human IL-2 (R&D Systems) per injection in 100μL of sterile PBS ...
• Fecal Microbiota Restoration Modulates the Microbiome in Inflammation-Driven Colorectal Cancer

  Travis J Gates, et al., bioRxiv - Microbiology 2022

  Quote: ... Lcn-2 levels were approximated using a Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
• Aquaporins enriched in endothelial vacuole membrane regulate the diameters of microvasculature in hyperglycemia

  Changsheng Chen, et al., bioRxiv - Cell Biology 2023

  Quote: ... EBs were formed in an ESC medium depleted of recombinant human FGF-2 (rhFGF-2) (R&D Systems) on day 0 ...
• Identification of a novel enhancer essential for Satb1 expression in T_H2 cells and activated ILC2s

  Aneela Nomura, et al., bioRxiv - Immunology 2023

  Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
• mRNA Vaccine-Induced SARS-CoV-2 Spike-Specific IFN-γ and IL-2 T-cell Responses are Predictive of Serological Neutralization and are Transiently Enhanced by Pre-Existing Cross-Reactive Immunity

  Philip Samaan, et al., bioRxiv - Immunology 2024

  Quote: ... or CEFTA were supplemented with 10IU/mL of recombinant human IL-2 (rh IL-2; R&D Systems) and 10ng/mL of recombinant human IL-7 (rh IL-7 ...
• Clostridioides difficile binary toxin CDT induces biofilm-like persisting microcolonies

  Jazmin Meza Torres, et al., bioRxiv - Microbiology 2024

  Quote: ... Lcn-2 levels were estimated in the supernatants using Duoset murine Lcn-2 ELISA kit (R&D Systems). Samples from day 0 (before infection ...
• Altered temporal sequence of transcriptional regulators in the generation of human cerebellar granule cells

  Hourinaz Behesti, et al., bioRxiv - Developmental Biology 2021

  Quote: ... BMP4 (range tested: 4-160 ng/ml, R&D Systems), 100 ng/ml GDF7 (R&D Systems) ...
• A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

  Joep Beumer, et al., bioRxiv - Molecular Biology 2021

  Quote: ... goat anti-DPP-4 (1:200; R&D systems, AF1180) and rabbit anti-MERS S1 (1:200 ...
• Nuclear stiffness decreases with disruption of the extracellular matrix in living tissues

  Kaitlin P. McCreery, et al., bioRxiv - Biophysics 2020

  Quote: Recombinant human ADAMTS-4 (R&D Systems, Minneapolis, MN, USA) and MMP-13 (R&D Systems ...
• ZEB1 is Required for NHEJ-Mediated DSB Repair in Euchromatin

  Thomas L. Genetta, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 5 ng/ml recombinant murine IL-4 (R&D Systems), and 5 ng/ml recombinant human TGF-β (R&D Systems) ...
• Identifying novel B-cell targets for chronic inflammatory autoimmune disease by screening of chemical probes in a patient-derived cell assay

  Yvonne Sundström, et al., bioRxiv - Immunology 2020

  Quote: ... rhIL-4 (final concentration 200 ng/ml, R&D Systems) and CpG ODN2006 (final concentration 1 μg/ml ...
• Thrombospondin-4 mediates hyperglycemia- and TGF-beta-induced inflammation in breast cancer

  Santoshi Muppala, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and goat anti-human TSP-4 (AF2390, R&D Systems) using Vecta Stain ABC Kit (PK-6104 ...
• Thrombospondin-4 mediates hyperglycemia- and TGF-beta-induced inflammation in breast cancer

  Santoshi Muppala, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and goat anti-human TSP-4 (AF2390, R&D Systems). Sections of human tumors were stained with mouse anti-human CD68 (Dako ...
• Anti-GD2 antibody disrupts GD2:Siglec-7 interactions and synergizes with CD47 blockade to mediate tumor eradication

  Johanna Theruvath, et al., bioRxiv - Immunology 2021

  Quote: ... 4 μg/mL of each Siglec-Fc (R&D Systems) was pre-complexed with either 8 μg/mL AffiniPure Donkey anti-human IgG Alexa Fluor 647 (Jackson ImmunoResearch ...
• Generation of CD34⁺CD43⁺ hematopoietic progenitors to induce thymocytes from human pluripotent stem cells

  Léa Flippe, et al., bioRxiv - Developmental Biology 2021

  Quote: ... All recombinant factors were purchased from PeproTech with the exception of hBMP-4 from R&D Systems.
• Cystatin F (Cst7) drives sex-dependent changes in microglia in an amyloid-driven model of Alzheimer’s Disease

  Michael J. D. Daniels, et al., bioRxiv - Neuroscience 2022

  Quote: ... IL-4 (20 ng/mL, 24 h, R&D Systems) or apoptotic SH-SY5Y neuroblastoma cells (2:1 ratio neurons:microglia ...
• Human pluripotent stem cells-derived inner ear organoids recapitulate otic development in vitro

  Daniela Doda, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and BMP-4 (314-BP-010 Biotechne/R&D Systems) from 0.5-10ng/ml ...
• PD-1 negatively regulates helper T cell differentiation into Th2

  Masaki Tajima, et al., bioRxiv - Immunology 2024

  Quote: ... Mouse IL-4 DuoSet ELISA (R&D Systems, catalog# DY404) and Mouse IL-13 DuoSet ELISA (R&D Systems ...
• Integrating central and peripheral neurons in elongating multi-lineage-organized gastruloids

  Zachary T. Olmsted, Janet L. Paluh, bioRxiv - Developmental Biology 2020

  Quote: ... 2 ng/ml HGF (R&D Systems) and 50 μM Y-27632 (Tocris Bioscience) ...
• A Quantitative Model of Cellular Decision Making in Direct Neuronal Reprogramming

  Adriaan Merlevede, et al., bioRxiv - Systems Biology 2020

  Quote: ... GDNF (2 ng/mL, R&D Systems), NT3 (10 ng/µL ...
• Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate

  Endika Prieto-Fernández, et al., bioRxiv - Cell Biology 2021

  Quote: ... syndecan-2 (R&D Systems Cat#: FAB2965P), syndecan-3 (R&D Systems Cat# ...
• A Combined Human Gastruloid Model of Cardiogenesis and Neurogenesis

  Zachary T. Olmsted, Janet L. Paluh, bioRxiv - Developmental Biology 2022

  Quote: ... 2 ng/ml HGF (R&D Systems) and 50 μM Y-27632 (Tocris Bioscience) ...
• Splice switching oligonucleotide mediated gene knockdown in B cells and plasma cells

  Anne Marchalot, et al., bioRxiv - Immunology 2020

  Quote: ... 5ng/mL hIL-2 (R&D Systems) and 100ng/mL Mega-CD40 ligand (Enzo Life Sciences ...