Labshake search
Citations for R&D Systems :
1401 - 1450 of 1942 citations for SPINK4 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... was titered using a commercial enzyme-linked immunosorbent assay kit (Human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml−1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells surface ACE2 protein was stained with Alex Flour® 647-conjugated human ACE2 antibody (R&D Systems FAB933R). Live/Dead (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The level of human or mouse CXCL10/IP-10 was determined using a DuoSet ELISA kit (R&D Systems) and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were exposed to the Human/Mouse Brachyury Affinity Purified Polyclonal Goat IgG antibody (R&D Systems, Catalog # AF2085) overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell-free supernatant was collected and quantified using the Human IL-12p70 DuoSet ELISA (R&D Systems; catalog# DY1270), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...
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bioRxiv - Immunology 2020Quote: ... and 10 ng/mL of human macrophage colony stimulating factor (M-CSF, R&D Systems, catalog number 216-MC) for at least 10 days to generate mature macrophages before use in experiments.
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bioRxiv - Immunology 2021Quote: ... The concentration of sCD25 was measured with the Human CD25/IL-2R alpha Quantikine ELISA kit (R&D Systems) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The binding of pig and rabbit (Thymoglobulin®, Sanofi) IgG to human FcγRIIIa (CD16a, R&D Systems, 4325-FC) receptor was also measured by SPR ...
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bioRxiv - Cell Biology 2021Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... secreted matrix metalloproteinases (MMP) concentrations were measured using the magnetic Human MMP Magnetic Luminex Performance Assay (R&D Systems). This assay required dilutions of cell culture supernatants in fresh culture medium at either 2x or 200x to detect the reported analytes within a quantifiable range.
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bioRxiv - Molecular Biology 2022Quote: ... Day 0: 10 ng/ml recombinant human FGF basic/FGF2/bFGF (146 aa) protein (R&D Systems 233-FB), 10 µM RI (what is RI?) ...
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bioRxiv - Bioengineering 2022Quote: ... Enzyme-Linked Immunosorbent Assay (ELISA) kit (DuoSet ELISA Ancillary Reagent Kit and Human Lubricin/PRG4 kit, R&D systems) was used following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cell supernatants were then harvested and assayed for cytokines by Human Cytokine Array (Proteome Profiler, R&D systems, ARY005B), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL recombinant human glial cell line-derived neurotrophic factor (GDNF, #212-GD-010, R&D Systems, Germany), 500 µM cAMP (#D0260 ...
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bioRxiv - Physiology 2023Quote: ... Diluted serum was then used to measure human HB-EGF with the DuoSet ELISA Kit (DY259B, R&D Systems).
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bioRxiv - Microbiology 2022Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and analyzed for the presence of secreted factors using Luminex human Discovery Assays (R&D Systems): LXSAHM-02 (MMP-8) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... each well was then filled with 25 μL/well recombinant human TNFα (R&D systems, cat #: 210-TA-100) diluted in cell culture medium (final concentration 10 ng/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The Human Aβ1-42 in soluble and insoluble fractions were qualified with Quantikine ™ ELISA kit (R&D Systems) based on the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... in absence or presence of stimuli (PMA/ionomycin or human recombinant soluble BAFF/BLyS/TNFSF13B Protein (R&D System)) for 18 h at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cytokine array measurements were made using the Proteome Profiler Human XL Cytokine Array Kit (ARY022B) from R&D systems, following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The differentiated organoids were treated with the indicated doses of recombinant human IL-1β (R&D system, 201-LB) for an additional 48 hrs.
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Biophysics 2023Quote: ... and then a binding buffer solution containing 200 nM of recombinant αVβ5 human integrin extracellular domains (R&D Systems) was slowly added to one of the wells of the fluid chamber ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Human vasculature labeling was performed by using a cocktail of goat anti-podocalyxin (1:20, AF1658, R&D Systems), mouse anti-CD31 (1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... and IL6 releases were analysed by Luminex® Assay using the Human Premixed Multi-Analyte Kit (R&D Systems) according to manufacturer instructions and acquiring readings with a BioPlex MagPlex Magneti Beads system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with mouse anti-human CCR1 antibody (2.5 µg/106 cells; mab145, R&D Systems) for 20 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: MSCs were stimulated with recombinant human IFNα (0.03 U/ml) or IFNβ (60 pg/ml) protein (R&D Systems), in combination with different concentrations of inhibitor of IFNα/β (i-IFNα/β ...
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bioRxiv - Neuroscience 2023Quote: ... and B2M in collected media were examined using Human Magnetic Luminex® Assays (R&D Systems; Cat. # LXSAHM-03) according to the manufacturer’s instructions and read on a MAGPIX instrument (Luminex Corp).
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Physiology 2024Quote: ... PDGFR-α (goat anti human, 1:300, cat. no.: AF-307-NA; RRID: AB_354459, R&D Systems, Bio-Techne), and C3 (rabbit anti human ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the conditioned media were assayed with Proteome Profiler Human XL Cytokine Arrays (ARY022B; R&D Systems, Minneapolis, MN, USA) performed as per manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA, R&D Systems) and a decrease of ≥0.3 OD450 value between post and pre-surgery sera was considered significant ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 50 ng/mL human bone morphogenetic protein 4 (hBMP4) (R&D Systems, Minneapolis, Minnesota, USA), 50 ng/mL human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Microbiology 2024Quote: ... The relative levels of cytokines were assessed using the Proteome Profiler Human XLCytokine Array kit (R&D Systems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were suspended in 200 μL of a 125 nM solution of recombinant human SIRPα-Fc protein (R&D Systems), the cognate isotype control recombinant human IgG1 protein (Sino Biological) ...
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bioRxiv - Developmental Biology 2021Quote: ... the medium was switched to FE-BM1 or BM2 containing 20 ng/ml recombinant human BMP4 (rhBMP4, R&D Systems), 50 nM retinoic acid (RA ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial suspensions were incubated with human SP-D at a concentration of 10mg/ml (R&D Systems, 1920-SP-050) for 1h at RT without agitation and observed by light microscopy (Nikon Ti Eclipse equipped with a metal-oxide-semiconductor (sCMOS ...
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bioRxiv - Developmental Biology 2021Quote: ... coli-derived human N-terminal modified (C24II) fragment of Sonic Hedgehog (Shh-N; R&D Systems, Cat#1845-SH/CF) was reconstituted in PBS at 10µM and stored at -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was collected and processed using the Proteome Profiler Human Cytokine Array Kit (R&D Systems; Cat. No. ARY005B), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20). To reduce the skewness of phenotype distributions ...
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bioRxiv - Neuroscience 2019Quote: Human TNFα was quantified in blood serum (1:10 dilution) using a DuoSet ELISA kit (R&D Systems, Minneapolis, MN). Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution ...
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bioRxiv - Immunology 2019Quote: ... following relevant treatment with or without 200 U/ml of recombinant human (h) or mouse (m) TNFα (R&D Systems). Protein concentration was measured using the Bradford assay (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Biophysics 2019Quote: ... Ibidi channels IV0.4 (Clinisciences) were coated overnight at 4°C with 10μg/ml human ICAM-l-Fc (R&D Systems) in Phosphate Buffer Solution (PBS ...
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bioRxiv - Immunology 2021Quote: ... IL-11 was quantified by using a Human IL-11 Quantikine ELISA Kit according to manufacturer instructions (R&D Systems). Statistical analysis was performed on log10-transformed values with two-way ANOVA tests and Tukey correction ...