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Citations for R&D Systems :
1401 - 1450 of 1504 citations for Pyruvate Kinase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cytokine levels of TNFα was quantified in serum obtained from mice infected with and without Salmonella for 24 h by the mouse/rat TNF-A Valukine ELISA kit (R&D Systems, #VAL609) following the product protocol.
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bioRxiv - Microbiology 2023Quote: ... Supernatants were used to quantify the amount of cyto/chemokines produced by the urothelial organoids before and after infection using a human premixed multi-analyte customized kit in a Luminex® bead based immunoassay (R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: IFN-γ and granzyme B concentrations in the supernatants of CD8+ T cells were measured using a mouse ELISA kit (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: All mouse serum samples were analyzed by a custom R&D systems Murine Premixed Multi- Analyte Kit (R&D Systems, Inc., Minneapolis, MN). The panel was run on a Luminex 200 Instrument (Luminex Corporation ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Immunology 2023Quote: Levels of cytokines and chemokines were assessed in serum samples by sandwich ELISA using commercial kits (DuoSet R&D Systems, Minneapolis, MN, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... All sections were incubated with a biotinylated secondary antibody and stained using the R&D HRP-DAB staining kit (R&D Systems, USA) and then counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of epidermal growth factor (EGF) protein levels in the serum was performed with the Mouse EGF Quantikine ELISA Kit (MEG00, R&D Systems Inc.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The supernatant of BALF was store at -80°C for cytokine analysis by mouse TNF-α and IL-1β DuoSet ELISA kits (R&D Systems). Lungs were perfused with a 1:1 mixture of OCT and 4% PFA and preserved in OCT cryomolds (Tissue-Tek ...
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bioRxiv - Immunology 2023Quote: ITCH ubiquitination reactions were carried out using the manufacturer’s protocol for the Human ITCH/AIP4 Ubiquitin Ligase Kit (R&D Systems, K-270). 10X reaction buffer ...
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bioRxiv - Physiology 2024Quote: ... USA) and blood FGF21 levels were assayed by a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Neuroscience 2024Quote: Secreted proteins in iTF-Microglia conditioned media were measured using the Proteome Profiler Human Cytokine Array Kit (R&D Systems; Cat. No. ARY005B) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Supernatants (1 mL from individual wells in 24-well plates and 250 μL from individual wells in 96-well plates) were collected and directly used for ELISAs (DuoSet® ELISA kits from R&D Systems). Supernatants from LPS conditions were diluted 1:5 for IL-10 quantification and 1:10 for TNF-□ quantification to avoid reaching the upper limit of detection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: TNF-α and IL-6 in BAL fluids and serum samples were measured using ELISA kit (TNF-α: MTA00B; IL-6: M6000B-1) from R&D Systems, according to the recommended protocols from the manufacturer.
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bioRxiv - Immunology 2024Quote: ... Concentrations of IFN-α in culture supernatants or mouse serum were measured using Mouse IFN-alpha All Subtype Quantikine ELISA Kit (MFNAS0, R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2020Quote: Mouse CXCL1/KC and interleukin-6 concentrations in lung homogenates from infected mice were determined using DuoSet® ELISA kits (R&D Systems, UK) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: Adiponectin levels in the gonadal white adipose tissue was determined using Quantikine mouse adiponectin/Acrp 30 ELISA kit from R&D systems (MN, USA). Liver homogenates were measured by a solid-phase ELISA technique according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: FGF-21 levels in the serum and liver were determined using Quantikine mouse/rat FGF-21 ELISA kit from R&D systems (MN, USA). Serum and liver homogenates were measured by a solid-phase ELISA technique according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasma galectin-3 from male and female wild-type and heterozygous mice was measured using a DuoSet ELISA Development System kit (D1197; R&D Systems, Minneapolis, MN). Wells were coated with 100 μL of a 2 μg/mL dilution of the galectin-3 capture antibody and only 1 μL of plasma was required per well ...
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bioRxiv - Cell Biology 2020Quote: ... IL-6 and TNFα cytokine levels in the media were then measured with DuoSet ELISA kits (R&D systems, catalog number DY406 and DY410) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2019Quote: ... secreted IL-2 samples were diluted 1:10 and subsequently analyzed using Mouse IL-2 Quantikine ELISA Kit (R&D Systems Inc, Minneapolis, MN), as per manufacturer’s specifications.
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bioRxiv - Neuroscience 2021Quote: ... The relative expression profile of 36 human cytokines and chemokines were evaluated using the Proteome Profiler Human Cytokine Array Kit (R&D Systems, ON, Canada). The experiment was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The samples were diluted 1:5 using diluent buffer provided by kit and analyzed by sandwich ELISA for human CCL3 and human CCL4 according to manufacturer’s instructions (R&D Systems, DMB00 and DMA00).
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell lines were purchased from ATCC (Manassas, VA, USA) and confirmed to be mycoplasma free by MycoProbe kit (R&D Systems, Minneapolis, MN, USA). Cells were grown at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Interleukin (IL)-6 in the patient plasma samples was measured by the Quantikine ELISA kit for human IL-6 (R&D Systems, Minneapolis, MN), as previously [29] ...
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bioRxiv - Microbiology 2020Quote: ... fourth weeks of infection were collected to evaluate the concentration of MCP-1 and TNF-α by using ELISA kits according to the manufacturer’s protocols (R&D Systems, Rennes, France), the mean minimum detectable dose of human MCP-1 was 1.7 pg/mL and 4.00 pg/mL for human TNF-α ...
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bioRxiv - Immunology 2022Quote: ... the blood was centrifuged with 2000g for 20min and the supernatant (serum) was collected for TPO measurement using Quantikine Mouse Thrombopoietin ELISA Kit (R&D Systems, Minneapolis, USA) to measure serum TPO levels ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Immunology 2022Quote: ... The release of cytokines IL-1β and TNF-α into supernatants was determined with ELISA kits (R&D Systems, DY401-05 and DY410-05) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein was applied to each ELISA membrane and developed according to the manufacturer’s instructions (Proteome Profiler Mouse XL Cytokine Kit, ARY028, R&D Systems, Minneapolis, MN, USA). Cytokine array signals were detected by X-ray film ...
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bioRxiv - Molecular Biology 2019Quote: ... administered intravenously to BALB/C mice at a dose of (0.05mg/kg of body weight and measured for serum hEpo concentration using Human Erythropoietin Quantikine IVD ELISA kit (R&D Systems cat. no. DEP00) at 6 hours post injection.
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bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
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bioRxiv - Physiology 2021Quote: ... HOMA-IR was calculated as (fasting glucose × fasting insulin)/22.5 and HOMA-B as (20 × fasting insulin)/(fasting glucose – 3.5).(31) GDF15 concentration was measured with Human GDF-15 Quantikine Elisa Kit (R&D Systems, Minneapolis, MN, USA). Serum GDF15 samples during and after pregnancy were diluted 1:64 and 1:4 ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were genetically authenticated by the Australian Cancer Research Foundation (ACRF) Cancer Genomics Facility (Adelaide, Australia) and determined mycoplasma-free using the MycoProbe mycoplasma detection kit (CUL001B, R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2021Quote: Concentration of Activin A in human and mouse serum samples were performed using sandwich ELISA-Activin A immunoassay kit from R&D system (Cat DAC008).
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bioRxiv - Immunology 2021Quote: Multiplexed ELISA on interstitial fluid from matched T and NAT fresh tissues were performed on a Luminex 200 platform (Luminex Inc.,) using custom kits of pre-mixed antibody-coated beads (R&D System Inc., MN), which included the following analytes ...
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bioRxiv - Cancer Biology 2020Quote: The relative content of 111 cytokine proteins (Table S1) was determined using the dot blot based mouse XL cytokine array kit following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 were assayed using a Luminex mouse pre-mixed Multi-Analyte kit according to standard instructions (LXSARM-06, R&D System, Minnesota, USA).
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was used to measure levels of inflammation marker Lipocalin-2 using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Neuroscience 2024Quote: ... BDNF standards and tissue protein levels were run in triplicate per manufacturer instructions using Total BDNF Quantikine ELISA Kit (Catalog #DBNT00, R&D Systems, Minneapolis, MN), a sandwich enzyme immunoassay technique ...
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bioRxiv - Cancer Biology 2023Quote: ... chemokines and acute phase proteins were simultaneously assessed using the proteome profiler human XL cytokine array kit [ARY005B] from R&D Systems (Abingdon, UK). Cell lysates were obtained from A375 following treatment with 0.1 MOI of RT3D and 0.1 mM talazoparib at 48 hours post-treatment ...
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bioRxiv - Immunology 2024Quote: BAL from IAV non-infected / infected mice were assayed for cytokine levels using commercially available sandwich ELISA kits for IFN-β and IFN-α(R&D Systems). All experiments for cytokine analysis by ELISA were performed in biological triplicates ...
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bioRxiv - Molecular Biology 2024Quote: ... CCL2 and TL1A were measured using human CCL2 (MCP-1) and human TL1A ELISA kits by R&D Systems (R&D Systems, Minneapolis, MN). SEMA3C was measured using the human SEMA3C ELISA kit by LS Bio (LS Bio ...
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bioRxiv - Molecular Biology 2024Quote: IL-1β and IL-10 amounts were measured in the cultured media of treated cells by commercially available ELISA kit as described by manufacture (Quantikine, R&D Systems, Abingdon, UK). Data were normalized to LPS and presented as percentage change in cytokines release ± SEM.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The measurement of VEGF from mouse endometriosis lesions was performed according to manufacturer’s instructions using mouse VEGF quantikine ELISA Kit (Cat# MMV00, R&D systems, Minneapolis, MN, USA).
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bioRxiv - Evolutionary Biology 2023Quote: Lipocalin-2 concentration in the supernatants was measured using the commercial kit Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) for mouse Lipocalin-2 (DY1857) ...
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bioRxiv - Bioengineering 2024Quote: The effective concentration of IL-15 in the samples was assessed using the Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA). All subsequent steps after sample collections were carried out following the instructions provided by the kit ...
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bioRxiv - Physiology 2022Quote: ... Plasma ICAM-1 and VCAM-1 were measured using the Mouse ICAM-1/CD54 and VCAM-1/CD106 Quantikine ELISA kits according to the handbooks (R&D Systems, Minneapolis, USA).