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Citations for R&D Systems :
1401 - 1450 of 4325 citations for Levonorgestrel LNG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: The p24 ELISA with supernatant from infected and non-infected LPMC cultures was performed according to the manufacturer’s protocol (R&D Systems, Minneapolis, USA). Absorbance was measured using the Spark® 10M multimode microplate reader (Tecan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in BALF were quantified by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol (DY406, DY410 and DY401 from R&D systems; Minnesota, USA). The UV absorbance at 450 nm and 570 nm were measured for the primary signal and for plate correction ...
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bioRxiv - Physiology 2021Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and Ccl19/Ccl21 levels were determined by indirect sandwich of enzyme-linked immunosorbent assay (ELISA) following manufacturer’s instructions (R&D Systems, Minneapolis, MN). Multi-analytic profiling beads using Milliplex® Multiplex mouse cytokine/chemokine panel I kit (EMD Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... The concentration of hCG-β in the culture medium was measured using the Human CG beta (HCG beta) Duoset ELISA (#DY9034-05, R&D Systems) according to the manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems).
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bioRxiv - Pharmacology and Toxicology 2023Quote: Levels of vascular endothelial growth factor (VEGF) were evaluated by sandwich enzyme-linked immunosorbent assay (ELISA) in GL261 cell supernatants using matched antibody pairs from R&D Systems (USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Doses were previously optimized and mice were intravenously injected with 5×1010 genome copies of both Control- and TSLP-AAV for 14 days with serum TSLP levels confirmed using a murine specific ELISA (R&D Systems MTLP00)4.
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 was measured by ELISA (enzyme-linked immunosorbent assay) with the obtained supernatant and analyzed according to the manufacturer’s protocol (R&D Systems, Minnesota, USA).
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bioRxiv - Cell Biology 2023Quote: ... plasma and tissues of interest were collected at different post-infection times and assessed by ELISA using commercially available antibodies and according to the procedures supplied by the manufacturer (R&D Systems, Minneapolis). All samples were stored at -20°C until processing ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected at indicated times and protein concentrations were determined by ELISA following the manufacturer’s instructions for IL-6 (R&D systems, DY406-05), TNFα (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and HRP-streptavidin 1000 (R&D Systems) diluted and were added in each well ...
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bioRxiv - Neuroscience 2019Quote: CXCL12 concentration in supernatants of CXCL12 Ch/DS NC was quantified using Mouse CXCL12 DuoSet ELISA as per supplier instructions (R&D systems, Minneapolis, USA). Encapsulation efficiency and drug loading were calculated as follow:
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bioRxiv - Cancer Biology 2022Quote: The levels of murine TRAIL/TNFSF10 (DY1121) in the cells were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (R&D Systems, Emoriville, California, USA). The OD value was measured by Microplate Reader (Multiskan Spectrum ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
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bioRxiv - Pathology 2020Quote: ... The culture media was then harvested and the concentrations of human IL-1β and IL10 were measured using ELISAs (DY201 and DY217B, R&D Systems, Oakville, ON).
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bioRxiv - Immunology 2020Quote: Secreted IL-1b and MCP-1 proteins in supernatants of monocytic cells stimulated with TNF-α were quantified using sandwich ELISA following the manufacturer’s instructions (R&D systems, Minneapolis, USA).
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bioRxiv - Immunology 2019Quote: ... and the tissue levels of myeloperoxidase (MPO) in the supernatants were determined using a mouse MPO ELISA (R&D systems, Biotechne, Minneapolis, MN, USA). The fixed tissues (left lungs ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Neuroscience 2022Quote: ... we determined the amount of HB-EGF per cell through enzyme linked immunosorbent assay (ELISA; Cat# DY8239-05, R&D Systems, Minneapolis, MN, USA). For analysis of HB-EGF levels in brain tissue ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and WNT16 levels were determined in senescent fibroblast lysates using a standardized sandwich Enzyme-Linked Immunoassay (ELISA) technique (R&D Systems, Minneapolis, MN, USA), according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Cytokine secretion was carried out as a validation from the results of the human cytokine array using a range of human ELISAS from R&D Systems (see below).
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested to measure the release of IFN-γ using a IFN-γ-specific ELISA according to the manufacturer’s instructions (R&D Systems, DY28B05). To evaluate T cell activation markers ...
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Bioengineering 2023Quote: ... IP-10/CXCL10 release from mouse cells was assayed using the Quantikine Mouse IP-10 ELISA assay (R&D Systems, Catalog No. DY466-05) using cell-free supernatants of stimulated cells according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants or sulcus fluid from patients with different disease stage were evaluated for MMP-9 content using ELISA Duo Set (DY911, R&D System, Wiesbaden, Germany.) following the manufactureŕs protocol.
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bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... 50 µL of murine plasma was then used to quantify glucagon concentrations with ELISA by following the manufacturer’s protocol (R&D Systems, cat no. DGCG0). Final glucagon concentrations were calculated based on a standard curve.
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bioRxiv - Molecular Biology 2024Quote: ... The secretion of IFN-γ was also quantified in cell supernatants using a sandwich mouse IFN-γ Duo Set ELISA (DY485-05, R&D systems). Standards and samples were diluted appropriately to obtain results within the stated detection levels.
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bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2019Quote: ... 5.0 × 105 PCs and FBs were grown in a single well of a 24-well dish for 24 hours and enzyme-linked immunosorbent assay (ELISA) was performed per manufacture protocol (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Pathology 2020Quote: ... TNF-α and IL-1β) in the frozen distal small intestine and colon were measured by porcine specific ELISA assays (R&D Systems, Abingdon, UK).
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bioRxiv - Physiology 2019Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their co-incubation with T2 cells pulsed with MART-126–35 (commercially synthesized and purified [>95%] by reverse-phase HPLC by Biomer Technology, Cheshire, UK) by ELISA (R&D Systems, Minneapolis, MN, USA). The MART-126–35–-reactive clones were further expanded in a second-round of exposure to 30 ng/ml anti-CD3 ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant samples (1 ml) and a Proteome Profiler Mouse XL Cytokine Array Kit (#ARY028, R&D Systems) were used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cell culture plates were coated with recombinant DLL4-FC (R&D systems) overnight at 4°C at 0.5 μg/mL prior to the addition of FLS and cytokines ...
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bioRxiv - Pathology 2021Quote: ... and grown on Cultrex coated plates (R&D Systems, 3434-010-02). Cells were routinely passaged using our automated platform in the presence of Accutase (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... The TNF-α concentration in the supernatant was quantified using the Duoset® enzyme-linked immunosorbent assay (ELISA) development system (R&D Systems, Abingdon, Oxfordshire, UK). Three wells for each CSF were used to estimate variation in the experiments ...