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Citations for R&D Systems :
1351 - 1400 of 2603 citations for Recombinant Mouse Ctla4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction.
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bioRxiv - Neuroscience 2020Quote: ... L-glutamine and 10 ng/ml of carrier-free murine recombinant granulocyte and macrophage colony-stimulating factor (GM-CSF, 415-ML, R&D systems, Minneapolis, MN). Cell suspension from one brain was plated in a T75-cell culture flask coated with poly-D-lysine (P7280 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Cancer Biology 2022Quote: ... miniloci in an endogenous IgH and Igκ knockout background (19,20) were immunized with recombinant human LAG-3–Fc protein (hLAG-3–hFc) (R&D Systems, Minneapolis, MN, USA), consisting of the extracellular domain of LAG-3 (Leu23-Leu450 ...
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bioRxiv - Cancer Biology 2022Quote: ... +/-1 µg/mL anti-CD28 (CD28.2) antibody (eBioscience/Thermo 16-0289-81) or recombinant human CD58-Fc chimera protein (R&D Systems 10068-CD-050) at 37 ºC for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: PBMC (2×106 per well) were stimulated in 24-well plates with peptide (individual/pool) plus recombinant IL-2 (R&D Systems Europe Ltd.) at a final concentration of 5μg/mL and 20IU/mL ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Bioengineering 2020Quote: ... medium was removed and the scaffolds designated for study in normal growth media were placed in wells with fresh normal growth medium and the mineralized collagen-AM scaffolds designated for study in inflammatory conditions were placed in wells with inflammatory medium (1 ng/ml of IL-1β recombinant porcine protein (R&D Systems, Minneapolis, MN) added to normal growth media) ...
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bioRxiv - Genetics 2020Quote: ... culture medium was replaced with KSR medium (Chambers et al., 2009, 2011) supplemented with 200 ng/ml of recombinant human Noggin (R&D Systems, Minneapolis, USA) and 10 μM of SB431542 (Sigma-Aldrich) ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Human MΦ were then polarized to a M1 activation state with 30 ng/mL human recombinant IFN-γ (R&D Systems, 285-IF-100) and LPS (100 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Microbiology 2022Quote: WT mice were intraperitoneally (IP)-injected every other day with 500 ng of recombinant IL-25 (R&D Systems; Cat# 7909-IL-010/CF) or equivalent volume of sterile PBS (vehicle control ...
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bioRxiv - Physiology 2022Quote: ... and were further stratified to either treatment of 5 d/wk injections of recombinant sclerostin (0.1 mg/kg body mass) (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline control ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2024Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate were cultured in DMEM base medium supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1% v/v FBS with or without 100 ng/ml Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN). Medium change was performed at day 3 and images were taken at 3 and 5 days using a Keyence BZ-X800 Fluorescence Microscope (Keyence ...
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Bioengineering 2024Quote: Dye-labeled antimicrobial therapeutics (VHHα-(ABD)2-(EEG)6-Sx-POL-Cy7 or VHH16-ABD-(EEG)6-S17-PNT4-sulfo-Cy7) were incubated with recombinant human ADAM10 (R&D Systems, MN, U.S.A.) at 10 μM and 250 nM final concentrations respectively ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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bioRxiv - Immunology 2023Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Immunology 2024Quote: PBMC (2×106 per well) were stimulated in 24-well plates with peptide (individual/pool) plus recombinant IL-2 (R&D Systems Europe Ltd.) at a final concentration of 5µg/mL and 20IU/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Fc-conjugates for mouse EphB2 (467-B2-200) and mouse ephrinB1 (473-EB-200) were obtained from R&D Systems. Following the incubation period ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG2a mAb to CD45 (GAP 8.3) was from ATCC and mouse anti-CD98 mAb was from R&D Systems. Rabbit antibodies (Ab ...
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bioRxiv - Biochemistry 2022Quote: VEGF levels in mouse retinas were determined using the Quantikine mouse VEGF ELISA kit (R&D Systems, Minneapolis, MN, USA) as previously described (Jeon et al. ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... 200μg of total mouse protein samples were analyzed respectively with a Mouse XL Cytokine Array Kit (R&D Systems; ARY028) per manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse IL-3 (403-ML) and 10 ng/mL mouse L-6 (406-ML) (R&D Systems). Total RNA was extracted using the RNeasy Mini kit (QIAGEN ...
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bioRxiv - Developmental Biology 2020Quote: ... or mouse anti-human CD9 (R&D Systems) for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Spp1 (Mouse, R&D Systems AF808, 1:50). Primary antibody incubations were done overnight at 4C except for Chat ...
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bioRxiv - Immunology 2021Quote: ... or mouse (1:2000, R&D Systems, HAF018) secondary antibody ...
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bioRxiv - Developmental Biology 2019Quote: ... goat anti-mouse LYVE1 (R&D Systems, AF2125), rabbit anti-mouse KI-67 (Neomarkers ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse anti NR2E3 (1:100, R&D Systems) and Goat anti IBA1 (1:500 Abcam) ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (1:100, R&D Systems, mouse, MAB2419), insulin (1:12000 ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse EGF (10ng/ml, R&D Systems 2028EG200), and Y-27632 compound (10 µM ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse IL6 (406-ML, R&D Systems). This FGF23 peptide contains an arginine to glutamine amino acid substitution at position 179 which yields it resistant to furin protease-mediated degradation ...
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bioRxiv - Cancer Biology 2020Quote: Mouse anti-human monoclonal antibodies (R&D systems) were used to neutralize CXCL8/IL-8 (IgG1 Clone # 6217) ...
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bioRxiv - Cell Biology 2021Quote: ... 25 ng/mL mouse Wnt3a (R&D Systems).
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... mouse anti-GAPDH (1:1,000, R&D System). For secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-SOX2 (1:50, R&D Systems), guinea pig anti-GLAST (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-TRAIL-R2 (MAB6311, R&D Systems), mouse IgG1 κ isotype control (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-TRAIL-R1 (MAB347, R&D Systems), mouse anti-TRAIL-R2 (MAB6311 ...