Labshake search
Citations for R&D Systems :
1301 - 1350 of 4069 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... were determined by the CXCL2/MIP-2 DuoSet enzyme linked immunosorbent assay (ELISA) kit to the manufacturer’s instructions (R&D Systems). Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
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bioRxiv - Biochemistry 2022Quote: ... cells were treated for 2 hours prior to infection with the IC50 concentration of anti-ACE2 antibody (R&D Systems: AF933) determined for the cell lines used ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).
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bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
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bioRxiv - Microbiology 2024Quote: ... Isolated naive CD4+ T cells were resuspended in isolation media supplemented with recombinant IL-2 (R&D Systems, #202-IL-050) at 10 ng/mL (growth media ...
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Neuroscience 2020Quote: ... in DMEM/F-12 and plated into either 60 mm or 100 mm tissue culture dishes as described above with the addition of rmIL-1α or rmIL-1β (50 or 500 pg/mL, R&D Systems, Minneapolis, MN)) for treatment groups ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: The hiPSCs were cultured in mTesRTM1 (STEMCELL 85850) on 8.68 ug/cm2 (or 0.5 mg per 6 well-plate) Cultrex reduced growth factor (RGF) (R&D Systems 3433-005-01) coated 6-well plates ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Cell Biology 2020Quote: Relative phosphorylation levels of 43 kinases and 2 related proteins were assessed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Slit2 concentrations in mice serum were determined by the sandwich ELISA method with the DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... six- to eight-week old NSG mice were subcutaneously injected into the flank with 2×106 wildtype or pre-transduced A-673 or RD-ES EwS cells in Cultrex Basement Membrane Extract (R&D Systems) to enhance tumor formation ...
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bioRxiv - Microbiology 2022Quote: ... then stained with 0.1 µg/sample Alexa Fluor 647-conjugated mouse-anti SARS-CoV-2 Spike S1 (R&D Systems, FAB105403R-100UG) at 4°C for 30 min in the dark ...
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bioRxiv - Pathology 2022Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary goat F(ab’)2 anti-mouse IgG-PE antibody used for flow cytometry was purchased from R&D Systems (F0102B) (Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... Quantitation of IL-2 muteins in mouse serum was performed using an electrochemiluminescent immunoassay with a biotinylated rat anti-mouse IL-2 (JES6-1A12, R&D Systems) as a capture reagent and a ruthenylated goat anti-mouse IgG ...
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bioRxiv - Immunology 2021Quote: Plasma cytokine and chemokine profiles from Mif-2−/−Apoe−/− mice and Apoe−/− mice were mapped by capitalizing on a membrane-based mouse cytokine array panel A (R&D Systems) following the standard instructions ...
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bioRxiv - Pathology 2021Quote: Frozen maternal serum aliquots were quick thawed and immediately assayed using either a Human ACE-2 DuoSet ELISA assay (R&D systems) or a ParameterTM Estradiol assay per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-21 and IL-15 were measured in plasma diluted 1:2 from acute COVID-19 patients and acute healthy controls using a custom designed multiplex Magnetic Luminex Screening assay (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Human gastric and colon organoids were generated from deidentified surgical specimens from Stanford Hospital under an approved IRB protocol following established methods and grown in a submerged format in WENR media (Matano et al., 2015; Sato et al., 2011) in BME-2 extracellular matrix (R&D Systems) (Lo et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Neuroscience 2022Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
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bioRxiv - Bioengineering 2024Quote: ... Enzyme-linked immunosorbent assays (ELISA) was then carried out as per manufacturer’s instructions (R&D Systems, BMP-2 DuoSet ELISA kit, DY355). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 2 h at room temperature with secondary antibodies in TBS-T containing 10% milk: anti-Mouse IgG (HAF018, R&D Systems) and anti-Rabbit IgG (A9196 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... TSP2 concentrations in LX-2 cell lysates were determined using enzyme-linked immunosorbent assays following the manufacturer’s instructions (Quantikine® ELISA, #DTSP20, R&D Systems) (16) ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Bioengineering 2024Quote: ... CAR T cells for the initial challenge day 0 imaging experiment were stained with the following antibody mix in FACS buffer (PBS 1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... cells from re-challenge plates were stained with the following antibody mix in FACS buffer (PBS1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Developmental Biology 2021Quote: ... in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin) with added FGF ligands (Fgf7; 4 µM, R&D Systems, Cat. No. 5028-KG-025), the γ-secretase inhibitor DAPT (10 µM ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... caspase-3 (R&D Systems, AF835), Endomucin (Santa Cruz ...
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bioRxiv - Bioengineering 2020Quote: ... TNNI3 (R&D Systems, MAB8594, 1:50), NKX2.5 (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Sox1 (1:50, R&D systems #AF3369); βIII-tubulin/Tuj1 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... SPARCL1 (R&D systems, AF2728, 1:50), SOX10 (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... SPARCL1 (R&D systems, AF2728, 1:50), and DLX5 (Sigma ...