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Citations for R&D Systems :
1251 - 1300 of 1558 citations for Recombinant Caprine IL1A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate were cultured in DMEM base medium supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Immunology 2023Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Immunology 2024Quote: PBMC (2×106 per well) were stimulated in 24-well plates with peptide (individual/pool) plus recombinant IL-2 (R&D Systems Europe Ltd.) at a final concentration of 5µg/mL and 20IU/mL ...
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Systems Biology 2019Quote: ... Sera were subjected to depletion of abundant serum proteins using Proteome Purify 12 Human Serum Protein Immunodepletion Resin (R&D Systems). Denaturing was followed by alkylation with N-ethylmaleimide and acetone precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg total protein was collected from each treated kidney and IL6 protein level was determined by the mouse IL6 duo-set (R&D Systems) ELISA ...
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bioRxiv - Physiology 2021Quote: TNF-α and IL6 protein levels in cell supernatant and plasma IL12/23 and IL17 protein levels were determined using DuoSet Elisa (R&D systems) as per manufacturer’s instruction.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Human 26S Proteasome Protein (E-365) and Human 20S Proteasome Protein (E-360) were purchased from R&D Systems (Minneapolis, MN, USA). Human KRAS (G12D ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Biochemistry 2022Quote: ... mice aged 6.5 weeks were starved for 6 hours and intraperitoneally injected with 220ng/g body weight of recombinant mouse FGF23 (2629-FG-025 R&D Systems, Minneapolis, MN, USA) in PBS-BSA 0.1% or PBS-BSA 0.1% vehicle with injection volume of 4μL/g body weight ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Neuroscience 2019Quote: ... half of the culture media was replaced with media alone or media containing recombinant mouse netrin-1 (R&D systems cat. no.1109-N1/CF), recombinant human ephrin-A5-Fc (R&D systems cat ...
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bioRxiv - Cell Biology 2021Quote: ... For differentiation assays cells were cultured in differentiation media (DMEM + 1% FBS + 1% P/S) with or without recombinant TGFβ (R&D systems, 10 ng/mL) and analyzed 72 hours later by immunofluorescent staining described below ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse tibialis anterior muscles were injected with 50 uL solution of 50,000 human FAPs resuspended in 50% glycerol in PBS with either 29ng/ul concentration of recombinant DLK1 resuspended in PBS (R&D Systems, #1144-PR-025) or with control (identical volume of PBS ...
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bioRxiv - Neuroscience 2024Quote: ... 10-week-old control female and 13-weekold control male mice (Esr1fl/fl) were injected daily with recombinant mouse NOV/CCN3 at 7.48 μg/kg (R&D Systems Catalog # 1976-NV-050) or vehicle control (0.9% NS) ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fibroblasts at 70-80 % confluent were serum starved overnight and treated with 50ng/ml of recombinant human PDGF-DD (R&D system, 1159-SB-025) for 24h.
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bioRxiv - Cancer Biology 2023Quote: ... in combination with different concentrations of inhibitor of IFNα/β (i-IFNα/β) (Recombinant Viral B18R, R&D Systems; 0.9, 1.8 or 3.6 ng/ml) for 40 hours ...
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bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Immunology 2024Quote: ... mice with 20% L929-conditioned medium (LCCM) in DMEM and 20ng/mL recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems, Minneapolis, MN, USA) in RPMI for BMDM and BMDC ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were probed with anti-LXRα (R&D Systems, USA ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 nM Bone Morphogenetic Protein (BMP4, R&D Systems) was added to fresh NIM ...
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bioRxiv - Immunology 2021Quote: ... or rSARS-CoV-2 spike protein (R&D Systems). To detect total IgG or IgA expressing B cells ...
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bioRxiv - Cancer Biology 2021Quote: ... The mPD-L1 protein bought from R&D Systems was used for four rounds of panning ...
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bioRxiv - Cancer Biology 2022Quote: Human Fibronectin Protein (1918-FN-02M, R&D Systems), Fibronectin bovine plasma (F1141 ...
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bioRxiv - Physiology 2019Quote: ... or the protein synthesis inhibitor cycloheximide (R&D Systems) for one hour ...
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bioRxiv - Biochemistry 2020Quote: ... C4BP‐Ang1 or native Angpt1 protein (R&D systems) was mixed with 100 ng Tie2‐Fc (with His tag ...
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bioRxiv - Neuroscience 2022Quote: ... or Fc control protein (R&D Systems, #110-HG) at a final concentration of 2 nM/well for 2 hours before being fixed immediately following.
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bioRxiv - Cancer Biology 2023Quote: ... and carrier-free human CXCL12 protein (R&D systems) at 1:1 molar ratio and the mixture was incubated at room temperature for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... and p24-Gag protein (MAB7360-SP, R&D Systems) (2 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... Plasma C-reactive protein levels were assessed by a Mouse C-Reactive Protein/CRP Quantikine ELISA Kit (R&D Systems, Inc., MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2024Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...