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Citations for R&D Systems :
1251 - 1300 of 2178 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
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bioRxiv - Immunology 2023Quote: ... Just prior to analysis plasma samples were thawed and then assayed for numerous analytes (using dilutions optimized in prior studies) according to the manufacturer’s guidelines: adiponectin using the adiponectin/Acrp30 ELISA (DY1065, R&D System, lower limit of detection [LLoD] 15.6pg/ml) ...
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with anti-hACE2 (R&D Systems) diluted in 1% non-fat milk PBS 0.05% Tween 20 for 2 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Neuroscience 2021Quote: ... The ELISA was performed according to the published protocol [43] using these antibodies: anti-TrkB goat 1:1000 (#AF1494, R&D systems), anti-SorCS2 rabbit 1:2000 (#bs-11963R BIOSS) ...
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bioRxiv - Immunology 2021Quote: ... Then the concentrations of IFNγ in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA) following the manufacturer’s instructions (R&D systems, Inc.).
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bioRxiv - Microbiology 2021Quote: ... IL-1β (cat No. DY401) and IL-12 p70 (cat No. DY419) by using the mouse DuoSet ELISA system (R&D Systems). In addition ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Physiology 2021Quote: TNF-α and IL6 protein levels in cell supernatant and plasma IL12/23 and IL17 protein levels were determined using DuoSet Elisa (R&D systems) as per manufacturer’s instruction.
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bioRxiv - Pharmacology and Toxicology 2021Quote: All samples were assessed by direct-sandwich enzyme-linked immunosorbent assay (ELISA) under the manufacturer’s (R&D System Europe Ltd, Abingdon, UK) according to the National Institute for Biological Standards and Controls (Blanche Lane ...
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bioRxiv - Cell Biology 2021Quote: ... or in the lavage or serum from mice after MSUc injection in the air pouch were measured by ELISA (R&D Systems) following manufacturer’s specifications.
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Cancer Biology 2020Quote: ... conditioned medium was collected and the levels of secreted human CXCL8 and murine CXCL1 were detected with DuoSet ELISA (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: The amount of osteoprotegerin (OPG) released from hMSC-seeded scaffolds was quantified by an OPG ELISA (DY805, R&D Systems, Minnesota, USA). Media was collected every 3 days for 14 days and was pooled and assayed at specific timepoints ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of LCN-2 in the supernatant was measured by using a DuoSet Mouse Lipocalin-2/NGAL ELISA (R&D Systems) kit ...
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bioRxiv - Immunology 2020Quote: TGFβ-1 concentration in supernatant from 4day ILC1 co-cultures and SIO only controls was measured using the Mouse TGFβ-1 DuoSet ELISA (R&D Systems) with modified manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The supernatants from the serovar D infections were subjected to multiplex ELISA analyses in triplicate setting using a custom designed 27-plex human magnetic Luminex assay #LXSAHM (R&D Systems) according to the manufacturer’s instructions ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of TGFβ secreted by mouse pancreatic tumor cells was quantified by using the Mouse TGFβ 1 DuoSet ELISA (#DY1679-05, R&D systems), complemented with Sample Activation Kit 1 (#DY010 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
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bioRxiv - Immunology 2019Quote: ... normal T cell expressed and secreted (RANTES) and tumor necrosis factor alpha (TNF-α) by ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines and chemokines were assayed from organ homogenates using the pro-inflammatory focused 32-plex (Eve Technologies, Calgary, CA) or IFNγ DuoSet ELISA (R&D Systems) according to manufacturer protocol ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Immunology 2021Quote: ... The supernatants were collected 72 hours post-stimulation and the levels of IFNγ and IL-10 determined by and ELISA (R&D Systems). IFNγ production was also assessed by ELISPOT ...
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bioRxiv - Pathology 2021Quote: Frozen maternal serum aliquots were quick thawed and immediately assayed using either a Human ACE-2 DuoSet ELISA assay (R&D systems) or a ParameterTM Estradiol assay per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Immunology 2020Quote: IL-27(p28) in BALF was measured with a mouse IL-27 p28/IL-30 DuoSet ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their coincubation with T2 cells pulsed with MART-126–35 or gp100209-217 [both commercially synthesized and purified (>95%) by reverse-phase HPLC by Biomer Technology] by ELISA (R&D Systems). The MART-126– 35 or gp100209-217–reactive clones were further expanded in a second-round exposure to anti-CD3 (30 ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... IL-8 and TNF from SP140 siRNA or scrambled-treated M1 macrophages (LPS-stimulated or unstimulated) were determined by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA; R&D systems) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... All sera were assessed by direct-sandwich enzyme-linked immunosorbent assay (ELISA) under the manufacturer’s (R&D System Europe Ltd, Abingdon, UK) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
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bioRxiv - Physiology 2023Quote: ... supernatants were collected and the released IL-1β were measured by mouse IL-1β/IL-1F2 DuoSet ELISA (Cat. number: DY401) from R&D Systems.
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bioRxiv - Immunology 2023Quote: Culture media of cells infected with virus were collected at the indicated times and used for ELISA quantification of IFNβ (R&D Systems) in accordance with the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... media was removed and frozen for secretomic screening assays for both VEGF-A (R&D Systems, Human VEGF DuoSet ELISA, Cat# DY293B) and VEGF-C (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Media for the ELISA was immediately stored after collection at -80°C for subsequent testing in the enzyme-linked immunoassay (R&D systems). The Human IL-1β Quantikine ELISA Kit (R&D Systems cat ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 24 and 48 hours intervals from 3 BALB/C mice carrying RCC tumours receiving 1×107 PODS-IL-2 treatment following the final dose and tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Mice were sacrificed 24h post-infection and n=7 were analysed for bacterial load and IL-6 and KC using ELISA (R&D systems). A further n=3 animals were analysed using histology ...
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bioRxiv - Molecular Biology 2023Quote: ... TSP2 concentrations in LX-2 cell lysates were determined using enzyme-linked immunosorbent assays following the manufacturer’s instructions (Quantikine® ELISA, #DTSP20, R&D Systems) (16) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Circulating CCL5 levels were measured in serum from CCR5+/+ mice treated with vehicle or aldosterone via Enzyme-Linked Immunosorbent Assay (ELISA) (R&D System).
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bioRxiv - Immunology 2023Quote: ... Cytokines in the supernatant were determined by fluorescent assays (Ladinig et al., 2014) or a porcine TNF-α ELISA (R&D Systems).
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bioRxiv - Immunology 2024Quote: ... Total THP levels of a subset of urine samples (diluted 1:10) was determined by ELISA (catalog no. DY5144-05, R&D Systems) per manufacturers’ instructions ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... IGF-1 and VEGF-B in tissue homogenates and plasma samples were determined by double-antibody sandwich ELISA according to the manufacturer instructions (IGF-1, MG100 and VEGF-A, MMV00, R&D Systems, USA ...