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Citations for R&D Systems :
1201 - 1250 of 1364 citations for 7 1H Pteridinone 4 amino 2 6 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma concentrations of fatty acid binding protein 4 (FABP4) and leptin were determined using the xMAP technology Luminex assays (R&D Systems, Minneapolis, MN, USA) and the Luminex MAGPIX System (Luminex Corp. ...
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and cardiac fibroblasts were serum starved for 24 h before adding medium containing 5 ng/ml recombinant TGFβ1 for 2 days (R&D Systems, Minneapolis ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... serum was incubated for 2 h with polyclonal sheep IgG anti-LL-37 (2.5 – 20 μg ml−1; R&D Systems) or anti-hNP-1 (2.5 – 20 μg ml−1 ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Fibers were incubated in plating medium consisting of 15% Fetal Bovine Serum (Hyclone) and 2 ng/ml bFGF (R&D Systems) in DMEM.
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bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
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bioRxiv - Neuroscience 2022Quote: ... Group 2 ligands were derived from human transcriptomic data and included: TGFβ2 (R&D Systems, Cat. 302-B2-002, 5ng/mL), NLGN1 (Sino Biological ...
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed three times with PBST and incubated with 15 μl of DAB (3,3’-Diaminobenzidine) chromogen (2 ml of DAB chromogen (860001, R&D Systems) diluted in DAB chromogen buffer (860005 ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... were determined by the CXCL2/MIP-2 DuoSet enzyme linked immunosorbent assay (ELISA) kit to the manufacturer’s instructions (R&D Systems). Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
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bioRxiv - Biochemistry 2022Quote: ... cells were treated for 2 hours prior to infection with the IC50 concentration of anti-ACE2 antibody (R&D Systems: AF933) determined for the cell lines used ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).
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bioRxiv - Developmental Biology 2023Quote: ... media was changed daily with hindgut medium (RPMI 1640, 1X GlutaMAX, 2% defined FBS, 2µM CHIR99021 and 500ng/ml FGF4 (R&D Systems) until day 8 ...
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bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells were pre-treated for 16 h with Type I Interferon (1000 Units/mL; R&D Systems, 11200-2) and treated with HMW Poly(I:C ...
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
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bioRxiv - Cell Biology 2024Quote: ... The organoids were encapsulated in 50% Cultrex basement membrane extract type 2 (BME-II) (R&D Systems; catalog #3533-010-02) and maintained in advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Isolated naive CD4+ T cells were resuspended in isolation media supplemented with recombinant IL-2 (R&D Systems, #202-IL-050) at 10 ng/mL (growth media ...
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bioRxiv - Neuroscience 2024Quote: ... and reseeded on laminin-521 coated plates at a split ratio of 1:2.5 in CPN medium supplemented with 20 ng/mL fibroblast growth factor 2 (FGF2; R&D Systems) and 10 µM Y-27632 ...
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bioRxiv - Cancer Biology 2021Quote: ... culture media were changed to serum free media or serum free media with 2 ug/ml IGF2 antibody (AF-292, R&D Systems) for another 72 hours ...
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bioRxiv - Immunology 2021Quote: Five million of splenocytes per well in 12-well plates were cultured in 2.5 ml of complete DMEM medium (for details refer below) with 1000 U/ml of recombinant human (rh) IL-2 (202-IL-010, R&D Systems) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Bioengineering 2020Quote: Injured animals were randomly selected to receive intralesional injections of eCS matrix functionalized with the neurotrophic factors fibroblast growth factor (FGF-2; 50μg/mL; R&D Systems; 233FB025CF) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... fragments were washed three times in 5 ml cold AdDF+++ before being embedded in 30 μL BME (Type 2; R&D Systems) per well in a 48-well plate ...