Labshake search
Citations for R&D Systems :
1201 - 1250 of 3762 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were cultured using recombinant Human IL-2 (R&D Systems, catalog no. 202-GMP or BT-002-GMP) supplemented at 300 IU/mL and stimulated with 12.5 uL/mL Immunocult Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Each microwell was then overlaid with 2 μL of organoid digestion mix including Organoid Harvesting Solution (R&D Systems) with 10 mg/mL B ...
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bioRxiv - Immunology 2024Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems, 10549-cv) was determined by injecting two to four concentrations of SARS-CoV-2 RBD (50 nM highest concentration) ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-AXL (AF154, IF 1:500, WB 1:1000) from R&D Systems; mouse anti-AKT (2920 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-Sox2 (SC17320, Santa Cruz Biotechnology; 1:300; MAB2018, R&D Systems 1:50), anti-Sox9 (AF3075 ...
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bioRxiv - Biochemistry 2022Quote: ... RhoA (1:500, NBP2-22529 NovusBio) and Klotho (1:1000, AF1819 R&D Systems). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase-conjugated secondary antibodies (Milian Analytica ...
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bioRxiv - Cancer Biology 2021Quote: ... CCL27 (1:200, HIER 1, mouse monoclonal, clone 124308, R&D Systems, USA; MAB376), CCR10 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Mouse sFRP-1 Protein (1 µg/ml, R&D Systems, MN9019-SF-025), Plexin-D1 Fc fragment (30 µg/ml) ...
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bioRxiv - Physiology 2023Quote: ... and Goat anti mouse CD31/PECAM-1 (AF3628-SP, 1:150, R&D Systems) diluted in blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor (Thermo 1:200) and Northern Lights (R&D Systems 1:200) conjugated secondary antibodies were incubated for 1 hour at 37ºC ...
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bioRxiv - Cell Biology 2021Quote: ... SSEA-1 (R&D Systems), TRA-1–60 ...
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bioRxiv - Cell Biology 2023Quote: ... IGF-1 (R&D Systems), and CNTF (R&D Systems).38 Next ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Immunology 2023Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... isolated acinar clusters were resuspended in a stiff matrix composed by 1:1:1 mix of Matrigel-GF reduced : rat collagen I neutralized solution 4.43 ng/mL (R&D Systems – 3440-100-01) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µm sections were deparaffinized and stained with either hematoxylin and eosin or with specific antibodies for PROX1 (R&D Systems, AF2727) and GFP (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... a total of 1 μg native proMMP2 and 0.5 μg proMMP14 were separately incubated in 10 μl with a range of furin (catalog no. 1503-SE-010, R&D systems) concentrations (0 – 250 nM ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Genetics 2023Quote: Reprogramming was done as described previously, combining the IFNγ (10 ng/ml, day 0 to 5) (R&D Systems, 485-MI-100) with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM ...
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bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were incubated with anti-Siglec-9-APC (Clone 191240) and/or anti-Siglec-5/14-AF488 (Clone 194128) (R&D Systems) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Molecular Biology 2022Quote: Human dermal fibroblasts transfected with either 10 nM si-circGLIS3 or scrambled siRNA (Eurofins Genomics, Ebersberg, Germany) for 48 hours and treated with 5 ng/ml TGF-β1 (R&D Systems) were rinsed and pelleted in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: The preosteoblast cells cultured in tissue culture plates were differentiated for 5 days and treated with Myostatin of 100 ng/mL (788-G8-010/CF R&D system), follistatin of 100 ng/mL (769-FS-025/CF ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then washed with phosphate-buffered saline (PBS)/0.05% Tween 20 and incubated with mouse IgG2a-anti-IAV NP (Clone Hb65, EVL laboratories) 5 μg/ml or mouse IgG2a isotype control (MAB003, R&D systems) 2.5 μg/ml in PBS/0.1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated in blocking solution (3% bovine serum albumin + 0.1% Triton X-100 + 5% Horse serum) at room temperature for 1hr and then with anti-OSMR beta (R&D Systems, AF662) antibody (10 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively, R&D Systems). Mouse IL-33 was quantified by ELISA Kit (Cat# 88-7333-22 ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice ...