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Citations for R&D Systems :
1151 - 1200 of 1386 citations for C17orf71 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The slides were fixed in an ice-cold acetone/methanol mixture (50%/50%) and stained with primary anti-mouse CD144 antibody (MAB9381, 1:100, R&D Systems) and secondary donkey-anti-mouse Alexa Fluor® 594 (A21203 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human ACE2 polyclonal antibody (AF933) and mouse anti-human ACE2 mAb MAB9332 were available from R&D Systems (Minneapolis, MA). Rabbit anti-SARS-CoV-2 RBD (40592-T62 ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... The overexpression and loss of ICAM-1 protein were validated by flow cytometry with an anti-ICAM1 antibody (BBA20, R&D Systems). Besides ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µm sections were deparaffinized and stained with either hematoxylin and eosin or with specific antibodies for PROX1 (R&D Systems, AF2727) and GFP (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... cells were wash three times with PBS to remove any residual FBS and followed by 1-hour treatment with goat anti-human ACE2 antibody (R&D Systems) or anti-hDDP4 antibody (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were re-suspended in 100 μl of FACS buffer and incubated with 10 μl of PE-conjugated anti-sheep IgG secondary antibody (F0126, R&D systems) for 30 min on ice ...
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bioRxiv - Systems Biology 2021Quote: ... washed once with FACS buffer (1%BSA in PBS and 5mM EDTA) and incubated for 40 minutes in FACS buffer containing 0.75ug of human ACE2-488 conjugated antibody (R&D Systems FAB9332) and 1:1000 7-AAD (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Developmental Biology 2022Quote: ... microglial cells were preincubated with 20µg/ml of anti-C3aR antibody (Catalog # MAB10417) or control IgG (R&D Systems, Inc. Minneapolis, MN) suspended in DMEM/F12 supplemented with 5% heat-inactivated FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend]; goat anti-myeloperoxidase [goatMPO, AF3667, R&D Systems] ...
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bioRxiv - Neuroscience 2022Quote: Goat polyclonal antibodies against NEGR1 (AF5394) used for immunocytochemistry (IC: 1:100) and immunohistochemistry (IHC: 1:1000) were from R&D systems. Rabbit polyclonal antibodies against NEGR1 (ab101809 ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatant from treated cells were collected and applied to the IP-10 antibody-coated plate for ELISA analysis (R&D Systems) following the manufacture’s instruction.
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bioRxiv - Neuroscience 2021Quote: ... Each anti-VEGF-A injection consisted of 200 μL of 32.5 pM concentration of antibody against mouseVEGF-A164 protein (YU1418071, R&D Systems, Inc.) in saline ...
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bioRxiv - Immunology 2021Quote: Synovial tissue sections were stained using antibodies against CXCL12 (Monoclonal Mouse IgG1, Clone #79018,Catalog Number: MAB350, R&D Systems, Abingdon, UK), CXCR4 (Monoclonal Mouse IgG2B ...
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bioRxiv - Neuroscience 2021Quote: ... The sandwich layout of this TREM2 MSD assay from the bottom to the top is (1) Capture-antibody (1 μg/mL; BAF1729, R&D Systems), (2 ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Developmental Biology 2022Quote: ... Lung lobes were incubated with primary antibody (anti-VWF, rabbit, 1:100, F3520, Sigma and anti-EMCN, goat, 1:200, AF4666, R&D Systems), in 5% donkey serum and 0.5% Triton X-100 in phosphate buffered saline (PBST ...
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary goat F(ab’)2 anti-mouse IgG-PE antibody used for flow cytometry was purchased from R&D Systems (F0102B) (Minneapolis ...
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bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Threshold gates were set with the same samples that were stained with the following isotype control antibodies: Normal chicken IgY Control (R&D Systems), and normal rabbit IgG control (Santa Cruz Biotechnology) ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Synthetic Biology 2020Quote: ... Myc-tagged and V5-tagged CARs were stained using an Alexa Fluor 488-conjugated mouse anti-myc antibody (R&D Systems, IC3696G) and an Alexa Fluor 647-conjugated mouse anti-V5 antibody (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1h at room temperature and probed with primary antibodies against SORCS2 (1:750; AF4238, R&D Systems) and GAPDH (1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed with PBST three times and incubated with 30 μl of biotinylated anti-rabbit secondary antibody for 90 minutes (1:10 dilution, 865002, R&D Systems). After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution ...
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bioRxiv - Immunology 2020Quote: Titration of blocking antibodies was performed by using static adhesion assays in which activated CD4+ T cells were allowed to adhere to either recombinant human ICAM/Fc or VCAM/Fc chimeras in the presence of blocking antibodies specific for integrin α4 (clone 2B4, R&D Systems) or integrin αL (clone TS1/22 ...
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bioRxiv - Developmental Biology 2019Quote: ... Commercially available anti-FLT1 antibodies were obtained from the manufacturer in carrier free and preservative free form (AF471 from R&D Systems, D2 from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175) antibody (catalog no. MAB835; R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2021Quote: ... were coated with anti-IFN-γ antibody (capture mAb; 1 µg/mL) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Plates were blocked using culture medium (DMEM containing 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40; AF3075; R&D Systems) and rabbit polyclonal antibodies against GFAP (1:250 ...
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bioRxiv - Bioengineering 2020Quote: Surface expression of decoy constructs on engineered MSC lines was assessed by using either APC-conjugated rat-anti-mouse gp130 (IL6ST) antibodies (clone FAB4681A, R&D Systems) or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1 ...
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Physiology 2022Quote: ... incubated in primary antibodies (mouse monoclonal anti-HNF4α P1 and P2; catalog no. PP-K9218-00 and PP-H6939-00, respectively, R&D Systems) in 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems, catalog #: PS022), a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were probed by incubation for 1 hour at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592 ...