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Citations for R&D Systems :
1101 - 1150 of 1307 citations for 6 HYDROXY 2 METHYL PYRIMIDINE 4 ACETIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to a membrane using the iBlot2 system and incubated overnight at 4°C with primary antibody (anti-Gli3, 1:500, AF3690, R&D Systems, Minneapolis, MN). Membranes were then incubated in secondary antibody (anti-goat Alexafluor 680 ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Immunology 2022Quote: ... cells were washed at day 7 and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 250 U/ml IFN-α (R&D Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... organoids were grown embedded in a mix of 90% ice-cold RGF BME Type 2 PathClear (Cultrex, R&D Systems) and 10% Dulbecco’s modified Eagle’s Medium/Ham’s F12 (Gibco) ...
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bioRxiv - Immunology 2019Quote: ... Transduced cells were selected for in the presence of 2 µg/ml puromycin (R&D Systems Inc, Minneapolis, MN, USA), live cells harvested by ficoll gradient and flow cytometry used to determine purity based on expression of eBFP indicative of transduction.
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2019Quote: ... cover glasses were coated for 2 h at 37°C with 100 nM recombinant ICAM1-FC (796IC, R&D Systems) and washed 2 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... CA) with 1% human serum and 1,200 IU/ml of recombinant human interleukin-2 (R&D Systems, Inc., Minneapolis, MN). As a positive control ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Bioengineering 2021Quote: ... Virus infection was detected with mouse mAb to SARS-CoV- 2 nucleocapsid (N) (R&D Systems, Minneapolis, MN, Cat#MAB10474) and counterstained with rabbit anti-mouse IgG-HRP (Rockland Immunochemicals ...
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bioRxiv - Developmental Biology 2022Quote: ... the differentiation media consisting of aRB27 with 2% Geltrex or Cultrex (+BME overlay) and 10ng/ml BMP4 (R&D Systems) was added to the cells [or with variations ...
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bioRxiv - Neuroscience 2023Quote: ... Culture media consists of N2B27 base medium supplemented with 20 ng/mL FGF-2 (R&D Systems, 233-FB-010), and 50 units/mL-50ug/mL Penicillin-Streptomycin (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... DIV12 hippocampal neurons were treated with either 2 ug/mL of recombinant Nptx2 (CF 7816-NP-050, R&D Systems) or BSA as control ...
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bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... A feeding layer of 2 ml RPMI growth medium supplemented with 10 ng/ml PDGF-BB ligand (R&D Systems) and/or 0.01 uM siRNA (see below ...
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2023Quote: ... The supernatant was analyzed using the mouse Lipocalin-2 enzyme-linked immunosorbent assay (ELISA) Duoset (DY1857-05, R&D Systems) according to the manufacturer’s instruction in serial dilutions.
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Immunology 2023Quote: ... mouse TNF-alpha DuoSet ELISA (DY410) and DuoSet ELISA Ancillary Reagent Kit 2 (DY008) were purchased from R&D system.
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tissue pellet was resuspended in cold Cultrex Reduced Growth Factor BME type 2 (Cultrex BME) (R&D systems) and 25 mL drops per well were plated on pre-warmed 48-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...