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Citations for R&D Systems :
1051 - 1100 of 4278 citations for 7 7R 7 Amino 5 azaspiro 2.4 hept 5 yl 8 chloro 6 fluoro 1 1S 2R 2 fluorocyclopropyl 1 4 dihydro 4 oxo 3 quinolinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... glass slides or 8 well chambers were coated with either ICAM-1 alone or anti-CD3 (clone Hit3a from Biolegend)/anti-CD28 (clone CD28.2 from Biolegend)/ICAM-1 (R&D systems) in PBS for 2hrs at 37°C to analyze T cell migration or synapse formation ...
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bioRxiv - Cell Biology 2021Quote: ... SSEA-1 (R&D Systems), TRA-1–60 ...
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bioRxiv - Cell Biology 2023Quote: ... IGF-1 (R&D Systems), and CNTF (R&D Systems).38 Next ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Neuroscience 2024Quote: ... day 8 hFpO and day 8 hSpO were transferred to a new well containing neural medium supplemented with EGF (R&D Systems, 236-EG), Retinoic acid (0.1 µM ...
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bioRxiv - Immunology 2021Quote: ... 115-006-068), 250ng/ml mouse recombinant MEGACD40L (Enzo, ALX-522-120) and 20ng/ml Recombinant mouse IL-4 (R&D system, 404-ML). Cells were collected at specific time points for analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Immunology 2023Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... isolated acinar clusters were resuspended in a stiff matrix composed by 1:1:1 mix of Matrigel-GF reduced : rat collagen I neutralized solution 4.43 ng/mL (R&D Systems – 3440-100-01) ...
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bioRxiv - Immunology 2022Quote: ... caspase-8 (50 μM Z-IETD-FMK, R&D Systems) or caspase-9 inhibitors (50 μM Z-LEHD-FMK ...
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bioRxiv - Microbiology 2020Quote: ... IFN-λ1 (100 ng/ml, 8 h, R&D Systems), IL-1a (10 ng/ml ...
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bioRxiv - Molecular Biology 2019Quote: IL-8 was quantified by a commercial (R&D Systems) sandwich ELISA ...
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bioRxiv - Cancer Biology 2020Quote: ... Human IL-8/CXCL8 biotinylated antibody (R&D Systems BAF208) and Human/Primate CXCL1/GROα/KC/CINC-1 biotinylated antibody (R&D Systems BAF275 ...
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bioRxiv - Neuroscience 2022Quote: ... basic fibroblast growth factor (8 ng/mL, R&D Systems), penicillin-streptomycin (1X ...
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bioRxiv - Immunology 2022Quote: ... human IL-8 (#DY208-05) (R&D Systems, Minneapolis, USA) and mouse albumin (#E99-134 ...
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bioRxiv - Microbiology 2023Quote: ... 8 µg of rCD44-Fc (R&D Systems 3660-CD) or rIgG-Fc (R&D systems 110-HG ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µm sections were deparaffinized and stained with either hematoxylin and eosin or with specific antibodies for PROX1 (R&D Systems, AF2727) and GFP (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Biochemistry 2020Quote: ... a total of 1 μg native proMMP2 and 0.5 μg proMMP14 were separately incubated in 10 μl with a range of furin (catalog no. 1503-SE-010, R&D systems) concentrations (0 – 250 nM ...
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bioRxiv - Molecular Biology 2022Quote: Human dermal fibroblasts transfected with either 10 nM si-circGLIS3 or scrambled siRNA (Eurofins Genomics, Ebersberg, Germany) for 48 hours and treated with 5 ng/ml TGF-β1 (R&D Systems) were rinsed and pelleted in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: The preosteoblast cells cultured in tissue culture plates were differentiated for 5 days and treated with Myostatin of 100 ng/mL (788-G8-010/CF R&D system), follistatin of 100 ng/mL (769-FS-025/CF ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then washed with phosphate-buffered saline (PBS)/0.05% Tween 20 and incubated with mouse IgG2a-anti-IAV NP (Clone Hb65, EVL laboratories) 5 μg/ml or mouse IgG2a isotype control (MAB003, R&D systems) 2.5 μg/ml in PBS/0.1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were incubated with anti-Siglec-9-APC (Clone 191240) and/or anti-Siglec-5/14-AF488 (Clone 194128) (R&D Systems) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Genetics 2023Quote: Reprogramming was done as described previously, combining the IFNγ (10 ng/ml, day 0 to 5) (R&D Systems, 485-MI-100) with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated in blocking solution (3% bovine serum albumin + 0.1% Triton X-100 + 5% Horse serum) at room temperature for 1hr and then with anti-OSMR beta (R&D Systems, AF662) antibody (10 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively, R&D Systems). Mouse IL-33 was quantified by ELISA Kit (Cat# 88-7333-22 ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...