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Citations for R&D Systems :
1001 - 1050 of 1543 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Molecular Biology 2022Quote: ... and interleukin-4 (IL-4, 50 ng/mL, R&D Systems, 6507-IL-025/CF) for 5 days ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then cultured with bone morphogenic protein-4 (BMP-4; R&D Systems) and CHIR-99021 in RPMI-B27 medium to specify for cardiac mesoderm lineages ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/mL IL-6 (206-IL-050, R&D Systems), 10 ng/mL VEGF and 30 ng/mL DKK-1(5439-DK-500 ...
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bioRxiv - Microbiology 2021Quote: ... IL-6 (10 ng/mL, all from R&D Systems, MN) and PGE2 (1 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... or CXCL9 (6 μg/ml, #AF-492-NA, R&D Systems) were added to the co-culture system 30 minutes before MPLA with IFNγ.
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bioRxiv - Cancer Biology 2021Quote: ... and 6 µg/mL anti-ULBP3 (clone 166510, R&D Systems) for 1h at 37°C prior to plating with γδ T cells ...
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bioRxiv - Immunology 2021Quote: ... and IL-6 secretion was assessed by ELISA (R&D Systems). Each semi-prep sample (15 µg/ml ...
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bioRxiv - Immunology 2021Quote: ... IL-6 (30 ng/ml; carrier-free; Tocris R&D Systems), IL-1β (10 ng/ml ...
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bioRxiv - Physiology 2022Quote: ... Cells were thereafter lysed with Lysis Buffer #6 (R&D Systems) and assayed for the expression levels of phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Mouse IL-6 Quantikine ELISA Kit (M6000B, R&D Systems), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 at 200 pg/ml (R&D system, 406-ML) was added to the basolateral compartment from day 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/ml rhIL-6 (#206-IL, R&D Systems).30
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bioRxiv - Bioengineering 2024Quote: ... Human IL-6 Quantikine ELISA Kit (R&D systems, MN, USA), Mouse PBMC Isolation Kit (Solarbio ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were blocked for one hour using 10% normal goat serum (R&D systems) with 0.3% Triton-X100 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml; R&D Systems) plus LPS (100 ng/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... every 7 days Matrigel was lysed with Cultrex™ Organoid Harvesting Solution (R&D Systems™, MN), and isolated mGO ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/ml recombinant mouse interleukin-4 (IL-4) (R&D Systems, Cat number: 404-ML) was used (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2021Quote: ... After 48 hours the cells were fed with stage 2 differentiation medium consisting of STEMDiff APEL supplemented with 50 ng/mL of VEGF 165 (R&D Systems, 293-VE-010), 25 ng/mL of BMP4 (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 107 cells per sample were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and incubated with 50 µg/mL SARS-CoV-2 spike S1 subunit-Fc fusion protein (R&D systems, #10623-CV-100) or human IgG1 isotype control (BioXCell ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were infected with HIV-1 (50 ng HIV-p24/106 cells) and then cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA) for up to 9 days ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Immunology 2022Quote: ... Fecal LCN2 levels in the supernatant fraction were estimated using a Duoset murine LCN-2 ELISA kit (R&D Systems DY1857-05, Minneapolis, MN, USA) based on provided directions.
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bioRxiv - Physiology 2022Quote: ... and phospho-JNK (T183/Y185 for JNK1/2 and T221/Y223 for JNK3) with commercial kits (DuoSet IC ELISA, R&D Systems, # DYC1018B and # DYC1387B) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Bioengineering 2023Quote: ... Ang-2 and MCP-1 were measured from individual samples following the manufacturer’s protocols of respective Quantikine ELISA Kits (R&D systems, DVE00, DANG20 and DCP00). Cytokine expression profiles of 2D cultures were assessed using a human angiogenesis antibody array (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were resuspended in 1 part Advanced DMEM/F12 and 2 parts Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems; 3533-010-02) for a final solution of 66% Cultrex ...
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bioRxiv - Immunology 2020Quote: ... recombinant IL-4 (R&D systems), and anti-CD40 (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-4-HNE (R&D Systems) was used with an AF647 secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... t-PA + plasminogen (R&D Systems, UK; 6, 12 or 120 nM) or without proteases for 15 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... IL-6 (25ng/mL) and IL-23 (20ng/mL, R&D Systems). For Th1 differentiation cultures ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in ice-cold Lysis Buffer 6 (R&D systems) supplemented with 10 μL/mL of protease inhibitor cocktail ...
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bioRxiv - Immunology 2021Quote: ... IL-6 (1000 U/mL; R&D Systems Cat# 206–1 L), and PGE2 (2 μM ...
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bioRxiv - Genomics 2022Quote: ... and IL-6 (10ng/mL; R&D Systems Cat. 406-ML-005) at 37°C for 2 hours.
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bioRxiv - Molecular Biology 2023Quote: ... An ELISA kit was used to measure IL-6 (R&D Systems) and we performed experiments following the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... and IL-6 release using Mouse DuoSet ELISA kits (R&D Systems, Minneapolis ...