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Citations for R&D Systems :
1001 - 1050 of 1459 citations for 6 methoxy 2 phenyl 3 2H tetrazol 5 ylmethylsulfanyl 1H indole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... following 5 min of stimulation with 10 ng/mL PDGF-AA or PDGF-BB (R&D Systems) for the PDGFRα homodimer and PDGFRβ homodimer cell lines ...
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bioRxiv - Genetics 2020Quote: ... sections were incubated with 5 μg/ml goat anti-mouse CD31 (R&D Systems, Inc, Minneapolis, MN) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stimulated overnight with 5 pg/ml of BMP9 in serum-free medium (R&D Systems) and luciferase activity was measured with the twinlite Firefly and Renilla Luciferase Reporter Gene Assay System (PerkinElmer) ...
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bioRxiv - Developmental Biology 2023Quote: ... transfected cells were provided with 5 ng recombinant mouse TGFβ1 protein (R&D Systems, 7666-MB-005). mCherry fluorescence was determined first by exciting at 570 nm and measuring emission at 610 nm with a 100 msec time delay using a Perkin-Elmer EnVision Xcite Multilabel Plate Reader ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were treated with 100 ng/mL mouse IFNγ or 5 U/mL IFNα (R&D Systems) for 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... heating at 95°C for 5 min in antigen retrieval solution (CTS045, R&D Systems, Minneapolis, MN). After blocking non-specific binding in 2% non-fat milk in 0.1 M PBS containing 0.1% Triton X-100 (PBS/MT ...
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bioRxiv - Cell Biology 2023Quote: ... the pro- and undifferentiated spermatogonia marker PLZF (day 5-35; 0.6 µg/ml, R&D Systems AF2944), and dual stained with the transcription factor marker FOXO1 (day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with either DMSO or 5 µM Idasanutlin (R&D Systems, #12-35-22-07) for an additional 24 h before cells were harvested for protein and RNA extraction.
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bioRxiv - Immunology 2024Quote: ... dFBs were treated with either recombinant mouse IFNγ (5 ng/ml, R&D Systems, Minneapolis, MN, USA) for 8 or 24 hours before being subjected to qRT-PCR or ELISA analysis.
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bioRxiv - Cell Biology 2023Quote: ... following 5 min of stimulation with 10 ng/ml PDGF-BB (R&D Systems, Minneapolis, MN, USA) in the case of the PDGFRαV1/βV2 heterodimer cell line ...
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bioRxiv - Genetics 2021Quote: ... and 2 μg/ml of mouse anti-ACE2 (R&D Systems;; Catalogue: MAB933;; RRID: AB_2223153). Secondary antibodies used were ...
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bioRxiv - Immunology 2021Quote: ... plates were blocked in 300 ml of 1x reagent diluent concentrate 2 (R&D Systems) for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... Replacement of CHIR99021 by 20 ng/mL FGF-2 (223-FB-500, R&D Systems), followed by maintenance in 15 ng/mL VEGF and 5 ng/mL FGF-2 induced differentiation into the hematopoietic lineage according to a modified protocol from Grigoriadis et al ...
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bioRxiv - Immunology 2021Quote: ... cells have been expanded using 20ng/ml mouse IL-2 (R&D System 402-ML). Cells were considered into exhaustion-like state after 4 stimulations (Tex ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with growth factors at the following concentrations: LM-22A4 (2 μM, R&D Systems), GDNF (2 ng/mL ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with growth factors at the following concentrations: LM-22A4 (2 µM, R&D Systems), GDNF (2 ng/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were diluted at 2 μg/ml using Calibrator Diluent RD5-18 (R&D Systems). Hyaluronan quantification was then performed using the Hyaluronan Quantikine™ ELISA kit from R&D Systems (DHYAL0).
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bioRxiv - Neuroscience 2022Quote: ... and 2 ng/ml recombinant human NRG1-β 1 (#396-HB, R&D Systems, USA) as described in Poitelon et al ...
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bioRxiv - Molecular Biology 2022Quote: Human recombinant BMP-2 and BMP-4 were purchase from R&D Systems (Minneapolis, MN). Anti-Myc and anti-HA antibodies were purchased from Cell Signaling Technology (Danvers ...
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bioRxiv - Cancer Biology 2022Quote: ... using additional reagents from the DuoSet ELISA ancillary reagent kit 2 (R&D Systems DY008). Briefly ...
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bioRxiv - Immunology 2020Quote: ... isolated NK cells were cultured in 1000 IU/mL human IL-2 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000, R&D Systems, Cat#MAB10474) was used for the detection of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... the secretion levels of Matrix metallopeptidase (MMP)-2 and -9 (R&D system, MN, USA) and transforming growth factor (TGF)-β1 (R&D system ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 uM SB431542 (SA) and 10 ng/mL recombinant human FGF-2 (R&D Systems) were added on D2 for 2 days ...
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bioRxiv - Immunology 2022Quote: IL-4 complexes were generated by incubating 2 μg mouse rIL-4 (R&D Systems) with 10 μg LEAF purified anti-mouse IL4 antibody (clone 11B11 ...
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bioRxiv - Systems Biology 2023Quote: ... Tube 2: rat monoclonal anti-human GSK-3B(Clone 272536, catalog # MAB2506, R&D Systems) and rabbit polyclonal anti-human Phospho-GSK-3BCatalog # AF1590 ...
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bioRxiv - Immunology 2023Quote: ... were coated with human ACE-2 Fc chimera protein (R&D Systems cat# 10544-ZN) in cold PBS (100 μl per well ...
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bioRxiv - Genetics 2023Quote: ... as per the manufacturer’s instructions (Mouse TGF-beta 2 Quantikine ELISA Kit, R&D Systems). The samples were diluted (1:80) ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Immunology 2020Quote: ... supernatants were collected and secretion of TGF β and MMP2/3/9 was assessed in the supernatant by TGFβ1 ELISA kit (R&D systems) or MMP2/3/9 luminex kit (eBiosciences ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Microbiology 2020Quote: ... hESCs were cultured to achieve 80-90% confluency, and treated with 3 μM CHIR99021 (CHIR, Stem-RD) and 100 ng/ml Activin A (R&D systems) in basal medium RPMI1640 (Cellgro ...
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bioRxiv - Systems Biology 2021Quote: ... HGF 50 ng/ml; NT-3 10 ng/ml; PDGF-BB 10 ng/ml; GDNF 10 ng/ml; All from R&D systems). Ligand treatment was started at the time of tetracycline induction ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative cytokine content of the decellularized mouse heart tissues (n=3) were obtained using the Mouse XL Cytokine Array Kit (ARY028, R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Microbiology 2019Quote: ... Cell supernatants were collected and levels of IFNλ were determined with the IFN-lambda 1/3 DuoSet ELISA kit (R&D Systems). Plates were read on a Synergy HT plate reader (Biotek ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...