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Citations for R&D Systems :
1001 - 1050 of 1191 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 ng/ml Activin [cat. num. 338-AC; R&D Systems, Minneapolis, MN], 20 ng/ml FGF2 [cat. num. 233-FB-025/CF; R&D Systems, Minneapolis ...
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bioRxiv - Physiology 2021Quote: ... HOMA-IR was calculated as (fasting glucose × fasting insulin)/22.5 and HOMA-B as (20 × fasting insulin)/(fasting glucose – 3.5).(31) GDF15 concentration was measured with Human GDF-15 Quantikine Elisa Kit (R&D Systems, Minneapolis, MN, USA). Serum GDF15 samples during and after pregnancy were diluted 1:64 and 1:4 ...
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bioRxiv - Immunology 2021Quote: ... magnetically isolated T cells were resuspended in the complete medium and were seeded in a 10-cm petri dish or T-75 flask at 1–2×106 cells/cm2 and cultured in the presence of 20 ng/mL recombinant mouse interleukin-7 (rmIL-7; 402-ML-020/CF, R&D Systems, Minneapolis, MN) for 24 hr at 37°C in a humidified 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2024Quote: ... The supernatants were kept at −20°C and assessed for IFN-γ secretion using the Human IFN-γ DuoSet ELISA kit (DY285B, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... the cells undergoing differentiation were cultured in basal medium supplemented with 5% v/v Osteogenic Supplement (R&D Systems) with the control cells cultured in basal medium ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (R&D Systems, Minneapolis, USA), and separately in combination with IL-4 and IL-21 (100 IU each) ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM) from R&D Systems (R&D252-PL-050). The final volume within each well was 100 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Immunology 2024Quote: ... and incubated with 5 ng/mL IL-4 (cat. no. 404-ML-010, R&D Systems, Minneapolis, MN, USA) (or vehicle control ...
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bioRxiv - Cell Biology 2024Quote: ... were coated overnight at 4°C with 5 ug/ml recombinant Invasin proteins or 2% BME (R&D systems) in cold PBS milliQ ...
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Cancer Biology 2020Quote: ... CHLA9 and CHLA10 cells were cultured in IMDM +L-glutamine media supplemented with 20% FBS and 1% insulin-selenium-transferrin (ITS, R&D Systems, cat no: AR013). Cell lines were maintained at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Organoids were subsequently differentiated towards cartilage by culturing them in the presence of 20 ng/ml BMP4 (R&D Systems, cat. no. 314-BP-010) for 6 days.
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bioRxiv - Biochemistry 2023Quote: FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/ml rhIL-17A (R&D Systems, #7955-IL-025/CF), rhIL-17B (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... Transiently transfected Jurkat cells in a volume of 500 μL were incubated in the absence or presence of the activators: TNF-α (20 ng/mL; R&D Systems, Minneapolis, MN), Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Bioengineering 2023Quote: ... They were then perfused through the renal vein at 100 mL/min with a pressure less than 20 mmHg in custom antibiotic-free endothelial media containing endothelial basemedia (R&D Systems, Minneapolis, MN, #CUST01707), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... mice with 20% L929-conditioned medium (LCCM) in DMEM and 20ng/mL recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems, Minneapolis, MN, USA) in RPMI for BMDM and BMDC ...
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bioRxiv - Developmental Biology 2024Quote: ... The hPSCs were plated at ∼4x104 cells/cm2 and allowed to grow for 2 days before changing with “Day 0 medium” consisting of the “base medium” plus 20 ng/mL BMP4 (R&D Systems 314-BP-050), 30 ng/mL Activin A (R&D Systems 338-AC-050) ...
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bioRxiv - Cell Biology 2020Quote: ... The chamber was prepared by adding 26μl pre-warmed (37°C) CXCL-5 (0, 0.5, 50, 500ng/ml) or CCL2 (0, 0.5, 50, 500ng/ml) (R&D systems) in DMEM to the lower chamber ...
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bioRxiv - Microbiology 2021Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minnesota, MN). For total and differential cell counts ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Immunology 2020Quote: ... two group of 5 mice each were treated with monoclonal anti-mouse IL-25 (mα-IL-25) (R&D Systems) or monoclonal anti-mouse IL-4Rα (mα-IL-4Rα ...
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bioRxiv - Microbiology 2023Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minneapolis, MN). For total and differential cell counts ...
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bioRxiv - Physiology 2023Quote: ... intra-assay % coefficient of variation [%CV] 5%-2.5%), alanine transaminase (ALT) (Siemens Healthcare, intra-assay %CV 8.1%-3.6%) and growth differentiation factor 15 (GDF15) (R&D Systems, BioTechne ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Bioengineering 2024Quote: ... the mTeSR Plus medium was replaced with a mesoderm layer induction medium containing 5 µM CHIR99021 (R&D Systems; 4423), 25 ng/mL bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody for targeting vasculature (Mouse CD31/PECAM-1 Polyclonal Goat IgG, Cat: AF3628; R&D Systems, 1:5 dilution) and antibody against pimonidazole adducts (conjugated IgG1 rat monoclonal antibody clone 11.23.22.r ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981; IFNλ2/3: R&D systems, MAB1587), or mock ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Neuroscience 2024Quote: ... HEK-293 cells 72h posttransfection or adult DRG neurons at DIV 3 were incubated with commercial anti-CASPR2 antibodies (AF5145, 1:250, R&D Systems, Minneapolis, MN, US) and/or human patient serum (1:50-1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...