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Citations for R&D Systems :
1001 - 1050 of 3699 citations for 3 Methyl 1 6 naphthyridine 2 carboximidamide hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC at 60-70% confluence were treated for 3 days with 100 ng/mL Activin A and 100 ng/mL bFGF (R&D Systems) in the presence of increasing levels of FBS (0% on day 1 ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Developmental Biology 2023Quote: ... rmSlit-1 was used at 200 ng·mL-1 and rmEphrinA5 at 500 ng·mL-1 (R&D Systems). Spermine-NONOate was used at 50mM ...
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bioRxiv - Developmental Biology 2023Quote: ... rmSlit-1 was used at 200 ng·mL-1 and rmEphrinA5 at 500 ng·mL-1 (R&D Systems). Images were acquired in DIC every 15 sec by using a Prime95B camera (Photometrics) ...
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bioRxiv - Cell Biology 2024Quote: ... and rat anti-laminin alpha 1 / beta 1 mAb (1:100) (R&D Systems, Minneapolis, MN, USA) was used for the primary antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Nkx6-1 (Goat 1:200; R&D Systems AF5857). Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Nkx6-1 (Goat 1:300; R&D Systems AF5857), anti-Flag (Mouse HRP ...
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bioRxiv - Microbiology 2021Quote: ... FIZZ-1/RELM (1:1000, Ref. AF1523, R&D Systems), pCREB (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... LYVE-1 (1:100, AF2089, R&D Systems, Minnesota, USA) CD32B (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... TuJ-1 monoclonal (R&D systems, Cat# MAB1195, 1:500); Alexa Fluor 568 conjugated phalloidin (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2023Quote: ... 1% normal rat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a humid chamber with the appropriate antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-Netrin-1 antibody 1:500 (R&D Systems AF1109) in 1% BSA + 0.1% PBST was incubated at 4C overnight ...
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bioRxiv - Pathology 2024Quote: ... anti-mouse Mac-1 (R&D Systems, dilution 1:100) or anti-mouse CD3 (Abcam ...
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bioRxiv - Immunology 2023Quote: ... LOX-1 (clone 214012, 1:50) from R&D Systems; Siglec-E (clone M1304A01 ...
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bioRxiv - Neuroscience 2023Quote: ... intercellular adhesion molecule 1 (ICAM-1) (R&D systems, DY796), and E-selectin (R&D systems ...
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bioRxiv - Genomics 2023Quote: ... 1% normal goat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a wet chamber with the appropriate antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-Neuropilin-1 (R&D Systems AF566 1:800), rabbit anti-p73 (Cell signaling 14620 1:250) ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-neuropilin-1 (1:200, AF566; R&D Systems), goat anti-podocalyxin (1:200 ...
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Molecular Biology 2020Quote: ... and TNF-α-treated CM with FGF-2 neutralizing antibody (NAb; AF-233, R&D Systems). On day 7 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were immuno-precipitated with 2-5 µg of either anti-GFI1 (AF3540, R&D systems), anti-H3K4me1 (ab8895 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were exposed for 2 days to human Activin A (8 ng/ml, R&D Systems) and human bone morphogenetic protein 4 (hBMP4 ...
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bioRxiv - Immunology 2020Quote: ... then washed and stimulated in the same media with 5ng/mL hIL-2 (R&D Systems), 12ng/mL hIL-10 (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... in defined media supplemented with 2% B27 and 20ng/ml EGF and bFGF (R&D System). Neurospheres were passaged once and conditioned medium was recovered before the second passage ...
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bioRxiv - Microbiology 2021Quote: ... The cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... and soluble receptor interleukin 2 (sCD25) were analysed using the Human Quantikine Immunoassay (R&D Systems). All following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... we then stained with 2 μg of anti-ACE2 goat IgG antibody (R&D Systems, AF933) per million cells followed by a FITC-conjugated secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human TGF-β1 and mouse or human IL-2 were purchased from R&D system or Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Th0 cell cultures were provided IL-2 (15 U/mL; R&D Systems 202-IL-500), while Th17 cell cultures were supplemented with IL-6 (20 ng/mL ...
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bioRxiv - Neuroscience 2023Quote: ... and human fibroblast growth factor 2 (hFGF2) (20 ng/mL, R&D systems 233-FB-025) freshly added into the media ...
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bioRxiv - Immunology 2023Quote: ... Lipocalin-2 (NGAL) was assessed with a Quantikine ELISA Kit (Biotechne R&D Systems, Minneapolis MN). Additionally ...
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bioRxiv - Immunology 2023Quote: ... CTL medium containing 100 IU/ml human IL-2 (R&D Systems, 202-IL-010/CF) was added to the plates and the expansion of T cells continued ...
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bioRxiv - Cell Biology 2024Quote: ... Then plated into Matrigel domes (Cultrex PathClear reduced growth factor BME, type 2; R&D Systems) in a 24 well plate ...
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bioRxiv - Neuroscience 2024Quote: ... or 2 μg of mouse anti-ERRγ antibody (R&D Systems #PP-H6812-00, one replicate) in CUT&RUN Wash buffer for 10min with rotation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 ng/ml glial cell-derived neurotrophic factor (GDNF, R&D Systems, Minneapolis, MN, USA). The experiments were conducted on flasks or multi-well plates (Nunc ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then incubated with 2 μg of anti-galectin-9 (AF2545, R&D systems) or isotype control under rotation ...
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bioRxiv - Cell Biology 2024Quote: ... AOs were dissolved away from Cultrex® RGM Type 2 (R&D systems, #3533-010-02) and fixed in 4 % paraformaldehyde (PFA ...