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Citations for R&D Systems :
51 - 100 of 1238 citations for ssc mir 17 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in a humidified incubator at 37∘C and 5% CO2 and tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems). HEK293T cells ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatant was collected and centrifuged (3000 rpm, 5 min, RT) and Secreted IL6 was measured using the Quantikine® ELISA kit (R&D Systems), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were genetically authenticated by the Australian Cancer Research Foundation (ACRF) Cancer Genomics Facility (Adelaide, Australia) and determined mycoplasma-free using the MycoProbe mycoplasma detection kit (CUL001B, R&D Systems, Minneapolis, MN).
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... detection was performed with the addition of tetramethylbenzidine (R&D Systems) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... anti-VEGF and anti-TGF-β detection antibodies (R&D Systems) diluted in BSA 1% (1:1000) ...
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bioRxiv - Microbiology 2024Quote: ... detection was performed with the addition of tetramethylbenzidine (R&D Systems) for 30 min at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... Levels of lymphocyte-derived cytokines (IFNγ, IL-17) were determined after 7 days (PeliKine Compact or Duoset ELISA, R&D Systems). Baseline inflammatory plasma markers from both cohorts ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... Fixed cells were stained with 1:100 dilution of APC-conjugated anti-SOX-17 (R&D Systems, Minneapolis, MN, USA; IC1924A) for 30 mins at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Media was harvested at indicated times post infection and IFNβ levels were quantified using a standard curve via Mouse IFN-beta DuoSet ELISA kit (R&D systems) using manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... to capture and biotinylated anti‐ANG1 antibody for detection (R&D System). The clones showing the highest titers of the recombinant protein were selected ...
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bioRxiv - Neuroscience 2021Quote: Protein was harvested from WT and p.Glu355Lys iPSC and NCC lysates (d19 of differentiation) using the manufacturer’s Receptor Tyrosine Kinase (RTK) array protocol in Lysis buffer 17 (R&D Systems, # ARY001B). RTK arrays were blocked for 1 hour at room temperature in Array Buffer 1 (manufacturer’s protocol) ...
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bioRxiv - Genomics 2023Quote: ... were assessed from the culture supernatant of the activated CD4+ T cells by Human Th9/Th17/Th22 Luminex Performance Assay 17-plex Fixed Panel (R&D systems, USA). The assay was performed according to manufacturers instructions and samples diluted 1:2 with calibrator diluent ...
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bioRxiv - Neuroscience 2021Quote: ... Following incubation with the detection antibody (biotinylated anti-TREM2; R&D systems, BAF1729) for 1hour at RT and Streptavidin SULFO TAG™ (MSD ...
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bioRxiv - Cell Biology 2022Quote: ... and a human detection OPG antibody (part of cat#DY805, R&D Systems) (29) ...
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bioRxiv - Cell Biology 2020Quote: ... incubated 1 h in RT with HRP-conjugated secondary antibodies (R&D Systems), and followed by rinsing 3x 5 minutes with TBS-T ...
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bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
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bioRxiv - Bioengineering 2022Quote: ... plates were incubated with respective detection antibodies diluted in reagent diluent (R&D systems) (anti-Apis mellifera defensin-1 ...
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bioRxiv - Immunology 2022Quote: ... a culture supernatant was harvested for detection of the cytokines using ELISA (R&D Systems).
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bioRxiv - Cancer Biology 2021Quote: ... Human Cyto C Immunoassay 96-well plates followed by ELISA detection (R&D Systems, Minneapolis, MN). The fractional release of mitochondrial cytochrome c was calculated for each condition as [mean intensitysupernatant/(mean intensitymitochondria + mean intensitysupernatant)] ...
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bioRxiv - Microbiology 2020Quote: ... Positive control for mature IGF2 detection was 500 ng of recombinant mouse IGF2 (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... plates were incubated with biotin-linked anti-IL-1β detection antibody (R&D Systems, 1:1000), followed by horseradish peroxidase-conjugated streptavidin ...
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bioRxiv - Neuroscience 2023Quote: ... as capture antibody and a sulfo-tagged detection antibody (sheep anti-mouse Trem2, AF1729 R&D Systems). MSD plates were developed using 2x MSD read buffer T ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Developmental Biology 2021Quote: ... the wells were plated with 25µl detection antibody (Biotinylated Goat Anti-Mouse IGF-II, R&D Systems – 840963), diluted to 0.72 µg/ml in PBS ...
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bioRxiv - Immunology 2022Quote: ... Detection of IL-13 and IL-4 in lung homogenates was determined using Duoset ELISA (R&D system) as per manufacturers instructions.
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times with 1× Wash Buffer (R&D Systems) to remove unbound proteins ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of a solution containing 0.001 mg/mL of the detection biotinylated polyclonal antibody (BAF1559; R&D Systems) raised against NOTCH3 ECD and 2.5% BSA was added and was used for detection followed by agitation for 2h at RT ...
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bioRxiv - Systems Biology 2020Quote: ... Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Cancer Biology 2020Quote: ... Death-related proteins were measured in cell lysates by a solid-phase antibody array with chemiluminescent detection (R&D Systems, 893900).
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bioRxiv - Cell Biology 2019Quote: ... Luminex® Multiplex and ELISA assays for the detection of chemokines and cytokines were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at RT with anti-PAR/pADPr monoclonal antibody (R&D Systems cat# 4335-MC-100) diluted 1:250 in 3% BSA-PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated for 2 h at room temperature (RT) with biotinylated polyclonal goat anti-human TREM2 capture antibody (0.1 μg/ml; AF1828, R&D Systems). Following incubation with streptavidin-HRP (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: The production of NO was measured using a colorimetric indirect method based on the detection of nitrite (nitrate was initially converted to nitrite by nitrate reductase) as a product of the Griess reaction (R&D Systems) in the supernatants of cell cultures ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Molecular Biology 2021Quote: Retinal organoids on day 90 of differentiation were incubated at RT for 1 hour in PBS with either a goat anti-ACE2 antibody (AF933, R&D Systems) or a normal goat IgG control (AB-108-C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked in PBS and Tween-20 with 1 % (w/v) nonfat dry milk for 1 h at RT followed by incubation with 1:50 PAX6 (AF8150; R&D Systems) or 1:500 FOXC1/2 (CPA3482 ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2021Quote: Secreted IL-4 was measured using a matching antibody pair (capture, Tonbo 70-7041-U500; detection, eBio # 13-7042-85) with color generated using streptavidin-HRP (R&D Systems, Dx998) followed by Ultra-TMB reagent (Pierce ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 1μg of mouse serum IgG for 10 min at RT then incubated with: AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), APC mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...