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Citations for R&D Systems :
51 - 100 of 2461 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 10 U/mL human rIL-2 (202-IL, R&D Systems) was added to expand the T cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 30 IU/mL recombinant human IL-2 (R&D Systems). Following isolation ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Cell Biology 2020Quote: ... IL-2 concentration was measured using a human IL-2 ELISA kit (R&D Systems, DY202-05) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... His (R&D Systems MAB050-100), COX2 (Abcam ab15191) ...
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bioRxiv - Cancer Biology 2020Quote: ... His (R&D Systems MAB050-100). Coverslips were incubated with the primary antibody at 4°C overnight and then washed five times for 5 minutes each in PBS following which ...
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bioRxiv - Cell Biology 2020Quote: ... PE anti-human ULBP-2/5/6 (Clone 165903, R&D Systems), FITC Mouse IgG1 κ Isotype Control (Clone MOPC-21 ...
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bioRxiv - Neuroscience 2020Quote: ... 2 ng/ml Nrg1 (human NRG1-β1 extracellular domain, R&D Systems), and 2 μM forskolin ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibroblast growth factor 2 (20 ng/mL, R&D Systems). hECSR was added to the bottom channel prior to addition of cells into the well ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant human FGF-2 was purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Immunology 2020Quote: ... affinity-purified polyclonal α-human-Ace-2 antibody (R&D Systems #AF933), and recombinant human Ace-2 (R&D Systems #933-ZN-010 ...
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bioRxiv - Immunology 2020Quote: ... Pen-Strep and human recombinant interleukin-2 (210U/mL, R&D systems). For activation ...
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bioRxiv - Immunology 2024Quote: ... recombinant human IL-2 (R&D systems, cat # 202-IL-010/CF), and SIINFEKL peptide (0.5ug ...
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bioRxiv - Immunology 2023Quote: ... and recombinant human IL-2 (rhIL2,50 U/mL; R&D Systems/CellGenix)) with or without 1% penicillin/streptomycin/l-glutamine (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... CA) with 1% human serum and 1,200 IU/ml of recombinant human interleukin-2 (R&D Systems, Inc., Minneapolis, MN). As a positive control ...
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bioRxiv - Cell Biology 2023Quote: ... EBs were formed in an ESC medium depleted of recombinant human FGF-2 (rhFGF-2) (R&D Systems) on day 0 ...
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bioRxiv - Immunology 2024Quote: ... or CEFTA were supplemented with 10IU/mL of recombinant human IL-2 (rh IL-2; R&D Systems) and 10ng/mL of recombinant human IL-7 (rh IL-7 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IL-2 protein was purchased from R&D Systems (Minneapolis, MN). Recombinant mouse Flt3 ligand (Flt3L ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IL-2 protein was purchased from R&D Systems (Minneapolis, MN). Recombinant mouse Flt3 ligand (Flt3L ...
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bioRxiv - Immunology 2020Quote: ... anti-human IL-37 (2 μg/ml, goat polyclonal, R&D Systems, AF1975), anti-human caspase-1 p20 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... 50 ng/ml recombinant human Wnt3a (R&D Systems; day 1 and 2), 1 mM L-Glutamine (L-Glu ...
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bioRxiv - Cancer Biology 2022Quote: ... and recombinant human IL-2 20 ng/ml (202-IL, R&D Systems). The QCM Leukocyte Migration Assay (MilliporeSigma ...
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bioRxiv - Immunology 2024Quote: ... recombinant human IFN-γ (2 ng/ml) (285-IF-100, R&D Systems), recombinant human TNFα (10 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... hBTLA-6×His tag (R&D systems) and hLIGHT-6×His tag proteins (made by the methods described above ...
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bioRxiv - Biophysics 2024Quote: ... Anti-HIS antibody (R&D systems, BAM050) coated polystyrene beads were prepared as previously described.39 The beads were decorated with native purified Dsn1-6HIS-3Flag kinetochores ...
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bioRxiv - Cell Biology 2020Quote: ... during 2 hours and stimulated with human recombinant VEGF165 (25ng/ml, R&D System) during 30 minutes.
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Bioengineering 2020Quote: ... and recombinant human IL-2 (50 U/mL, R&D Systems, Minneapolis, MN, USA). Single cell suspensions were activated with 1 μg/ml Concavalin A (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q) with recombinant human IL-2 protein from R&D Systems (#202-IL). After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971 ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 30 U/mL recombinant human IL-2 (R&D Systems #202-IL), and 55 μM beta- mercaptoethanol (Thermo # 21985023) ...
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bioRxiv - Immunology 2019Quote: ... overnight before addition of 500 IU/ml recombinant human IL-2 (R&D Systems).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-2 (902-MP-010) was purchased from R&D systems (USA). Recombinant human MMP-3 (SRP7783 ...
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bioRxiv - Pathology 2021Quote: ... Samples were assayed into a Human ACE-2 DuoSet ELISA assay (R&D systems), loading equal amounts of protein in duplicate for all samples.
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bioRxiv - Bioengineering 2022Quote: ... or 150 ng/ml recombinant human LOX homolog like 2 (LOXL2, R&D Systems), with media changed every 2-3 days ...
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bioRxiv - Immunology 2022Quote: ... 200 IU/ml of recombinant human IL-2 (R&D Systems, 202-IL-050) and expanded for 10 – 14 days ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems) and then cultured in ultra-low attachment 6 well plate (Corning ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-human angiotensin converting enzyme 2 (ACE2; R&D Systems; 1:500). Appropriate species-specific secondary antibodies conjugated to AlexaFluor555 and/or AlexaFluor647 were applied for fluorescent detection ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant human transforming growth factor-β (TGF-β) and fibroblast growth factor-2 FGF-2 were purchased from R&D Systems. BTZ ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-His primary antibody (R&D systems) was used at 1:1000 dilution in TBS ...
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bioRxiv - Biochemistry 2020Quote: ... His-ubiquitin (R&D Systems; Cat. # U-530), HA-ubiquitin (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... His-ubiquitin (R&D Systems; Cat. # U-530), HA-ubiquitin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... His-HBP was purchased from R&D Systems. Purified His-HBP was incubated with beads bound with GST (control) ...
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bioRxiv - Cell Biology 2023Quote: ... with 10% HI-FBS (R&D Systems S11150), 100x Pen/Strep (Gibco 15140-122) ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...